Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,375,973 |
G→A |
G369S (GGC→AGC) |
dcuD → |
putative transporter DcuD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,375,973 | 0 | G | A | 100.0%
| 117.1
/ NA
| 33 | G369S (GGC→AGC) | dcuD | putative transporter DcuD |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/13); total (20/13) |
GGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATG > NC_000913/3375897‑3376039
|
ggAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGctc > 1:638380/1‑81 (MQ=255)
aGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTg < 2:638305/81‑1 (MQ=255)
aTTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGc > 1:638378/1‑80 (MQ=255)
ggCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGttt < 2:638307/81‑1 (MQ=255)
ggCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGtt < 1:638306/80‑1 (MQ=255)
ggTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATg > 1:638353/1‑81 (MQ=255)
tATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGt > 2:638389/1‑80 (MQ=255)
aTTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCa > 2:638367/1‑81 (MQ=255)
ttGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCAt < 1:638308/81‑1 (MQ=255)
gTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGTCGTTTATGTCATTTGc > 1:638362/1‑80 (MQ=255)
gTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGc > 1:638373/1‑80 (MQ=255)
gTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGc > 1:638360/1‑80 (MQ=255)
gatgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAg > 2:638387/1‑81 (MQ=255)
tgatTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTc < 1:638309/81‑1 (MQ=255)
attattATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCtt < 2:638310/81‑1 (MQ=255)
ttattaTGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTa < 1:638311/81‑1 (MQ=255)
attatGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTAt < 1:638312/80‑1 (MQ=255)
atGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTcc < 2:638313/80‑1 (MQ=255)
ggCGCTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGaa > 2:638359/1‑81 (MQ=255)
gcgcTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGaa > 1:638375/1‑80 (MQ=255)
gcgcTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGaa < 2:638314/80‑1 (MQ=255)
gcTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAatat > 2:638382/1‑81 (MQ=255)
gcTGGTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAatat > 1:638368/1‑81 (MQ=255)
ggTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCg > 1:638371/1‑80 (MQ=255)
gTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCg > 1:638365/1‑79 (MQ=255)
gTCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGc < 1:638315/80‑1 (MQ=255)
tCATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAAAATCGCAg < 2:638316/81‑1 (MQ=255)
cATTGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGc > 1:638391/1‑81 (MQ=255)
tGCCATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCg > 2:638390/1‑80 (MQ=255)
cATTTGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACt < 2:638317/81‑1 (MQ=255)
tttGTGCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTa > 2:638377/1‑80 (MQ=255)
gtgCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATg > 1:638383/1‑81 (MQ=255)
tgCCATTGTGATGAGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATg > 2:638386/1‑80 (MQ=255)
|
GGAGCATTCTGGTCTGGGCGGTATTGGCGTGATGATTATTATGGCGCTGGTCATTGCCATTTGTGCCATTGTGATGGGCTCTGGCAATGCGCCGTTTATGTCATTTGCCAGTCTTATTCCGAATATCGCAGCCGGACTACATG > NC_000913/3375897‑3376039
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
