Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
315 |
33.5 |
1889570 |
98.7% |
1865005 |
80.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,574,860 |
A→T |
C48* (TGT→TGA) |
eutQ ← |
putative ethanolamine utilization protein EutQ |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,574,860 | 0 | A | T | 97.5%
| 139.1
/ NA
| 40 | C48* (TGT→TGA) | eutQ | putative ethanolamine utilization protein EutQ |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (21/18); minor base C (1/0); total (22/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAAC > NC_000913/2574784‑2574934
|
cgcgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCAt < 1:470118/80‑1 (MQ=255)
cgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCg < 1:470119/81‑1 (MQ=255)
cgGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCg > 1:470177/1‑81 (MQ=255)
tGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGa > 2:470179/1‑81 (MQ=255)
cgcTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGAt < 2:470120/80‑1 (MQ=255)
gcTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATgg > 1:470171/1‑81 (MQ=255)
gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTaaa < 1:470121/81‑1 (MQ=255)
gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTaa > 2:470192/1‑80 (MQ=255)
gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTaa < 2:470122/80‑1 (MQ=255)
gCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTa < 1:470123/79‑1 (MQ=255)
tCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc > 1:470183/1‑80 (MQ=255)
cGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAccc < 1:470124/80‑1 (MQ=255)
cGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAAcc > 2:470169/1‑79 (MQ=255)
tGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGa < 1:470125/80‑1 (MQ=255)
gTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCATTATATc > 1:470126/1‑81 (MQ=255)
ggCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGc < 1:470127/81‑1 (MQ=255)
ggCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGc > 1:470187/1‑81 (MQ=255)
ggCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCCCATTCGGTGATGGTAAAACCCAGTAGATCCGc > 1:470195/1‑81 (MQ=255)
gacTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCg < 1:470128/78‑1 (MQ=255)
gCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACtt < 1:470129/81‑1 (MQ=255)
cGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCAc < 2:470130/81‑1 (MQ=255)
gCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACg > 2:470176/1‑81 (MQ=255)
gCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACg > 2:470186/1‑81 (MQ=255)
cTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACgg < 2:470131/81‑1 (MQ=255)
cTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACgg > 2:470189/1‑81 (MQ=255)
gCGGGCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCt < 1:470132/81‑1 (MQ=255)
ggCACGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTcc < 2:470133/81‑1 (MQ=255)
cGGACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCgggg > 2:470193/1‑81 (MQ=255)
gACGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGta < 1:470134/81‑1 (MQ=255)
cGCCGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGtaa > 2:470196/1‑80 (MQ=255)
ccGTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGtaataa < 1:470135/81‑1 (MQ=255)
gTTACCGGGATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATg > 1:470180/1‑81 (MQ=255)
gggATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAGTGCTGgcg > 2:470200/1‑81 (MQ=255)
gATCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:470199/1‑81 (MQ=255)
aTCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:470191/1‑80 (MQ=255)
aTCGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 2:470182/1‑80 (MQ=255)
cGATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGgcgcg > 1:470206/1‑78 (MQ=255)
gATTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGa < 1:470136/81‑1 (MQ=255)
aTTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGaa > 2:470197/1‑81 (MQ=255)
aTTCATCTCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGaa > 1:470190/1‑81 (MQ=255)
tcatctCATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAAc > 2:470185/1‑80 (MQ=255)
|
CGCGGATGCGCTGGCTTTCGGTTTTGTCGGCTGGCACGCTGGCGGGCACGGACGCCGTTACCGGGATCGATTCATCACATTCGGTGATGGTAAAACCCAGTAGATCCGCCACTTCACGGGCTTCCGGGGTAATAATGCTGGCGCGCAGAAC > NC_000913/2574784‑2574934
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
