Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,902,559 |
G→A |
D222N (GAT→AAT) |
ygcE → |
putative sugar kinase YgcE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,902,559 | 0 | G | A | 100.0%
| 108.3
/ NA
| 31 | D222N (GAT→AAT) | ygcE | putative sugar kinase YgcE |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (21/10); total (21/10) |
TCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTA > NC_000913/2902485‑2902636
|
tCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGaaa > 2:472105/1‑81 (MQ=255)
ccGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGCGGGCCGGATTACTGGATAATGAAAc < 2:472061/80‑1 (MQ=255)
ggCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAAcggc > 2:472100/1‑81 (MQ=255)
ggCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAAcggc > 2:472113/1‑81 (MQ=255)
gCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAAcggcg > 2:472122/1‑81 (MQ=255)
gCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAAcggcg > 1:472115/1‑81 (MQ=255)
ccGGTTGTTTGTACCACCAGTGATAATCCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGGAACGGCGGTAATTtc < 2:472062/80‑1 (MQ=255)
cGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTtctc > 2:472103/1‑81 (MQ=255)
cGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTtctc > 2:472097/1‑81 (MQ=255)
cGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTtctc < 1:472063/81‑1 (MQ=255)
ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTctct > 1:472099/1‑81 (MQ=255)
ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTctct > 1:472112/1‑81 (MQ=255)
ggTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTctct < 2:472064/81‑1 (MQ=255)
ttgtttgtACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTg < 1:472065/81‑1 (MQ=255)
tACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTa > 2:472108/1‑81 (MQ=255)
accaccAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTa > 1:472117/1‑80 (MQ=255)
accaccAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTa > 2:472107/1‑80 (MQ=255)
caGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCg > 1:472106/1‑80 (MQ=255)
aGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCa < 1:472066/81‑1 (MQ=255)
gATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAtt > 2:472110/1‑80 (MQ=255)
aaCCGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatg < 1:472067/80‑1 (MQ=255)
ccGGTAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatgat > 2:472109/1‑80 (MQ=255)
tAGAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACg < 2:472068/81‑1 (MQ=255)
gAAGCTCTGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGc < 1:472069/81‑1 (MQ=255)
ctGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCa > 2:472114/1‑81 (MQ=255)
tGGGGGCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGc < 2:472070/79‑1 (MQ=255)
gggggCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACt > 2:472119/1‑81 (MQ=255)
gggCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGc > 2:472126/1‑81 (MQ=255)
ggCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGcc > 2:472120/1‑81 (MQ=255)
gCCGGATTACTGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGcc > 1:472118/1‑80 (MQ=255)
tGGATAATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAg > 1:472111/1‑81 (MQ=255)
gataatGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTa < 2:472071/81‑1 (MQ=255)
|
TCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTA > NC_000913/2902485‑2902636
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
