Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,328,268 |
A→G |
F150L (TTT→CTT) |
greA ← |
transcription elongation factor GreA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,328,268 | 0 | A | G | 100.0%
| 77.9
/ NA
| 23 | F150L (TTT→CTT) | greA | transcription elongation factor GreA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/11); total (12/11) |
TTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATA > NC_000913/3328193‑3328341
|
tttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTc < 2:563147/79‑1 (MQ=255)
tttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTa > 1:563188/1‑81 (MQ=255)
ccTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACtt > 2:563183/1‑81 (MQ=255)
ctttctttACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTc > 1:563179/1‑81 (MQ=255)
ctttACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTcgccg > 1:563181/1‑81 (MQ=255)
accaTACATCAACATCTTGAGTATTGGGTAATTCTTTCAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGccc < 2:563148/77‑1 (MQ=255)
aCAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGccc < 2:563149/80‑1 (MQ=255)
aTACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCg > 1:563187/1‑81 (MQ=255)
cATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGtt < 2:563150/80‑1 (MQ=255)
aTCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGtttt < 2:563151/81‑1 (MQ=255)
cTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACca > 2:563182/1‑81 (MQ=255)
gAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACcaca < 2:563152/80‑1 (MQ=255)
gTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCacaaca > 1:563190/1‑81 (MQ=255)
tAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttc < 1:563154/81‑1 (MQ=255)
aaTTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCAtcttct > 2:563184/1‑81 (MQ=255)
tACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTg > 1:563191/1‑81 (MQ=255)
aGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGcc > 1:563194/1‑80 (MQ=255)
ggTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGa < 1:563155/81‑1 (MQ=255)
gTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt > 1:563192/1‑81 (MQ=255)
tATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATc < 1:563157/81‑1 (MQ=255)
tATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATc < 1:563156/81‑1 (MQ=255)
aTTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt > 1:563193/1‑79 (MQ=255)
cTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATa < 1:563158/80‑1 (MQ=255)
|
TTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATA > NC_000913/3328193‑3328341
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
