Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,354,739 |
Δ1 bp |
coding (15/4461 nt) |
gltB → |
glutamate synthase subunit GltB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,354,737 | 0 | A | . | 100.0%
| 129.4
/ NA
| 31 | coding (13/4461 nt) | gltB | glutamate synthase subunit GltB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (15/16); total (15/16) |
TCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCG > NC_000913/3354658‑3354813
|
tctTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATaa > 2:569541/1‑81 (MQ=255)
tgCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCtt < 2:569484/79‑1 (MQ=255)
gCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCtt > 1:569533/1‑78 (MQ=255)
cccGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTggga < 1:569487/81‑1 (MQ=255)
cccGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTgaga < 2:569485/81‑1 (MQ=255)
ccGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGagag < 1:569488/81‑1 (MQ=255)
gTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAgg > 1:569547/1‑80 (MQ=255)
cgcAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGAt < 2:569489/80‑1 (MQ=255)
cgcAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATa < 1:569489/81‑1 (MQ=255)
gcAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATaa > 2:569531/1‑81 (MQ=255)
aGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACt > 2:569536/1‑81 (MQ=255)
aGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACt > 2:569545/1‑81 (MQ=255)
ggTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACtgtg > 2:569539/1‑81 (MQ=255)
ggAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTc > 1:569544/1‑81 (MQ=255)
ggAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATAAGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTc < 2:569490/81‑1 (MQ=255)
tGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCt < 1:569492/81‑1 (MQ=255)
gTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATc > 1:569551/1‑81 (MQ=255)
ccGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGcc < 2:569492/80‑1 (MQ=255)
cGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCca > 2:569548/1‑81 (MQ=255)
gCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCcac < 1:569494/81‑1 (MQ=255)
cAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCcaca > 2:569538/1‑81 (MQ=255)
cAGAGCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCcaca < 1:569495/81‑1 (MQ=255)
agagCCTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCcaca > 2:569556/1‑80 (MQ=255)
cTGGGGGAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAg < 1:569496/81‑1 (MQ=255)
gAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAAc < 1:569498/81‑1 (MQ=255)
gAGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAAc < 2:569496/81‑1 (MQ=255)
aGGTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAAcc < 2:569498/81‑1 (MQ=255)
ggTTCACGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAAcc > 1:569559/1‑80 (MQ=255)
cGATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCcac < 1:569500/81‑1 (MQ=255)
gATATGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCcac > 2:569547/1‑80 (MQ=255)
tatGTTGTACGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAg > 1:569561/1‑81 (MQ=255)
aCGAT‑AATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCg > 2:569559/1‑81 (MQ=255)
|
TCTTTCTGTGCCCGTGCGCAGCGGTTCGGAAGTGGGGTTCCCGCAGAGCCTGGGGGAGGTTCACGATATGTTGTACGATAAATCCCTTGAGAGGGATAACTGTGGTTTCGGCCTGATCGCCCACATAGAAGGCGAACCTAGCCACAAGGTAGTGCG > NC_000913/3354658‑3354813
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
