Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,918,157 |
Δ1 bp |
coding (1229/1341 nt) |
gudD ← |
D‑glucarate dehydratase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,918,155 | 0 | G | . | 100.0%
| 94.2
/ NA
| 23 | coding (1231/1341 nt) | gudD | D‑glucarate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (11/12); total (11/12) |
CCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCTTT > NC_000913/2918081‑2918230
|
ccagccagGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATggcttt > 1:475099/1‑76 (MQ=255)
gccagGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTtcatc < 2:475075/81‑1 (MQ=255)
gccagGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTtcat > 1:475120/1‑80 (MQ=255)
agGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACt > 2:475115/1‑81 (MQ=255)
ggAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACtt < 2:475076/81‑1 (MQ=255)
cAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATc > 1:475107/1‑80 (MQ=255)
aGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATcc < 1:475077/80‑1 (MQ=255)
tGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGa > 2:475110/1‑81 (MQ=255)
cATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGa < 2:475080/79‑1 (MQ=255)
cATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGAtt < 2:475078/81‑1 (MQ=255)
cATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGAt < 2:475079/80‑1 (MQ=255)
ttCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTc > 2:475111/1‑81 (MQ=255)
atcgcatcgTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTacac < 1:475081/81‑1 (MQ=255)
atcgTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGc < 1:475082/81‑1 (MQ=255)
cACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCgg > 2:475109/1‑81 (MQ=255)
gcgcCAAGCCCGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGttttt > 1:475112/1‑81 (MQ=255)
ccGTGTTTCTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCt < 1:475083/81‑1 (MQ=255)
cTGATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGc > 1:475119/1‑81 (MQ=255)
gATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGccc > 1:475124/1‑81 (MQ=255)
gATACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGccc > 1:475117/1‑81 (MQ=255)
tACAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGc < 1:475084/81‑1 (MQ=255)
aCAGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGcc > 1:475106/1‑81 (MQ=255)
aGCTCAT‑GGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCtt < 1:475085/81‑1 (MQ=255)
gCTCAT‑GGCTTTCATCACTTGATCCATATCGAATTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCttt < 2:475086/81‑1 (MQ=255)
|
CCAGCCAGGAATCAGATACTGCATTCCCATCGCATCGTCACGCGCGCCAAGCCCGTGTTTCTGATACAGCTCATGGGCTTTCATCACTTGATCCATATCGATTTCTACACCCAGCCCCGGTTTTTCTGGCACCTGTACCAGCCCGCCTTT > NC_000913/2918081‑2918230
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
