Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
182 |
31.9 |
1789978 |
98.5% |
1763128 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,471,278 |
T→A |
D57V (GAT→GTT) |
pyrI ← |
aspartate carbamoyltransferase, PyrI subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,471,278 | 0 | T | A | 100.0%
| 132.6
/ NA
| 36 | D57V (GAT→GTT) | pyrI | aspartate carbamoyltransferase, PyrI subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (16/20); total (16/20) |
CCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGAA > NC_000913/4471201‑4471351
|
ccGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACtt < 2:848126/81‑1 (MQ=255)
cGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACttt < 1:848128/81‑1 (MQ=255)
cGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACttt < 1:848127/81‑1 (MQ=255)
tGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGc > 1:848170/1‑81 (MQ=255)
gCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCgg > 2:848164/1‑81 (MQ=255)
gCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCgg < 2:848129/81‑1 (MQ=255)
gcggcgCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCa > 1:848162/1‑81 (MQ=255)
cggcgCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCa < 2:848130/80‑1 (MQ=255)
cggcgCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCAt < 1:848131/81‑1 (MQ=255)
gcgcATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCAtct > 1:848171/1‑81 (MQ=255)
cAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAg > 1:848175/1‑80 (MQ=255)
aTGCCAGTTGATCTACTTGATCTTCACTTAAAAAGGCATGTTAGATTCTGATCAGAACTTTGCTGCCCATCTCGCCAGGAg > 1:891215/1‑81 (MQ=255)
ccAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGc < 1:848132/80‑1 (MQ=255)
gATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGtt > 2:848185/1‑79 (MQ=255)
cTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAg < 2:848133/76‑1 (MQ=255)
cTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGAcc < 2:848134/79‑1 (MQ=255)
ttGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAAt > 2:848169/1‑81 (MQ=255)
gATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATg < 2:848135/80‑1 (MQ=255)
cACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGa < 1:848136/78‑1 (MQ=255)
cACTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATgc > 1:848180/1‑81 (MQ=255)
aCTCAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATgcg < 2:848137/81‑1 (MQ=255)
cAAAAAGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCt > 1:848174/1‑80 (MQ=255)
aaaaaGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCt < 2:848138/79‑1 (MQ=255)
aaaGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATc > 2:848166/1‑81 (MQ=255)
aGGTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCg < 2:848139/81‑1 (MQ=255)
gTATTTTCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGtt < 1:848140/81‑1 (MQ=255)
tCGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGt < 1:848141/80‑1 (MQ=255)
cGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGt > 1:848172/1‑79 (MQ=255)
cGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTc < 2:848143/80‑1 (MQ=255)
cGATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTc < 1:848142/80‑1 (MQ=255)
gATTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTCa < 1:848144/80‑1 (MQ=255)
aTTTTGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTCAGc > 1:848191/1‑81 (MQ=255)
tGATCAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTCAGCTTg < 1:848145/80‑1 (MQ=255)
cAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGaa > 1:848193/1‑78 (MQ=255)
cAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGaa > 1:848190/1‑78 (MQ=255)
cAGAACTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGaa > 2:848194/1‑78 (MQ=255)
|
CCGTGGCTTGCGGCGCATACAATGCCAGTTGATCTACTTGATCTTCACTCAAAAAGGTATTTTCGATTTTGATCAGATCTTTGCGGCCCATCTCGCCAGAAGGCAGGTTCAGACCAATGGTGATGCGCTGATCCGTTTCGGTCAGCTTGAA > NC_000913/4471201‑4471351
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
