Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
182 |
31.9 |
1789978 |
98.5% |
1763128 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,243,047 |
A→T |
Y90N (TAT→AAT) |
uxaA ← |
D‑altronate dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,243,047 | 0 | A | T | 100.0%
| 171.5
/ NA
| 45 | Y90N (TAT→AAT) | uxaA | D‑altronate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (24/21); total (24/21) |
TAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGC > NC_000913/3242972‑3243116
|
tAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGAt > 1:558727/1‑81 (MQ=255)
aGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc > 1:558740/1‑81 (MQ=255)
aGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc > 2:558731/1‑81 (MQ=255)
gcgcGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATcc < 2:558680/81‑1 (MQ=255)
gcgcGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc < 1:558681/80‑1 (MQ=255)
cgcgACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCa < 2:558682/81‑1 (MQ=255)
gacgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATc > 2:558737/1‑76 (MQ=255)
gacgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGAt < 1:558683/81‑1 (MQ=255)
cgaTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCg > 2:558723/1‑81 (MQ=255)
aTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt > 1:558724/1‑81 (MQ=255)
aTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt < 1:558685/81‑1 (MQ=255)
aTAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt < 1:558684/81‑1 (MQ=255)
aTCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCt > 1:558728/1‑77 (MQ=255)
gCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCg > 1:558736/1‑81 (MQ=255)
cACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGAtt < 1:558686/78‑1 (MQ=255)
ttCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTgcg > 1:558726/1‑81 (MQ=255)
cACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGcgcg > 2:558741/1‑81 (MQ=255)
gATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTAt > 2:558729/1‑81 (MQ=255)
aTCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTAtt > 2:558763/1‑81 (MQ=255)
tGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATtgtg > 1:558734/1‑81 (MQ=255)
ccGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTgg < 2:558687/80‑1 (MQ=255)
cGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGc < 1:558688/81‑1 (MQ=255)
cGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGc < 2:558689/81‑1 (MQ=255)
gCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcg > 1:558749/1‑81 (MQ=255)
cTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgt > 2:558750/1‑81 (MQ=255)
cTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgt < 2:558690/81‑1 (MQ=255)
gcgcAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaa > 1:558733/1‑81 (MQ=255)
gcgcAGGCAGATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtg < 2:558691/79‑1 (MQ=255)
gcaggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacg > 2:558746/1‑81 (MQ=255)
aggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgt > 1:558739/1‑80 (MQ=255)
ggcagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGcgtgaacgtga < 2:558692/81‑1 (MQ=255)
cagATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCgtgaacgtgaac < 1:558693/81‑1 (MQ=255)
agATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAaca > 1:558745/1‑81 (MQ=255)
gATCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAacac < 2:558694/81‑1 (MQ=255)
tCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCt < 2:558697/81‑1 (MQ=255)
tCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCt < 1:558696/81‑1 (MQ=255)
tCCTGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCt < 1:558695/81‑1 (MQ=255)
tGAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGcc > 1:558753/1‑81 (MQ=255)
gAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCg > 2:558735/1‑81 (MQ=255)
gAAAATCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCg > 1:558747/1‑81 (MQ=255)
aaaaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGcc < 2:558698/79‑1 (MQ=255)
aaaaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCg > 1:558744/1‑80 (MQ=255)
aaaaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGc < 1:558699/81‑1 (MQ=255)
aaTCAGGTTGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCaa < 1:558700/81‑1 (MQ=255)
ttGATAGCGATTCTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGc > 1:558760/1‑81 (MQ=255)
|
TAGCGCGACGATAGATCTGCACTTCACGATCTGCCGCTTGCGCAGGCAGATCCTGAAAATCAGGTTGATAGCGATACTGATCCAGATCGCTCAGATTCGTGCGCGTATTGTGGGCGTGAACGTGAACACCTGCCGCAATATCCGC > NC_000913/3242972‑3243116
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
