Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,393,640 |
T→C |
E873E (GAA→GAG) |
yhdP ← |
AsmA2 domain‑containing protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,393,640 | 0 | T | C | 100.0%
| 103.9
/ NA
| 30 | E873E (GAA→GAG) | yhdP | AsmA2 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/18); total (12/18) |
CACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGT > NC_000913/3393564‑3393711
|
caccacCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCa < 2:578407/80‑1 (MQ=255)
accGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGc > 2:578451/1‑80 (MQ=255)
gACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACt < 1:578409/81‑1 (MQ=255)
gACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACt > 1:578475/1‑81 (MQ=255)
cacTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACttt < 1:578411/81‑1 (MQ=255)
cacTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACttt < 2:578409/81‑1 (MQ=255)
ctctCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGt < 1:578412/81‑1 (MQ=255)
tcCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGtt < 1:578413/79‑1 (MQ=255)
cGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTc < 1:578414/78‑1 (MQ=255)
cGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCg < 2:578414/80‑1 (MQ=255)
gcCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAAcgg > 1:578469/1‑81 (MQ=255)
cTTTCTGGAACAGGGCCAGCCACTCGGCACCGTTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAAcggcg < 2:578415/81‑1 (MQ=255)
cTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAAcggcg > 1:578465/1‑81 (MQ=255)
tCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAAcggcgg > 1:578474/1‑79 (MQ=255)
gAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGc < 2:578416/79‑1 (MQ=255)
gAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGt > 1:578463/1‑81 (MQ=255)
aCAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGttt < 2:578417/81‑1 (MQ=255)
aCAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGttt < 2:578418/81‑1 (MQ=255)
gggCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTAc > 2:578476/1‑81 (MQ=255)
ccagccaCTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGt > 2:578419/1‑81 (MQ=255)
caCTCGGCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTgcc < 1:578421/81‑1 (MQ=255)
ggCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTgccgcc < 2:578422/79‑1 (MQ=255)
ggCACCATTCATCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGccc < 1:578422/80‑1 (MQ=255)
tCGGCGGCATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGAt > 1:578471/1‑81 (MQ=255)
tCGGCGGCATATTGAGCTCAACGCCACTTTGATCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGAt < 2:578423/81‑1 (MQ=255)
ggcggcATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATc < 1:578425/80‑1 (MQ=255)
gcggcATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATc > 1:578485/1‑79 (MQ=255)
cggcATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAg > 2:578469/1‑81 (MQ=255)
ggcATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGc > 1:578466/1‑81 (MQ=255)
cATATTGAGCTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGt < 2:578425/81‑1 (MQ=255)
|
CACCACCGACACTCTCCGCAGCGCCTTTCTGGAACAGGGCCAGCCACTCGGCACCATTCATCGGCGGCATATTGAGTTCAACGCCACTTTGTTCCGGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGT > NC_000913/3393564‑3393711
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
