Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F21 I1 R1	17	36.5	1286766	92.7%	1192832	143.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_tpiA	2,679,525	G→T	A1074D (GCT→GAT)	yphG ←	DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_tpiA	2,679,525	0	G	T	100.0%	87.2 / NA	30	A1074D (GCT→GAT)	yphG	DUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (15/15); total (15/15)

GGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAA  >  NC_000913_3_pae_tpiA/2679381‑2679661

tgggttttATTATCAACGGCTTATTCTAATTATTTTGTGATGGGGGGCAGGGTGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                                                                                                      <  2:518199/145‑1 (MQ=255)

gggTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCgg                                                                                                                                      <  1:398/149‑1 (MQ=255)

  gTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGAt                                                                                                                                    >  1:455347/1‑149 (MQ=255)

      ttattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAAt                                                                                                                                <  1:18812/149‑1 (MQ=255)

       tattaACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATc                                                                                                                               <  1:218815/149‑1 (MQ=255)

           aacaacGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCg                                                                                                                           >  1:502581/1‑149 (MQ=255)

                   cTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAAGAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCa                                                                                                                   <  1:72493/149‑1 (MQ=255)

                         cTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCCATTGGTGTCAATCCGGATTAATCGCCGTCAATTCAGCGCGa                                                                                                             <  1:316522/149‑1 (MQ=255)

                              tattTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGa                                                                                                        <  1:106591/149‑1 (MQ=255)

                                      gatgaGCGGCAGCGCGTGCCACTCAAATATTACAGTTAACAGCGCGTCGCGACCGCGATTAACAATCCAAGGCATTTCGACGTTATATAATGCCGCTTTCGGCCAATCCGGATTAATCCCCCTCAATTCACCGCGCGCAGACTCATCtt                                                                                                >  2:162462/1‑149 (MQ=255)

                                                   gcgTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcccc                                                                                   <  1:186016/149‑1 (MQ=255)

                                                        ccGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTAAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTc                                                                              >  2:359106/1‑149 (MQ=255)

                                                          gCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCATGTCATCGCCAGCAGACTCATATTGCGCAACCTCCCCGAGTccc                                                                            >  1:177554/1‑149 (MQ=255)

                                                            tCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCCAGTccccc                                                                          >  1:585105/1‑147 (MQ=255)

                                                                   ttACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGcc                                                                   >  1:114585/1‑149 (MQ=255)

                                                                    tACAGTTCATATTGTTTAGTGATAGAGGTTAAAAATCAAAGGCGATACGGTAGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCa                                                                  <  2:297477/149‑1 (MQ=255)

                                                                            atatGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcccc                                                                                   <  2:613626/124‑1 (MQ=255)

                                                                            aTAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTcccc                                                                                   >  1:613626/1‑124 (MQ=255)

                                                                             tAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATGAGGGTGGTGAAGAATGCCGCTTTCGGCCAATCCGGCTTAATCGCCGTCAATTCAGGGCGCGCAGCCTGATATTGCGCAACCTCGCGAAGTCCCGCGGGCGCCTGCATCGcca                                                         >  2:339615/1‑149 (MQ=255)

                                                                                   ttAGTGACCGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCa                                                   <  1:74100/149‑1 (MQ=255)

                                                                                       tGAACGCGGTTAAAAATAGAAGGAATCACGGTGGTGAATAATGCCGCTTTCGGCCGATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCAGATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGct                                               >  1:593540/1‑148 (MQ=255)

                                                                                         aaCGCGGTTAAAAATAAAAGTCATCACGGTGGTGAATAATGCTGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCATCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCAtt                                             <  2:472190/149‑1 (MQ=255)

                                                                                                ttAAAAATAAAAGGCATCACGGTGGTGAATAATGCAGCTTTCGGCCAGTCCGGATTAATCGACGCCGATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCttt                                      >  1:604598/1‑149 (MQ=255)

                                                                                                   aaaaTACAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCACTCCGGATTAATCGCCGTCACGTCAGCGCAAGCCGGCTCGGAGGTGGTGACCTTGTCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtg                                   >  1:128228/1‑149 (MQ=255)

                                                                                                   aaaaTAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTAtg                                   <  2:114585/149‑1 (MQ=255)

                                                                                                       tAAAAGGCATCACGGTGGTGAATCTTGAAGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGCGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCCCCATCAGGCATTTTTCTTTAtcctgc                               >  1:479082/1‑149 (MQ=255)

                                                                                                               aTCACGGTGGTGAATAATGCCGCTTTCGGCCAATCCAGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCGTTATGCTGCTGTTGTaa                       >  1:337040/1‑149 (MQ=255)

                                                                                                                                tGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATa              <  1:367430/141‑1 (MQ=255)

                                                                                                                                tGCCGCTTTCGGCCAATCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATa              >  2:367430/1‑141 (MQ=255)

                                                                                                                                    gCTTTCGGCCAATCCTGATTAATCGCCGCCAATTCAGCGCGAGCAGACTCATCTTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGAcaacaa  <  2:632623/149‑1 (MQ=255)

GGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCGCTTTCGGCCAAGCCGGATTAATCGCCGTCAATTCAGCGCGAGCAGACTCATATTGCGCAACCTCCCCGAGTCCCGCGGACGCCAGCATCGCCACCATCAGGCATTTTTCTTTATGCTGCTGTTGTAAATCGCCATACAGCGACAACAA  >  NC_000913_3_pae_tpiA/2679381‑2679661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A