Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F6 I0 R1
|
27 |
48.2 |
1684932 |
90.0% |
1516438 |
148.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,679,525 |
G→T |
100% |
A1074D (GCT→GAT) |
yphG ← |
DUF4380 domain‑containing TPR repeat protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,679,525 | 0 | G | T | 96.1%
| 73.6
/ ‑4.2
| 34 | A1074D (GCT→GAT) | yphG | DUF4380 domain‑containing TPR repeat protein |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base T (13/16); total (13/21) |
| Fisher's exact test for biased strand distribution p-value = 2.53e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGC > NC_000913_3_hsa_tpiA/2679383‑2679671
|
gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAt < 1:492989/149‑1 (MQ=255)
taACAACGGGTTATTTTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGcc < 1:325473/149‑1 (MQ=255)
accgCTTATTCTAATTATTTTGTCATGAACGGCAGCGTGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCaa < 1:13451/146‑1 (MQ=255)
ttAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGa < 1:111194/148‑1 (MQ=255)
gatgaGCGGCAGCGCGTGCCGCTCAAATAATACAGTTAATCGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATAtt < 1:381345/149‑1 (MQ=255)
atgaGGGGCAGGGCGTGCCGTTCAAATATTACAGTTAATAGGGTTTAGTGAACGAGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTg < 2:12849/149‑1 (MQ=255)
cGCTCAAATATTACAGTTAATAGACTTTAGTAAACTCGGTTAAAAATAAAAGACATGACGGTGGTGAAAGGTGCCTCTTTCGCTCCATCCGGATTAATCGCCGTCAATTCAGGGCGAGCTGACTCATTTTTCGCAACCTCCCCCAGccc > 2:832627/1‑149 (MQ=255)
gCTGAAATGTTATAGTTAATAGGTTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAACTCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc < 2:724323/149‑1 (MQ=255)
gCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTccc > 1:223035/1‑149 (MQ=255)
cTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcg > 2:590131/1‑149 (MQ=255)
aaaaTATTATGTTTTATAAGTTTTATGGGACGCGGTAAAAAATAAAAGTTATCACGGTAGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGCCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcgcg < 2:797402/148‑1 (MQ=255)
aaaTATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATAGCCGTCAATTCAGCGCGAGCAGACCCATATTGCGCAACCCCCCCCAGTCCcgccg > 2:287897/1‑147 (MQ=255)
aGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa > 1:334301/1‑149 (MQ=255)
aGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCCTACGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCCCAGACGccacca > 2:724283/1‑149 (MQ=255)
ttAATAGGGTTTATTGAACGCGGTTAAAAATAAAAGGCATCACGGGGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATc < 2:465960/149‑1 (MQ=255)
tgaagtaaCGGGGTTAATAATAAAAGGCGTCACGGTGGTGAATAATGCCGCTTTCGGACAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCa < 2:2203/143‑1 (MQ=255)
gTGAACGCGGTTTAAAATGAAAGGCATCACGGTGGTGGATAATGCCGCTTTCGGCCAATCCGGGGTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCGGCGGGCGGCAGCATCGCCACCATCAGGc < 2:816240/149‑1 (MQ=255)
cgcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTGATCGCCGTCACTTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAtttt > 2:515318/1‑149 (MQ=255)
ttAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGATTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCttt < 2:756545/149‑1 (MQ=255)
tAAAAACACAAGGCATCAGGGTGGTGANTACTNCGGCTTTCGGCCAACCCGGTTTAATCGCCGTCAATTCAGCGCGATCAGCCTCATATTGCGCAACCCCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTa < 1:185628/149‑1 (MQ=255)
aaaaaTAAAAGGCATCACGGTGGTGAATAATTTCGCTTTCGGAGGCGTTGAAAGAATCTCCGTAAATTCAGCGTTAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAt < 1:611259/149‑1 (MQ=255)
aaaaaTAAAAGGCATCACGGTGGTGAATAATGCCGCGTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAt > 2:387015/1‑149 (MQ=255)
aaaaTAAAAGGGGTCACGGTGGGGAATAATGCCGCTTGCGGCACATCCGGTTTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCACTCTCCCCGAGTCCCTTGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtg < 2:328519/149‑1 (MQ=255)
aaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCCTTTTTCTTTAtgct > 2:651905/1‑149 (MQ=255)
aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGCCTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTaa > 1:278284/1‑149 (MQ=255)
tCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTaaa > 2:418462/1‑149 (MQ=255)
tCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCCGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTaaa > 2:208730/1‑149 (MQ=255)
aCGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCTACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATc < 2:738750/149‑1 (MQ=255)
ataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGACCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCCGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGc < 1:349023/149‑1 (MQ=255)
gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGCCGCCAGCATCGCCACCATCAGGCATTTTGCTTTATGCTGCCGTTGTAAATCGCCAAACAGCGacga > 1:685079/1‑147 (MQ=255)
gCTTTCGGCCAATCCGGATCAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa < 1:359377/149‑1 (MQ=255)
tCGGCCAATTCGTATTAATCGCCGTCAATGCAGGGCGAGCAGAGTCATATTGCGCAACCTCCCCGAGTCCCCTGGTCGCCCGCATCGCCACCATTAGGCATTCTGCTTTATGCTGCTGTTGTATATCGCCATACAGCGACAACAAGTCa < 2:699170/149‑1 (MQ=255)
cGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAg < 1:348333/149‑1 (MQ=255)
aaGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGc < 2:184942/149‑1 (MQ=255)
|
GTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTTGC > NC_000913_3_hsa_tpiA/2679383‑2679671
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
