Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,922,977 |
T→C |
E3G (GAA→GGA) |
yqcA ← |
putative flavodoxin YqcA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,922,977 | 0 | T | C | 100.0%
| 75.3
/ NA
| 23 | E3G (GAA→GGA) | yqcA | putative flavodoxin YqcA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/14); total (9/14) |
GCGGTCAGAATCGCTTCCGCTTCTTCGGCCACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACCAATTTCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGACCCGATGGCGCACTGAACTCAACCCTT > NC_000913/2922902‑2923053
|
gcgGTCAGAATCGCTTCCGCTTCTTCGGCCACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGcc < 1:476096/81‑1 (MQ=255)
ggTCAGAATCGCTTCCGCTTCTTCGGCCACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCAt < 2:476098/81‑1 (MQ=255)
ggTCAGAATCGCTTCCGCTTCTTCGGCCACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCAt < 2:476097/81‑1 (MQ=255)
tCGCTTCCGCTTCTTCGGCCACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGtttt < 1:476099/81‑1 (MQ=255)
gcttccgcttcTTCGGCCACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTtc < 2:476100/80‑1 (MQ=255)
ccgcttcTTCGGCCACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACAAATTCCCGCCATACCGTTTTCTccc < 2:476101/80‑1 (MQ=255)
cttcGGCCATTAACCGTGATTTCCCGCCCATGGTGCAGACAAAAAAACCATTCCCCGCCATACCGTTTTCTCCCTGGAt < 2:476102/79‑1 (MQ=255)
gCCACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAAc > 1:476135/1‑81 (MQ=255)
cACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACt > 2:476148/1‑80 (MQ=255)
tGAATTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCa < 2:476103/81‑1 (MQ=255)
aaTTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATc > 2:476138/1‑81 (MQ=255)
aTTCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATcc > 2:476144/1‑81 (MQ=255)
ttCCCGTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCt < 2:476104/81‑1 (MQ=255)
ttCCCGTACATGGTGCCGACAAAAATACAAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCt < 1:476105/81‑1 (MQ=255)
cGTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGAcc > 2:476140/1‑81 (MQ=255)
gTACATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGAccc > 1:476134/1‑81 (MQ=255)
cATGGTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGACCCGAt > 2:476146/1‑81 (MQ=255)
gTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGACCCGATGgcg > 2:476133/1‑81 (MQ=255)
gTGCCGACAAAAATACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGACCCGATGgc < 2:476106/80‑1 (MQ=255)
aaaaaTACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGACCCGATGGCGCACTGAAc < 1:476107/81‑1 (MQ=255)
aaaTACCAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGACCCGATGGCGCACTGAACTc < 2:476108/81‑1 (MQ=255)
cAATTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGACCCGATGGCGCACTGAACTCAACCCt < 1:476109/81‑1 (MQ=255)
aaTTCCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGACCCGATGGCGCACTGAACTCAACCCtt > 2:476151/1‑81 (MQ=255)
|
GCGGTCAGAATCGCTTCCGCTTCTTCGGCCACTAACAGTGAATTCCCGTACATGGTGCCGACAAAAATACCAATTTCCGCCATACCGTTTTCTCCCTGGATTAGGAACTTATCTCTCCATCCTGACCCGATGGCGCACTGAACTCAACCCTT > NC_000913/2922902‑2923053
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
