Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A16 F19 I2 R1	17	101.9	4194818	93.7%	3930544	121.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	3,440,435	C→A	D199Y (GAC→TAC)	rpoA ←	RNA polymerase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	3,440,435	0	C	A	98.7%	265.4 / NA	77	D199Y (GAC→TAC)	rpoA	RNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (40/36); minor base T (1/0); total (41/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACATAACC  >  NC_000913_3_pae_tpiA/3440288‑3440579

ctGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACa                                                                                                                                                <  2:1042368/150‑1 (MQ=255)

ctGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACa                                                                                                                                                <  3:620554/150‑1 (MQ=255)

 tGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAg                                                                                                                                               <  1:1067019/150‑1 (MQ=255)

 tGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAg                                                                                                                                               >  4:120840/1‑150 (MQ=255)

    tttCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTc                                                                                                                                            >  2:1136164/1‑150 (MQ=255)

         cttcttTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGCTTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTAc                                                                                                                                       >  3:17043/1‑150 (MQ=255)

          ttcttTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACg                                                                                                                                      >  2:93298/1‑150 (MQ=255)

             tttCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTg                                                                                                                                   >  4:786720/1‑150 (MQ=255)

              ttCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGt                                                                                                                                  >  3:171762/1‑150 (MQ=255)

                  cTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTa                                                                                                                              <  4:514671/150‑1 (MQ=255)

                       ggCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACAcgc                                                                                                                         <  1:1136164/150‑1 (MQ=255)

                          tGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCgct                                                                                                                      >  4:123435/1‑150 (MQ=255)

                              gTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCtt                                                                                                                  <  2:1188161/150‑1 (MQ=255)

                                  aTCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAAc                                                                                                              >  3:24602/1‑150 (MQ=255)

                                  aTCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAAc                                                                                                              <  3:786720/150‑1 (MQ=255)

                                   tCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACa                                                                                                             >  2:748670/1‑150 (MQ=255)

                                      cGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCTTATCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACAttt                                                                                                          >  1:671653/1‑149 (MQ=255)

                                      cGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTg                                                                                                          <  1:93298/150‑1 (MQ=255)

                                        tAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTa                                                                                                        <  3:120840/150‑1 (MQ=255)

                                            tCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACg                                                                                                                                      >  2:61605/1‑116 (MQ=255)

                                            tCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACg                                                                                                                                      <  1:61605/116‑1 (MQ=255)

                                                 gAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCt                                                                                                                                    <  2:29704/113‑1 (MQ=255)

                                                 gAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCt                                                                                                                                    >  1:29704/1‑113 (MQ=255)

                                                 gAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACg                                                                                               >  2:583311/1‑150 (MQ=255)

                                                         ccAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTac                                                                                                                             <  2:91662/112‑1 (MQ=255)

                                                         ccAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTac                                                                                                                             >  1:91662/1‑112 (MQ=255)

                                                            gttgttCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGt                                                                                                                                             <  3:493020/93‑1 (MQ=255)

                                                            gttgttCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGt                                                                                                                                             >  4:493020/1‑93 (MQ=255)

                                                              tgttCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTg                                                                                  <  1:583311/150‑1 (MQ=255)

                                                               gttCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGt                                                                                 <  4:171762/150‑1 (MQ=255)

                                                                    gCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACAcgc                                                                                                                         >  3:216657/1‑105 (MQ=255)

                                                                    gCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACAcgc                                                                                                                         <  4:216657/105‑1 (MQ=255)

                                                                     ccAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATg                                                                           >  4:219917/1‑150 (MQ=255)

                                                                        gAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGt                                                                        <  1:748670/150‑1 (MQ=255)

                                                                                    cACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCgct                                                                                                                      <  2:598616/92‑1 (MQ=255)

                                                                                    cACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCgct                                                                                                                      >  1:598616/1‑92 (MQ=255)

                                                                                     acgacgAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACg                                                           >  1:1246810/1‑150 (MQ=255)

                                                                                            aTCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGAtt                                                    <  3:123435/150‑1 (MQ=255)

                                                                                                ccTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGAt                                                     <  2:718582/145‑1 (MQ=255)

                                                                                                ccTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGAt                                                     >  1:718582/1‑145 (MQ=255)

                                                                                                ccTCTTCAGGATCGATTGTGCCGTTGGTTACCATTTAAATGATCATCGTCTTTAAGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTACACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGgc                                                >  4:35819/1‑150 (MQ=255)

                                                                                                  tctTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGAc                                                                    <  4:399547/128‑1 (MQ=255)

                                                                                                  tctTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGAc                                                                    >  3:399547/1‑128 (MQ=255)

                                                                                                  tctTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGgcgc                                              >  3:192651/1‑150 (MQ=255)

                                                                                                    ttCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCaa                                                                                                   >  1:653106/1‑95 (MQ=255)

                                                                                                    ttCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCaa                                                                                                   <  2:653106/95‑1 (MQ=255)

                                                                                                    ttCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTc                                            >  1:1149875/1‑150 (MQ=255)

                                                                                                     tCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCa                                           >  3:409318/1‑150 (MQ=255)

                                                                                                         gATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGAtt                                                    >  4:261162/1‑137 (MQ=255)

                                                                                                         gATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGAtt                                                    <  3:261162/137‑1 (MQ=255)

                                                                                                          aTCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCAtcttc                                      >  4:36013/1‑150 (MQ=255)

                                                                                                          aTCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCAtcttc                                      <  4:650362/150‑1 (MQ=255)

                                                                                                           tCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACcagca                                                               <  4:174203/124‑1 (MQ=255)

                                                                                                           tCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACcagca                                                               >  3:174203/1‑124 (MQ=255)

                                                                                                              aTTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTcc                                  <  1:319328/150‑1 (MQ=255)

                                                                                                                tgtgCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCAtcttc                                      >  2:287848/1‑144 (MQ=255)

                                                                                                                tgtgCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCAtcttc                                      <  1:287848/144‑1 (MQ=255)

                                                                                                                  tgCCGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCgaat                              <  3:219917/150‑1 (MQ=255)

                                                                                                                    ccGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCgaatga                            >  1:748542/1‑150 (MQ=255)

                                                                                                                     cGTTGGTTTCCATTTCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGGGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCgaatgaa                           >  1:767658/1‑150 (MQ=255)

                                                                                                                                 tttCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAAc                                                                                                              >  2:966949/1‑55 (MQ=255)

                                                                                                                                 tttCGATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAAc                                                                                                              <  1:966949/55‑1 (MQ=255)

                                                                                                                                     gATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCgg           >  3:268020/1‑150 (MQ=255)

                                                                                                                                     gATGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCgg           >  2:1104047/1‑150 (MQ=255)

                                                                                                                                      aTGACCAGCTTGTACAGGTTGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGc                                                                          >  2:109297/1‑86 (MQ=255)

                                                                                                                                      aTGACCAGCTTGTACAGGTTGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGc                                                                          <  1:109297/86‑1 (MQ=255)

                                                                                                                                       tGACCAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGca         <  4:268020/150‑1 (MQ=255)

                                                                                                                                           cAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCaa                                                                                                               >  4:113690/1‑44 (MQ=255)

                                                                                                                                           cAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCaa                                                                                                               <  3:113690/44‑1 (MQ=255)

                                                                                                                                           cAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCAtct                                        <  4:615882/115‑1 (MQ=255)

                                                                                                                                           cAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCAtct                                        >  3:615882/1‑115 (MQ=255)

                                                                                                                                           cAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACATa     >  2:438963/1‑150 (MQ=255)

                                                                                                                                           cAGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACATa     >  3:226333/1‑150 (MQ=255)

                                                                                                                                            aGCTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACATaa    <  2:321180/150‑1 (MQ=255)

                                                                                                                                              cTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCg            >  1:414520/1‑140 (MQ=255)

                                                                                                                                              cTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCg            <  2:414520/140‑1 (MQ=255)

                                                                                                                                              cTTGTACAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACATAAcc  <  2:748542/150‑1 (MQ=255)

CTGGTTTCTCTTCTTTCACTTCAGGCTGACGTACATCACGTAAGTCAACGAAAGCTTCCAGTTGTTCAGCCAGAATGGTTGCCGCACGACGAATCGCCTCTTCAGGATCGATTGTGCCGTTGGTTTCCATTTCGATGACCAGCTTGTCCAGGTCGGTACGCTGTTCTACACGCGCTGCTTCAACATTGTAGGCAATACGCTCCACAGGGCTGTAGCATGCGTCGACCAGCAGACGGCCGATTGGGCGCTCATCTTCTTCCGAATGAATTCGGGTAGAAGCCGGCACATAACC  >  NC_000913_3_pae_tpiA/3440288‑3440579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A