Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F34 I1 R1
|
26 |
24.1 |
841528 |
92.8% |
780937 |
143.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,679,525 |
G→T |
A1074D (GCT→GAT) |
yphG ← |
DUF4380 domain‑containing TPR repeat protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,679,525 | 0 | G | T | 100.0%
| 59.5
/ NA
| 22 | A1074D (GCT→GAT) | yphG | DUF4380 domain‑containing TPR repeat protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (16/6); total (16/6) |
GGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT > NC_000913_3_hsa_tpiA/2679382‑2679669
|
gttttttAGTATCAGCGTCTTATTGTAATGGTTTGGTGATGGGCGTGGGGTTTTGCCGATAAAATATTATAGTTGATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGa < 2:30812/147‑1 (MQ=255)
ggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGa > 1:417841/1‑149 (MQ=255)
gtatttACTATCAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAACAATGCCGCTTTCGGCCAATCCGGAt > 2:367408/4‑149 (MQ=255)
gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGAt > 1:90878/1‑149 (MQ=255)
aaTTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTAGGGTTAGTAGGGTTTAGTGGACTCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGACCAATCAGGATTAATCGCCGTCAATTCAgagcgagc > 2:195964/1‑149 (MQ=255)
gcggcgcgCGGTAGCGCGTGACGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCata < 1:195964/142‑1 (MQ=255)
atgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCACTTCAGCGCGAGCAGACTCATATTg > 2:369998/1‑149 (MQ=255)
tgaGAGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCCTTCGGCCAATCCGGATTAATCGCCGTCAATTCCGCGCGAGCAGACCCATATTgc > 2:319903/1‑149 (MQ=255)
ccGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTc > 1:313133/1‑149 (MQ=255)
cGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAATcc > 2:353158/1‑149 (MQ=255)
tCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCGCGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGCCCcgc > 2:418747/1‑149 (MQ=255)
aaTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCAGATTTAATCGCCTTCAATTCAGCGCGATCCGACTCATATTGCGCAACCTCCCCGAGTCCCGCCGACGCCAGCATCGc > 2:212354/1‑149 (MQ=255)
tttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGCCCTGCGGACGCCAGCATCGcccccaac > 2:254160/1‑147 (MQ=255)
gTGAACGCGGTTAAAAATAAAAGAAAAGA‑GTTGTGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGc > 2:302699/1‑149 (MQ=255)
atgaaaaaTAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTCCtc < 2:323187/146‑2 (MQ=255)
ttAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGACCAATCCGGATTAATCGCCGTCACTTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCCTCAGGCATTTTTCttt > 2:139008/1‑149 (MQ=255)
aaaTAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGACCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgc > 2:28559/1‑149 (MQ=255)
aaTAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgct > 2:181269/1‑149 (MQ=255)
aaaaGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATGGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATgctgct < 1:418747/149‑1 (MQ=255)
gCCGCTTTCGGCCAGTCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGacaa < 1:346360/149‑1 (MQ=255)
gCTGTCGGGCAATCCGGATTAGTCGCCGGCAATTGGGCGCGTGTACACTTTTTTTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa < 2:299192/149‑1 (MQ=255)
ccAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGCCAACAAGTCAGGtt > 1:411498/1‑149 (MQ=255)
|
GGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTG‑GTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT > NC_000913_3_hsa_tpiA/2679382‑2679669
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
