Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F9 I0 R1
|
23 |
38.9 |
1378630 |
88.6% |
1221466 |
148.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,679,525 |
G→T |
100% |
A1074D (GCT→GAT) |
yphG ← |
DUF4380 domain‑containing TPR repeat protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,679,525 | 0 | G | T | 100.0%
| 92.0
/ ‑6.7
| 35 | A1074D (GCT→GAT) | yphG | DUF4380 domain‑containing TPR repeat protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (15/18); total (16/19) |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT > NC_000913_3_hsa_tpiA/2679385‑2679669
|
ttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCTTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTa > 2:456525/1‑149 (MQ=255)
attaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGCGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAACGCATCACGGTACTGAATAATGCCGCTTCCGGCCAATCGGGATCAATCg > 2:149365/1‑149 (MQ=255)
taACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATCGGGTCTGGTGGCAGCGGGTAAAAATGAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGcc > 2:417015/1‑149 (MQ=255)
taACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGcc < 1:483065/149‑1 (MQ=255)
ggCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAtt < 1:192662/149‑1 (MQ=255)
attattTTGTGGCGAGCGGCAGCGNGTGTCGCTCAAATATTTCAGTTAATAGGGTTTAGTGAACGCGGGTAAAAATAAAAGGAACAACGGGTGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCa < 1:97562/149‑1 (MQ=255)
gatgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCAGCGTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCACGAGCAGACTTATAtt > 1:361055/1‑149 (MQ=255)
gcgGGCCGCTCAATTATTACAGTTAATAGTGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCGCGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTACTCGCCGTAAATTCAGCGCGAGTAGACTCATATTGCGCAAACTcccc < 2:400418/149‑1 (MQ=255)
gTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAGGCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGa < 1:681160/149‑1 (MQ=255)
ctGCCGCTCAAATATCACAGTTAATAGGCTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGa < 1:645852/148‑1 (MQ=255)
tCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCcgc < 1:215779/149‑1 (MQ=255)
atTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATACCGCGCGGCTCGATGGTGACTAATGCTGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCCCAACCTCCCCGGGTCCCGCGGACGc > 1:679018/1‑149 (MQ=255)
cAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTCCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTACGCAACCTCCCCGAGTCCCGCCGACGCCCGc > 2:466651/1‑149 (MQ=255)
aGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCa > 1:185542/1‑149 (MQ=255)
ttAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATc > 1:683695/1‑149 (MQ=255)
aaaGGGTTGAGTGAACGCGGTTAAAAATAAAAGGCAGCACGGTGGTGAATTATGCCGCCTTCGGCCTATCCGGATCAATCGCCGTCAATTCAGCGCGAGCAGACTGCTATGGTGCAACCCTCCCGAGTCCCGGGGACGCCAGCATCGcc < 2:176847/147‑1 (MQ=255)
tAGGGGTTAGGGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCCGCGCGAGCAGACTCATATTACGCAACCTCCCCCACTCCCCCGGCCGCCAGTATCCcca > 2:234448/1‑149 (MQ=255)
ggTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGccacca > 1:484749/1‑149 (MQ=255)
aGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAgg < 2:123446/149‑1 (MQ=255)
gTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCACGAGTCCCGCGGACGCCAGCATCGCCACCATCCGGc > 1:485215/1‑149 (MQ=255)
aaCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAtt < 1:165861/149‑1 (MQ=255)
ctgggTTAAAAATAAAAGGGATCACGTTGGTTAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTTCCGCGTACGCTAGTATCGCCACCATCAGGCAtttt < 2:223571/146‑1 (MQ=255)
gcgGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAttttt < 1:391905/149‑1 (MQ=255)
tAAAAATAGAAGGCTTCACGGTGGTGAATAATGCCGCTTTCGGTCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGGCACCATCAGGCATTTTTCTTTa < 2:120349/149‑1 (MQ=255)
aaaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgc > 2:426532/1‑149 (MQ=255)
aaaGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCCTCAGGCATTTTTCTTTATGctgctg > 1:26073/1‑149 (MQ=255)
aGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCtgtt < 1:4067/149‑1 (MQ=255)
tCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATAGCCGTCAATTCAGCGCGAGCAGACTCAAATTGCGCAACCTCCCCGAGTCCCGCGGAAGCCAGCATCTCCACCATCAGGCATTTTTCTTTCAGCTGCTGTTGTaaa > 1:143035/1‑149 (MQ=255)
aCGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATc < 1:512138/149‑1 (MQ=255)
aCGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCCCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATc > 2:336628/1‑149 (MQ=255)
gtggtgAATAATGCCGCTTTCGGCCAATCCGGATTAATGGCCGTCAATTCAGCGCGAGCAGACTCATATTGAGCAACCTCCGCGGTTCCCGCGGACGCCAGCATCGCCACCATCAAGCCTTTTTCTTTATGCTGCTGTTGTAAATCGcc > 2:585303/1‑149 (MQ=255)
aataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAg < 1:93532/149‑1 (MQ=255)
aatGCCGCTTTCGGCCAATCCGGATTAATCGCCGCCAATTCAACTCTAGCAGACTCATATTGCGCAACCTCCCCGAGGCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGa < 1:17140/149‑1 (MQ=255)
gCCAATCCGGATTAATCGTCGTCAACTCAGCTCTAGCAGACTCATATCCGACACCCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTCTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGt < 1:91128/149‑1 (MQ=255)
cgaaGCCGGGTTAATGGCGGTCAATTCAGCGCGAGTAGACTCATTTTGGGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGtt < 2:110769/147‑1 (MQ=255)
|
TTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGTCAGGTT > NC_000913_3_hsa_tpiA/2679385‑2679669
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
