Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,586,418 |
(T)6→7 |
coding (406/1743 nt) |
ggt ← |
glutathione hydrolase proenzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,586,412 | 1 | . | T | 100.0%
| 108.1
/ NA
| 30 | S138T (TCA→ACA) | ggt | glutathione hydrolase proenzyme |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (8/22); total (8/22) |
GCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑TTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGG > NC_000913/3586338‑3586485
|
gCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑tttttt > 2:625710/1‑81 (MQ=255)
ccGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑ttttttt < 2:625637/81‑2 (MQ=255)
gTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑tttttttgc < 1:625639/81‑4 (MQ=255)
gTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑tttttttgc > 1:625708/1‑78 (MQ=255)
gTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑tttttttgc > 1:625696/1‑78 (MQ=255)
gTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGCGA‑tttttttgc > 1:625700/1‑78 (MQ=255)
tCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTcccg > 2:625697/1‑79 (MQ=255)
tCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCg < 2:625639/80‑1 (MQ=255)
agcgccagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGtt < 1:625641/81‑1 (MQ=255)
cagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCt < 1:625642/81‑1 (MQ=255)
cagcgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCt > 1:625718/1‑81 (MQ=255)
cgAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGat < 2:625642/81‑1 (MQ=255)
ccGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACata < 2:625643/81‑1 (MQ=255)
ccGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACat < 2:625646/80‑1 (MQ=255)
ccGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACat < 2:625644/80‑1 (MQ=255)
ccGTACCCGGTGTGCCGGAAGCCAGATGCAAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACa < 2:625645/79‑1 (MQ=255)
cGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACatat < 1:625648/81‑1 (MQ=255)
gTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATc < 2:625649/81‑1 (MQ=255)
gTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATc < 2:625648/81‑1 (MQ=255)
aCCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATcgc > 2:625735/1‑81 (MQ=255)
aCCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATcgc > 2:625721/1‑81 (MQ=255)
aCCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATcgc > 1:625712/1‑81 (MQ=255)
ggTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGt < 1:625651/81‑1 (MQ=255)
ggTGTGCCGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGt < 2:625651/81‑1 (MQ=255)
ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCttt < 1:625655/81‑1 (MQ=255)
ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCttt < 2:625652/81‑1 (MQ=255)
ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCttt < 2:625653/81‑1 (MQ=255)
ccGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCttt > 2:625702/1‑81 (MQ=255)
cGGAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTg < 1:625656/81‑1 (MQ=255)
gAAGCCAGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGc < 1:625657/81‑1 (MQ=255)
aGATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGc > 1:625714/1‑81 (MQ=255)
gATGCGAAGTGAGTGATTTTTTTGCTGTCCGGGTTGCTCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGcc < 2:625657/81‑1 (MQ=255)
aaGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTcg < 2:625658/81‑1 (MQ=255)
aaGTGAGTGATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTcg > 2:625712/1‑81 (MQ=255)
agtgagtgATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTcgc < 1:625660/81‑1 (MQ=255)
agtgagtgATTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTcgc < 1:625661/81‑1 (MQ=255)
gagtgatTTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCgg > 1:625727/1‑80 (MQ=255)
|
GCCGTATTTATCCAGCGCCAGCGAGAAACCTGCTACCGTACCCGGTGTGCCGGAAGCCAGATGCGAAGTGAGTGA‑TTTTTTGCTGTCCGGGTTGCCCTGATCATCGAGGAACATATCGCGGGTCGCTTTGGCGGGTGCCATTTCGCGG > NC_000913/3586338‑3586485
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
