Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
730 |
35.1 |
2107516 |
91.4% |
1926269 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,328,268 |
A→G |
F150L (TTT→CTT) |
greA ← |
transcription elongation factor GreA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,328,268 | 0 | A | G | 100.0%
| 75.9
/ NA
| 23 | F150L (TTT→CTT) | greA | transcription elongation factor GreA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/10); total (13/10) |
TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAGGC > NC_000913/3328192‑3328344
|
ttttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTc > 1:626435/1‑80 (MQ=255)
ttttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTc > 1:626452/1‑80 (MQ=255)
ttttCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTc > 2:626446/1‑80 (MQ=255)
ctttctttACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACtt < 1:626421/80‑1 (MQ=255)
tACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGccc > 2:626474/1‑81 (MQ=255)
tACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGt < 1:626422/81‑1 (MQ=255)
aTCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGtttt > 1:626457/1‑81 (MQ=255)
aGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCacaac < 1:626423/81‑1 (MQ=255)
aTTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAAcat > 2:626453/1‑80 (MQ=255)
aTTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAAcat > 2:626451/1‑80 (MQ=255)
tGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTTATGACCACAAcatcat > 2:626448/1‑81 (MQ=255)
gTATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt < 1:626424/81‑1 (MQ=255)
tATTCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATc < 2:626425/81‑1 (MQ=255)
aTTCCACCTTAATTGCTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGAt > 2:626454/1‑79 (MQ=255)
tCCACCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAgg > 2:626477/1‑81 (MQ=255)
aCCTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCa > 1:626455/1‑81 (MQ=255)
ccTTAATTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCAc < 2:626426/81‑1 (MQ=255)
aaTTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGa < 1:626427/79‑1 (MQ=255)
aTTACTTCAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCa < 2:626428/81‑1 (MQ=255)
cTTCAAGTTCCACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAg > 1:626465/1‑81 (MQ=255)
cTTCAAGTTCCACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAg > 1:626463/1‑81 (MQ=255)
cAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAGGc < 2:626429/80‑1 (MQ=255)
cAAGTTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAGGc < 2:626430/80‑1 (MQ=255)
|
TTTTCCTTTCTTTACAATACATCAACATCTTGAGTATTGGGTAATTCTTACAGGTATTCCACCTTAATTACTTCAAATTCTACTTCGCCGCCCGGCGTTTTGATGACCACAACATCATCTTCTTCTTTGCCGATCAGGCCACGAGCAATAGGC > NC_000913/3328192‑3328344
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
