Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	315	33.5	1889570	98.7%	1865005	80.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,397,596	A→T	F67Y (TTT→TAT)	rng ←	RNase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,397,596	0	A	T	100.0%	127.3 / NA	35	F67Y (TTT→TAT)	rng	RNase G
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (15/20); total (15/20)

GTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGT  >  NC_000913/3397520‑3397671

gTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATAcgc                                                                         >  1:645614/1‑81 (MQ=255)

 tCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATAcgcg                                                                        <  1:645566/81‑1 (MQ=255)

 tCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATAcgc                                                                         <  2:645565/80‑1 (MQ=255)

  cgcgCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCgg                                                                       >  1:645618/1‑81 (MQ=255)

     gcACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCtt                                                                    <  1:645567/81‑1 (MQ=255)

     gcACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCtt                                                                    >  2:645625/1‑81 (MQ=255)

         cGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATc                                                                <  1:645568/81‑1 (MQ=255)

         cGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATc                                                                >  1:645615/1‑81 (MQ=255)

              aTTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGcc                                                           >  1:645621/1‑81 (MQ=255)

                     ttGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAata                                                      <  2:645569/79‑1 (MQ=255)

                        ttcttcACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTa                                                  >  2:645633/1‑80 (MQ=255)

                        ttcttcACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTAc                                                 <  1:645570/81‑1 (MQ=255)

                             cACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACaa                                               <  1:645571/78‑1 (MQ=255)

                              aCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAg                                            <  1:645572/80‑1 (MQ=255)

                                    cacacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTg                                     >  1:645603/1‑81 (MQ=255)

                                    cacacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTg                                     >  1:645629/1‑81 (MQ=255)

                                      cacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCa                                   <  2:645573/81‑1 (MQ=255)

                                       acaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAGGCCGCCTGCAt                                  <  1:645574/81‑1 (MQ=255)

                                        caTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATa                                 <  1:645575/81‑1 (MQ=255)

                                         aTTCGGTGTGCGGCATGATGTCGGATGCATGGAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAc                                <  2:645576/81‑1 (MQ=255)

                                         aTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAc                                >  2:645619/1‑81 (MQ=255)

                                         aTTCGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAc                                <  1:645577/81‑1 (MQ=255)

                                            cGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATAccc                              <  2:645578/80‑1 (MQ=255)

                                            cGGTGTGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCg                             >  2:645616/1‑81 (MQ=255)

                                              gtgtGCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCgg                            >  1:645617/1‑80 (MQ=255)

                                                gtgCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAg                         >  1:645630/1‑81 (MQ=255)

                                                 tgCGGCATGATGTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGt                        <  2:645579/81‑1 (MQ=255)

                                                          atgTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACtt               <  2:645580/81‑1 (MQ=255)

                                                          atgTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACt                >  2:645623/1‑80 (MQ=255)

                                                          atgTCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCAGCCTGCATACCCGGAAGTACACGACtt               <  2:645581/81‑1 (MQ=255)

                                                             tCGGATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTac             <  2:645582/80‑1 (MQ=255)

                                                                gATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGa         <  1:645583/81‑1 (MQ=255)

                                                                gATGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGa         >  2:645634/1‑81 (MQ=255)

                                                                 aTGCATGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGAc        >  2:645620/1‑81 (MQ=255)

                                                                      tGAAGATACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTg   <  2:645584/81‑1 (MQ=255)

                                                                        aagatACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGt  <  2:645585/80‑1 (MQ=255)

GTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGT  >  NC_000913/3397520‑3397671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A