Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
690 |
31.8 |
1836723 |
97.1% |
1783458 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,419,570 |
T→C |
E19G (GAG→GGG) |
ulaG ← |
L‑ascorbate‑6‑phosphate lactonase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,419,570 | 0 | T | C | 100.0%
| 69.5
/ NA
| 21 | E19G (GAG→GGG) | ulaG | L‑ascorbate‑6‑phosphate lactonase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (4/17); total (4/17) |
CACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCAT > NC_000913/4419501‑4419641
|
caccacATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTg < 2:843025/81‑1 (MQ=255)
gCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATc < 2:843026/81‑1 (MQ=255)
aaaTGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCa < 2:843027/81‑1 (MQ=255)
tgtgCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGa > 2:843070/1‑81 (MQ=255)
tgtgCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGa < 2:843028/81‑1 (MQ=255)
ccAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTc < 2:843029/80‑1 (MQ=255)
aGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACg > 2:843069/1‑81 (MQ=255)
aGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGt < 1:843031/81‑1 (MQ=255)
gACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGAt < 1:843032/81‑1 (MQ=255)
aCCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATa > 2:843066/1‑81 (MQ=255)
cTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACt < 2:843032/81‑1 (MQ=255)
ttCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACtt < 1:843034/79‑1 (MQ=255)
gTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCAc < 2:843034/79‑1 (MQ=255)
ttCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACttt < 1:843036/81‑1 (MQ=255)
tcttcATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCAt < 1:843037/80‑1 (MQ=255)
cattcaACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGc < 2:843037/79‑1 (MQ=255)
cattcaACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCa > 2:843076/1‑81 (MQ=255)
aaCCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTAc < 1:843040/81‑1 (MQ=255)
aaCCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTAc < 2:843038/81‑1 (MQ=255)
cTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCAt < 1:843042/81‑1 (MQ=255)
cTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCAt < 1:843041/81‑1 (MQ=255)
|
CACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCAT > NC_000913/4419501‑4419641
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
