Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,310,694 |
C→T |
D159N (GAC→AAC) |
pnp ← |
polynucleotide phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,310,694 | 0 | C | T | 100.0%
| 116.1
/ NA
| 32 | D159N (GAC→AAC) | pnp | polynucleotide phosphorylase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/16); total (16/16) |
CTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGC > NC_000913/3310619‑3310769
|
cTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGa < 2:558950/81‑1 (MQ=255)
aGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGt > 2:559001/1‑80 (MQ=255)
ccGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAAcc < 1:558952/80‑1 (MQ=255)
aacaacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTAcgcg < 2:558952/81‑1 (MQ=255)
aacCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGgc < 1:558954/81‑1 (MQ=255)
cAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGgcagc < 2:558954/81‑1 (MQ=255)
aTCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCAc > 1:558999/1‑80 (MQ=255)
tCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCAcc > 1:559016/1‑80 (MQ=255)
aGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATc < 1:558956/81‑1 (MQ=255)
tGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGc > 1:559018/1‑81 (MQ=255)
tttCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCAtt < 2:558956/78‑1 (MQ=255)
tCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGa < 1:558958/78‑1 (MQ=255)
cAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACg > 1:559003/1‑80 (MQ=255)
cGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAc > 2:559021/1‑81 (MQ=255)
cGTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAc > 2:559016/1‑81 (MQ=255)
gTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAcc < 2:558958/81‑1 (MQ=255)
gTCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAc > 1:559010/1‑80 (MQ=255)
tCCTGAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATAc > 1:559026/1‑79 (MQ=255)
gAGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACcaga > 2:559008/1‑79 (MQ=255)
aGTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACcagacag < 2:558959/81‑1 (MQ=255)
gTCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACcagaca > 1:559014/1‑79 (MQ=255)
tCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCagacagag < 2:558960/81‑1 (MQ=255)
tCGGGTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCagacagag < 2:558961/81‑1 (MQ=255)
ggTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCagacagagaca < 1:558963/81‑1 (MQ=255)
gTTCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCagacagagacag > 1:559013/1‑81 (MQ=255)
ttCAGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAgc > 1:559011/1‑81 (MQ=255)
aGTACGTACTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCt > 2:559024/1‑81 (MQ=255)
gtacgtacTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTg > 2:559020/1‑81 (MQ=255)
tacgtacTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGc < 1:558964/81‑1 (MQ=255)
tacgtacTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGc > 1:559012/1‑81 (MQ=255)
cTGGTTATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGc < 2:558965/81‑1 (MQ=255)
cTGGTTATTGATGTAACCTACGCGGGCAGAACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAg < 2:558964/80‑1 (MQ=255)
|
CTTCAGTACCGGCAACAACCAGATCCAGTTTGCTCTCTTTCAGCTCGTCCTGAGTCGGGTTCAGTACGTACTGGTCATTGATGTAACCTACGCGGGCAGCACCAATCGGGCCATTGAACGGAATACCAGACAGAGACAGCGCTGCGGAAGC > NC_000913/3310619‑3310769
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
