Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F40 I0 R1
|
23 |
39.3 |
1381890 |
89.2% |
1232645 |
148.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913_3_hsa_tpiA |
2,679,525 |
G→T |
100% |
A1074D (GCT→GAT) |
yphG ← |
DUF4380 domain‑containing TPR repeat protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_hsa_tpiA | 2,679,525 | 0 | G | T | 96.8%
| 79.0
/ ‑5.3
| 29 | A1074D (GCT→GAT) | yphG | DUF4380 domain‑containing TPR repeat protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (13/15); minor base A (0/1); total (13/16) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 |
| Rejected as polymorphism: E-value score below prediction cutoff. |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGT > NC_000913_3_hsa_tpiA/2679382‑2679663
|
ggTTTTTATTAACAACGGCTTATTCGAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAAAAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGa < 1:623557/149‑1 (MQ=255)
attaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCAGCTTTCGGCCAATCTGGATTAATCg > 1:397063/1‑149 (MQ=255)
taACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGcc < 1:381840/149‑1 (MQ=255)
aacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGGGCGTGCCGCTCAAATATTACAGTTAAGAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCa < 1:16547/149‑1 (MQ=255)
tAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTAAAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAg > 1:663616/1‑149 (MQ=255)
tggtgaGCGGCAGCTCGTGCCGCTCAAATATTACAATGAATAGGGTTTAGTGAACGCGGTTGAAAATAAAAGGCATCACGGCGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCTCCGTCAATTCAGCGCGAGCAGACTCatat < 1:330972/146‑1 (MQ=255)
tgatgaGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATTACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCCCGAGCATACCCatat > 2:239797/1‑149 (MQ=255)
tgatgaGCGGCAGCGCGTGCCGCTCAAATATTACAGGTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCCATCCGGATTCATCCCCGTCAATCAAGCCCCAGCAGCCTccatc > 2:311334/1‑145 (MQ=255)
tgggCGGCAGGGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTgc < 2:504762/146‑1 (MQ=255)
gCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGGCTCATATTGCGCAAcc > 1:87330/1‑149 (MQ=255)
cAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCt > 1:523148/1‑149 (MQ=255)
aaTATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGa < 1:346092/149‑1 (MQ=255)
tACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCa > 1:483644/1‑149 (MQ=255)
gTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCTAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCAt < 1:653039/149‑1 (MQ=255)
aGTGAAAGGGGTTAAAAATAAAAGGCGTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAgg < 2:161236/149‑1 (MQ=255)
ggTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGCAAAGTCCGCTCCTTCATCGCCACCATCAGGCATTTTTCt < 1:164169/149‑1 (MQ=255)
ttAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCttt > 1:157048/1‑149 (MQ=255)
aaaaTAAAAGGCATCACGGCGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtg < 1:387007/149‑1 (MQ=255)
aaaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCGTTAtgc > 1:162262/1‑149 (MQ=255)
aGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCtttt > 1:81491/1‑149 (MQ=255)
aGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCAACAGGCATTTTACTTTATGCTGCtgta > 1:225834/1‑148 (MQ=255)
tcgctggtgAATAATGCCTCTTGCGGCCAATCCGGATTAATAGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTaaaac < 2:138050/145‑3 (MQ=255)
ttgttaatattGTCGCTTTCTGCCATTCGTGATTAATCGCCGTTTTTTGTGCGCTAGCAGACTCATATCGCGCAGCTTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCACCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCa < 1:277884/144‑1 (MQ=255)
aataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAg > 2:556071/1‑149 (MQ=255)
ataatGTCGGTTTCGGCCAATCCTGATTAATCGCCGTCAATTCAGCGCGAGCATACTCGTATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGc < 2:617516/149‑1 (MQ=255)
ataatGCCGCTTTCGGCCAATGCGGATTAAGCGCCGTCAATTCAGCGCGAGCAGCCTCATAACGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTCTTCCTCACGCGGTTGTTGTAAATCGCCATACAGc < 2:524294/149‑1 (MQ=255)
aatGGCGCTTTCGGCCAATTCGGATTAATCGCCGTCCATTCAGCGCGTGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGa < 2:443775/149‑1 (MQ=255)
tgcttgggCCCAACCGGATTAATCGCCGGTAATTCCGCGCGAGCAGTCTCATATTGCGCAATCTCCTCGAGTCACGCGGACGCCAGCATCGGCACGATCAGGCATTTTTTTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa < 2:339660/143‑1 (MQ=255)
tttCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCCGCATCGCCACCATCAGGCCTTTTTCTTTATGATGCTGTTGTAAATCCCCATACAGGGAcaacaact > 2:129209/1‑147 (MQ=255)
|
GGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAAGT > NC_000913_3_hsa_tpiA/2679382‑2679663
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
