Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,257,401 |
G→T |
H204N (CAT→AAT) |
cyuP ← |
putative D/L‑serine transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,257,401 | 0 | G | T | 100.0%
| 150.6
/ NA
| 42 | H204N (CAT→AAT) | cyuP | putative D/L‑serine transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (20/22); total (20/22) |
TAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTGA > NC_000913/3257329‑3257478
|
tAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTc > 1:547174/1‑81 (MQ=255)
aaCGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTccc < 2:547109/81‑1 (MQ=255)
aaCGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTcc > 2:547169/1‑80 (MQ=255)
aaCGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTcc > 1:547162/1‑80 (MQ=255)
cGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCccac < 1:547112/81‑1 (MQ=255)
cGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCccac < 2:547110/81‑1 (MQ=255)
aGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATc > 2:547158/1‑81 (MQ=255)
aGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATc > 2:547143/1‑81 (MQ=255)
gCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATc > 2:547154/1‑80 (MQ=255)
gCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCg < 2:547112/81‑1 (MQ=255)
taataaTGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGaca > 2:547162/1‑81 (MQ=255)
taataaTGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGaca > 1:547160/1‑81 (MQ=255)
gCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAgc < 2:547113/81‑1 (MQ=255)
tttCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGcgccg < 2:547114/80‑1 (MQ=255)
cACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGac > 2:547164/1‑81 (MQ=255)
agcCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACcag > 2:547176/1‑80 (MQ=255)
agcCCCAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACcag > 2:547178/1‑80 (MQ=255)
cAGCGGCGGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCtt < 1:547116/81‑1 (MQ=255)
gcggcgGTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTgg > 1:547185/1‑81 (MQ=255)
ggcggTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGt > 1:547164/1‑80 (MQ=255)
ggcggTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGt > 1:547161/1‑80 (MQ=255)
cggTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATTGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAg > 1:547178/1‑81 (MQ=255)
ggTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGc < 2:547116/81‑1 (MQ=255)
gTAGCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCa < 1:547118/81‑1 (MQ=255)
gCGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCa < 1:547119/81‑1 (MQ=255)
cGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCAt < 1:547120/81‑1 (MQ=255)
cGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCAt < 1:547121/81‑1 (MQ=255)
cGAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCAt < 1:547122/81‑1 (MQ=255)
gAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATg > 2:547182/1‑81 (MQ=255)
gAACCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATg > 1:547168/1‑81 (MQ=255)
aCCGACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGcc > 2:547180/1‑81 (MQ=255)
gACGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGt < 1:547123/81‑1 (MQ=255)
aCGTTATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTc > 2:547181/1‑81 (MQ=255)
ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaa < 1:547126/81‑1 (MQ=255)
ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaa < 1:547124/81‑1 (MQ=255)
ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaa < 2:547124/81‑1 (MQ=255)
ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGa > 2:547174/1‑80 (MQ=255)
ttATACAGATTCCACATTCCCACCATCGACACGCCCAGCACCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGaa < 1:547127/81‑1 (MQ=255)
taCAGATTCCACATACCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAtt < 1:547128/81‑1 (MQ=255)
cAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAtttt < 2:547128/81‑1 (MQ=255)
cAGATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAAttt < 2:547129/80‑1 (MQ=255)
gATTCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTGa < 2:547130/81‑1 (MQ=255)
|
TAAACGGCAGCGTAATAATGGCGTTTTTCACCAGCAGCCCCAGCGGCGGTAGCGAACCGACGTTATACAGATGCCACATTCCCACCATCGACACGCCCAGCGCCGCGACCACCAGCAGCTTGGTCAGCACCATGCCGGTCGAAATTTTGA > NC_000913/3257329‑3257478
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
