Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,411,849 |
T→C |
F66S (TTT→TCT) |
yhdJ → |
DNA adenine methyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,411,849 | 0 | T | C | 100.0%
| 94.7
/ NA
| 28 | F66S (TTT→TCT) | yhdJ | DNA adenine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/15); total (13/15) |
CCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGC > NC_000913/3411772‑3411926
|
cccACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGa < 2:647259/81‑1 (MQ=255)
ccaccaTATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTg > 2:647294/1‑79 (MQ=255)
caTATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGa > 2:647313/1‑81 (MQ=255)
aCATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTg > 1:647308/1‑78 (MQ=255)
aTCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCagag < 1:647260/81‑1 (MQ=255)
aTCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCagag > 2:647309/1‑81 (MQ=255)
aaaaTTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCAc < 2:647261/80‑1 (MQ=255)
aaTTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACcgc < 1:647262/81‑1 (MQ=255)
aTGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTg > 2:647299/1‑80 (MQ=255)
aTGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTg < 2:647263/80‑1 (MQ=255)
gTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGaaaa < 1:647264/81‑1 (MQ=255)
gAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAggg > 1:647306/1‑80 (MQ=255)
gAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgc < 1:647265/81‑1 (MQ=255)
gCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgcagc < 2:647266/81‑1 (MQ=255)
gCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGgcag > 2:647298/1‑80 (MQ=255)
cTGGAAAGAAGATCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCAt < 2:647267/81‑1 (MQ=255)
gaagaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAcatc < 2:647268/81‑1 (MQ=255)
gaagaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAcat > 1:647303/1‑80 (MQ=255)
gaagaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTAcat > 2:647296/1‑80 (MQ=255)
gaTCTGTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCGGGGCAGCATGTACATCATg < 2:647269/81‑1 (MQ=255)
gTTTATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAg > 2:647304/1‑81 (MQ=255)
tttATCGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGa < 2:647270/76‑1 (MQ=255)
cGACTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACgg > 1:647307/1‑80 (MQ=255)
aCTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGaaa < 1:647271/81‑1 (MQ=255)
aCTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGaa > 1:647295/1‑80 (MQ=255)
cTGGCTGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGaaaa < 2:647272/81‑1 (MQ=255)
cTGTCTGAAATTATTGCAGAGTGCCACCTCATTCTTACAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATg < 1:1039640/81‑1 (MQ=255)
tGTCTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGc > 2:647302/1‑81 (MQ=255)
|
CCCACCATATAACATCGGTAAAAATTTTGATGGTCTGATCGAAGCCTGGAAAGAAGATCTGTTTATCGACTGGCTGTTTGAAGTGATTGCAGAGTGCCACCGCGTTCTGAAAAAGCAGGGCAGCATGTACATCATGAACAGTACGGAAAACATGC > NC_000913/3411772‑3411926
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
