Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,767,394 |
T→C |
H431R (CAC→CGC) |
ydiJ ← |
putative FAD‑linked oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,767,394 | 0 | T | C | 100.0%
| 99.9
/ NA
| 29 | H431R (CAC→CGC) | ydiJ | putative FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/13); total (16/13) |
GACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGTGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAACG > NC_000913/1767318‑1767471
|
gACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTc > 1:304098/1‑81 (MQ=255)
aCGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTcc < 1:304063/81‑1 (MQ=255)
cGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCcg > 2:304104/1‑81 (MQ=255)
gTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCcgg > 2:304102/1‑81 (MQ=255)
tGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCcggc > 2:304107/1‑81 (MQ=255)
ccGAACATACCGTAGCTAAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCcggcgg > 2:304103/1‑80 (MQ=255)
aaCATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACg < 2:304064/81‑1 (MQ=255)
cATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCa < 2:304065/81‑1 (MQ=255)
gTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATc < 1:304067/81‑1 (MQ=255)
gTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATc > 1:304105/1‑81 (MQ=255)
gTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATc < 2:304066/81‑1 (MQ=255)
tAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAg > 1:304101/1‑80 (MQ=255)
aaGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGc < 2:304068/80‑1 (MQ=255)
aaGCCGTGGCTGTCGAGCAGCGCGCGAAAATCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAg < 2:304069/79‑1 (MQ=255)
gTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTgg > 1:304108/1‑81 (MQ=255)
cGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCt > 2:304113/1‑81 (MQ=255)
agcagcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTa < 2:304070/81‑1 (MQ=255)
agcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCg > 2:304117/1‑81 (MQ=255)
gcgcgAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCa > 2:304116/1‑81 (MQ=255)
cgcgAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCAc < 2:304072/81‑1 (MQ=255)
gAAATTCAGCAATAGAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCtt < 2:304073/81‑1 (MQ=255)
aTTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTgg > 2:469697/1‑81 (MQ=255)
tCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCa > 1:304092/1‑81 (MQ=255)
aTCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAg < 1:304074/81‑1 (MQ=255)
cGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGAc < 1:304075/80‑1 (MQ=255)
cAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAc < 1:304076/81‑1 (MQ=255)
cAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAc > 2:304115/1‑81 (MQ=255)
cAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAc > 2:304120/1‑81 (MQ=255)
aGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAACg > 1:304123/1‑81 (MQ=255)
|
GACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGTGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAACG > NC_000913/1767318‑1767471
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
