Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
182 |
31.9 |
1789978 |
98.5% |
1763128 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,447,858 |
G→T |
R98S (CGT→AGT) |
rplN ← |
50S ribosomal subunit protein L14 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,447,858 | 0 | G | T | 100.0%
| 113.1
/ NA
| 30 | R98S (CGT→AGT) | rplN | 50S ribosomal subunit protein L14 |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/16); total (14/16) |
TCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTC > NC_000913/3447788‑3447933
|
tCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGa > 1:603865/1‑79 (MQ=255)
cTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATa < 2:603820/80‑1 (MQ=255)
gTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGgct > 1:603852/1‑81 (MQ=255)
cAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTc < 2:603821/81‑1 (MQ=255)
cAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTc > 1:603848/1‑81 (MQ=255)
agagaTAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCt < 2:603823/81‑1 (MQ=255)
agagaTAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCt < 2:603822/81‑1 (MQ=255)
tAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCtgttgt < 1:603825/81‑1 (MQ=255)
tAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCtgttgt < 1:603824/81‑1 (MQ=255)
tCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTgttgttgtt > 1:603877/1‑79 (MQ=255)
tCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTc < 2:603826/80‑1 (MQ=255)
cATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTgttgttgtt > 2:603851/1‑78 (MQ=255)
cATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCa < 1:603827/80‑1 (MQ=255)
aTGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCaga < 2:603828/81‑1 (MQ=255)
aCTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAgaagaa < 2:603829/81‑1 (MQ=255)
aCTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAg > 2:603871/1‑80 (MQ=255)
aCTACGAAGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAg > 2:603870/1‑80 (MQ=255)
aaGCTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTAcc < 1:603830/81‑1 (MQ=255)
cTCACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATc > 1:603863/1‑81 (MQ=255)
cACGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCg > 2:603868/1‑80 (MQ=255)
aCGAGTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCg > 2:603854/1‑79 (MQ=255)
gTTACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGa < 2:603831/81‑1 (MQ=255)
ttACCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGaa < 2:603832/81‑1 (MQ=255)
aCCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAAt < 1:603833/80‑1 (MQ=255)
aCCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAAt < 1:603834/80‑1 (MQ=255)
aCCGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAAt > 1:603859/1‑80 (MQ=255)
ccGGCCCAAAAATACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGa < 2:603835/81‑1 (MQ=255)
cAAAAATACTCGTACCGATAGGCTGCTC‑‑‑GATGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGt > 2:603855/1‑81 (MQ=255)
aaTACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTc > 2:603862/1‑81 (MQ=255)
aaTACTCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTc > 2:603864/1‑81 (MQ=255)
|
TCTGGTGCCAGAGAGATAATTTTCATGAACTTCTCACTACGAAGCTCACGAGTTACCGGCCCAAAAATACGCGTACCGATAGGCTGCTCGCTGTTGTTGTTCAGAAGAACACAAGCATTACCATCGAAGCGAATGACAGAACCGTC > NC_000913/3447788‑3447933
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
