Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,467,731 |
G→A |
A708V (GCA→GTA) |
chiA ← |
endochitinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,467,731 | 0 | G | A | 100.0%
| 125.6
/ NA
| 35 | A708V (GCA→GTA) | chiA | endochitinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/17); total (18/17) |
CAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAAT > NC_000913/3467653‑3467806
|
cAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATAcc < 1:595925/81‑1 (MQ=255)
cAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATAcc > 2:595991/1‑81 (MQ=255)
cAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATAcc > 2:595972/1‑81 (MQ=255)
aGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCt < 2:595925/81‑1 (MQ=255)
aGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCt > 1:595975/1‑81 (MQ=255)
aTAGCAATATCTTTGCCTTCTGACTCCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGa < 1:595927/81‑1 (MQ=255)
aGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATc > 2:595959/1‑81 (MQ=255)
cAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCg > 1:595988/1‑81 (MQ=255)
atatCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGc > 2:595969/1‑80 (MQ=255)
tGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAAccc < 2:595927/78‑1 (MQ=255)
gCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGt > 2:595963/1‑81 (MQ=255)
cTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGc > 2:595966/1‑81 (MQ=255)
cTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCt < 1:595929/80‑1 (MQ=255)
gACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTc > 1:595968/1‑80 (MQ=255)
aCTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTc < 2:595929/79‑1 (MQ=255)
aCTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGa > 1:595976/1‑81 (MQ=255)
cATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATc > 1:595974/1‑80 (MQ=255)
tCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTcc < 1:595931/81‑1 (MQ=255)
tCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTcc < 2:595932/81‑1 (MQ=255)
tCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTc < 2:595931/80‑1 (MQ=255)
cACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGAc < 2:595934/81‑1 (MQ=255)
cACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGAc < 2:595933/81‑1 (MQ=255)
cTTTCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACtt > 2:595981/1‑81 (MQ=255)
tttCTTCACAGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACttt > 2:595979/1‑81 (MQ=255)
cacaGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTACCTTCGCTATCGAAGTCCAGGACTTTGAGGtt > 2:595984/1‑81 (MQ=255)
caGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAAGCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCa < 1:595937/81‑1 (MQ=255)
caGCAAGGTTACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCa < 1:595936/81‑1 (MQ=255)
ttACGACGTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCa < 1:595938/81‑1 (MQ=255)
cgacgTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAAc > 2:595938/1‑80 (MQ=255)
cgTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATg < 2:595939/81‑1 (MQ=255)
gTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGt < 2:595940/81‑1 (MQ=255)
gTTCAATAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGt > 2:595988/1‑81 (MQ=255)
aaTAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCat > 1:595987/1‑80 (MQ=255)
aTAGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCataa < 1:595942/81‑1 (MQ=255)
aGATACCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCataat > 2:595985/1‑80 (MQ=255)
|
CAGATAGCAATATCTTTGCCTTCTGACTTCCATTTATCCTGCACTTTCTTCACAGCAAGGTTACGACGTTCAATAGATGCCTGATCCGCAACCCAGGTGCCTTCGATATCGAAGTCCAGGACTTTGAGGTTCAGGTTATCAACGATGTCATAAT > NC_000913/3467653‑3467806
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
