Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,767,394 |
T→C |
H431R (CAC→CGC) |
ydiJ ← |
putative FAD‑linked oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,767,394 | 0 | T | C | 100.0%
| 97.5
/ NA
| 28 | H431R (CAC→CGC) | ydiJ | putative FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (10/18); total (10/18) |
TCGACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGTGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAC > NC_000913/1767316‑1767470
|
tCGACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGgcgt < 1:325998/81‑1 (MQ=255)
cGACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGtt > 1:326044/1‑81 (MQ=255)
aCGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTcc < 2:325999/81‑1 (MQ=255)
gAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTAc > 1:326046/1‑81 (MQ=255)
aaCATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACg < 2:326002/81‑1 (MQ=255)
aaCATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACg < 2:326001/81‑1 (MQ=255)
aaCATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACg < 2:326000/81‑1 (MQ=255)
cGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTAt > 2:326058/1‑81 (MQ=255)
gTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATc < 2:326003/81‑1 (MQ=255)
cTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCa < 2:326004/81‑1 (MQ=255)
aaGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGc < 1:326005/80‑1 (MQ=255)
gTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACg > 1:326047/1‑80 (MQ=255)
gCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAAt < 1:326006/81‑1 (MQ=255)
cTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAAtt < 2:326007/81‑1 (MQ=255)
gAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCt > 1:326048/1‑80 (MQ=255)
cagcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGTAGGTATCCTCAGCAAACGGAATTGGCTTAGc > 2:326054/1‑81 (MQ=255)
cagcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGc < 2:326008/81‑1 (MQ=255)
agcGCGCGAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCg < 2:326009/81‑1 (MQ=255)
gcgcgAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCa < 1:326010/81‑1 (MQ=255)
cgAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCt < 2:326011/81‑1 (MQ=255)
gAAATTCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCtt > 2:326051/1‑81 (MQ=255)
ttCAGCAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGc < 2:326012/81‑1 (MQ=255)
cAATATAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGc < 2:326013/81‑1 (MQ=255)
atatAATCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCa < 1:326014/81‑1 (MQ=255)
aTCCGCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAg > 2:326057/1‑78 (MQ=255)
gCCAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCa < 2:326015/81‑1 (MQ=255)
ccAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCaa > 1:326053/1‑81 (MQ=255)
ccAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCAACTTTGGCATTGCCAAGCAGACCaa < 2:326016/81‑1 (MQ=255)
cAGGCGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAc > 2:326066/1‑81 (MQ=255)
|
TCGACGTGACCGAACATACCGTAGCTTAAGCCGTGGCTGTCGAGCAGCGCGCGAAATTCAGCAATATAATCCGCCAGGTGTTCCGGCGGTACGCAGGTATCCTCAGCAAACGGAATTGGCTTAGCGGCACCTTTGGCATTGCCAAGCAGACCAAC > NC_000913/1767316‑1767470
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
