Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
524 |
31.4 |
1748232 |
97.8% |
1709770 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,574,860 |
A→T |
C48* (TGT→TGA) |
eutQ ← |
putative ethanolamine utilization protein EutQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,574,860 | 0 | A | T | 100.0%
| 103.9
/ NA
| 30 | C48* (TGT→TGA) | eutQ | putative ethanolamine utilization protein EutQ |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (20/10); total (20/10) |
TCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCAC > NC_000913/2574783‑2574937
|
tCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCAt > 1:439660/1‑81 (MQ=255)
gcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTc > 2:439644/1‑81 (MQ=255)
gcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTc > 2:439643/1‑81 (MQ=255)
cgcTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGAt < 2:439614/80‑1 (MQ=255)
cTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATgg > 2:439658/1‑80 (MQ=255)
gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTaaa < 1:439615/81‑1 (MQ=255)
tCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc < 1:439617/80‑1 (MQ=255)
tCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc < 1:439618/80‑1 (MQ=255)
tCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAc < 2:439616/79‑1 (MQ=255)
cGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc > 2:439663/1‑79 (MQ=255)
gTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGt < 2:439619/81‑1 (MQ=255)
ggCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGc > 1:439655/1‑81 (MQ=255)
ggCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACt > 2:439654/1‑81 (MQ=255)
aCGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCa > 1:439657/1‑81 (MQ=255)
ggCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGc > 2:439661/1‑81 (MQ=255)
ggCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGc > 1:439664/1‑81 (MQ=255)
cGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCtt > 1:439674/1‑81 (MQ=255)
gggCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTc < 1:439620/81‑1 (MQ=255)
gggCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTc < 2:439621/81‑1 (MQ=255)
cACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCgg < 2:439622/81‑1 (MQ=255)
ccGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGtaataa < 2:439623/81‑1 (MQ=255)
tACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCt > 2:439676/1‑81 (MQ=255)
tACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCt > 1:439673/1‑81 (MQ=255)
tACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCt > 1:439670/1‑81 (MQ=255)
gggATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcg > 1:439669/1‑81 (MQ=255)
gATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 1:439666/1‑81 (MQ=255)
tCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:439659/1‑79 (MQ=255)
tCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:439672/1‑79 (MQ=255)
cGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:439656/1‑78 (MQ=255)
cGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:439675/1‑78 (MQ=255)
atctCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCAc > 1:439665/1‑81 (MQ=255)
|
TCGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAACCAC > NC_000913/2574783‑2574937
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
