Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A24 F106 I2 R1
|
20 |
33.2 |
1150230 |
93.5% |
1075465 |
143.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913_3_pae_tpiA |
2,679,525 |
G→T |
A1074D (GCT→GAT) |
yphG ← |
DUF4380 domain‑containing TPR repeat protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913_3_pae_tpiA | 2,679,525 | 0 | G | T | 100.0%
| 79.8
/ NA
| 26 | A1074D (GCT→GAT) | yphG | DUF4380 domain‑containing TPR repeat protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (10/16); total (10/16) |
TTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAA > NC_000913_3_pae_tpiA/2679384‑2679661
|
tttttATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAtt > 1:34547/1‑149 (MQ=255)
attaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCg > 2:496191/1‑149 (MQ=255)
ttaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGc > 1:65761/1‑149 (MQ=255)
aacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCa > 1:131155/1‑149 (MQ=255)
acGGCTTATTTTAATTGTTTTGTGTGGAGCGGCAGCGCGTGCCGCTGAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGGAGCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATAGCCGTCaa < 2:113568/149‑1 (MQ=255)
cTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCa < 1:29125/149‑1 (MQ=255)
aGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTgcgc > 1:1082/1‑149 (MQ=255)
ggCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAAc < 2:419461/149‑1 (MQ=255)
cgTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATCTCAG‑GCTAGCAGACTCATATTGCGCAACCTCCCCg < 1:569290/149‑1 (MQ=255)
tttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCACT‑TCCGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGCAGCCAGCATCGCCACCATc > 2:349816/1‑149 (MQ=255)
tgtAGTTAACGGTGGTAAAAATAAAAGGCATCATGGTGGTGAATAATGTCGCTTTCGGCCAATCCGGATTAATCGCCGTTAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATc < 2:282323/147‑1 (MQ=255)
ttAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCTCGGACGCCAGCATCGCCACCATCa < 1:496191/149‑1 (MQ=255)
gAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCGACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAt < 1:454074/149‑1 (MQ=255)
aaaaaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAt < 1:536000/149‑1 (MQ=255)
aaaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgc > 1:424868/1‑149 (MQ=255)
aaaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtgc > 1:316055/1‑149 (MQ=255)
ggtggtGAATAAGTCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCACCCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCACCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGc < 1:414755/149‑1 (MQ=255)
gtgAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATa > 1:207209/1‑149 (MQ=255)
aataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAg < 2:316055/149‑1 (MQ=255)
aataatGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAg < 2:424868/149‑1 (MQ=255)
aaggcagCTTTCTGGCAATCCGGATTAATCGCCGCCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGACAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGTGa < 2:183043/143‑1 (MQ=255)
atGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGac > 2:87217/1‑149 (MQ=255)
atGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGac < 1:196071/149‑1 (MQ=255)
gCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGacaa < 2:574561/149‑1 (MQ=255)
gCTTTCGGCCAATCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa < 1:461972/149‑1 (MQ=255)
gCTTTACGCCAATCCGGATTAATCGGCTTCAAT‑TCAGCGCGAGTAGACTCATATTGCGCAAACTCCCCGTGTTTCGATGAGTTGAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa < 2:208140/149‑1 (MQ=255)
|
TTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAAT‑TCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAA > NC_000913_3_pae_tpiA/2679384‑2679661
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
