Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,324,584 |
C→A |
G110C (GGC→TGC) |
folP ← |
dihydropteroate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,324,584 | 0 | C | A | 100.0%
| 117.9
/ NA
| 33 | G110C (GGC→TGC) | folP | dihydropteroate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (17/16); total (17/16) |
CCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGCAATTG > NC_000913/3324506‑3324661
|
ccggtaaaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCaaa < 1:562237/81‑1 (MQ=255)
ggtaaaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACt > 1:562280/1‑81 (MQ=255)
gtgaaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACtt < 2:562237/78‑1 (MQ=255)
aaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTc < 1:562239/80‑1 (MQ=255)
aaccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCg < 1:562240/81‑1 (MQ=255)
ccggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCt < 1:562241/81‑1 (MQ=255)
cggtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCt > 1:562242/1‑80 (MQ=255)
gtTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATTATTAATAATGTGAGCGCAAACTTTCGCTGAc > 2:562289/1‑81 (MQ=255)
cTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCAc > 2:562288/1‑81 (MQ=255)
cgccTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGAc < 2:562242/81‑1 (MQ=255)
gccTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACt < 2:562243/81‑1 (MQ=255)
ccTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACtt > 1:562284/1‑81 (MQ=255)
agagCGCCCGGTTCGGATAGGGAGCGGATATCATCAACAATGTGAGCGAAAACTTTCGCTGACTCATGGATGACTACTGGt > 2:562295/1‑81 (MQ=255)
gTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTAt > 1:562306/1‑81 (MQ=255)
ttCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATc > 1:562323/1‑81 (MQ=255)
ttCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATc < 2:562244/81‑1 (MQ=255)
cGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCg < 2:562245/80‑1 (MQ=255)
ggAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGa < 2:562246/80‑1 (MQ=255)
gAAAGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACt > 1:562298/1‑81 (MQ=255)
aaGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACt > 2:562308/1‑79 (MQ=255)
aaGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACt > 2:562287/1‑79 (MQ=255)
aaGGGAGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTga < 1:562248/81‑1 (MQ=255)
aGCGGATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATc > 2:562296/1‑80 (MQ=255)
ggATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGa > 1:562304/1‑81 (MQ=255)
ggATATCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGa > 1:562295/1‑81 (MQ=255)
atCATTAATAATGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTc > 1:562294/1‑81 (MQ=255)
tCATTAATAATGTGAGCGAAAACTTTCGCTGACTAACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCg < 1:562249/81‑1 (MQ=255)
aataatGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGc < 2:562249/80‑1 (MQ=255)
ataatGTGAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGt < 2:562250/81‑1 (MQ=255)
tgAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGc < 2:562251/80‑1 (MQ=255)
tgAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGc < 2:562252/80‑1 (MQ=255)
gAGCGCAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGTGATCCAGACTTCGAAGCGTTGAGCaa > 1:562332/1‑81 (MQ=255)
gcgcAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGCAAtt > 1:562317/1‑81 (MQ=255)
cgcAAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGCAATTg < 1:562254/81‑1 (MQ=255)
|
CCGGTAAACCGGTTTCTGCAGCCGCCTCCAGAGCGCCAGGTTCGGAAAGGGAGCGGATATCATTAATAATGTGAGCGCCAACTTTCGCTGACTCACGGATGACTTCTGGTTTGGATGTATCGACTGAGATCCAGACTTCGAAGCGTTGAGCAATTG > NC_000913/3324506‑3324661
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
