Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	169	80.7	2736954	99.0%	2709584	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,608,220	A→G	L12L (TTA→TTG)	rimI →	ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,608,220	0	A	G	100.0%	155.4 / NA	43	L12L (TTA→TTG)	rimI	ribosomal‑protein‑S18‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/21); total (22/21)

AACCGCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACG  >  NC_000913/4608083‑4608355

aaCCGCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGcc                                                                                                                                       >  1:1303791/1‑140 (MQ=255)

 aCCGCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcg                                                                                                                                      >  1:229737/1‑140 (MQ=255)

    gCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcg                                                                                                                                   <  1:1008557/140‑1 (MQ=255)

    gCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcg                                                                                                                                   <  2:1087025/140‑1 (MQ=255)

    gCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcg                                                                                                                                   <  1:545789/140‑1 (MQ=255)

     cTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcgg                                                                                                                                  >  1:1288712/1‑140 (MQ=255)

     cTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCcggcgg                                                                                                                                  >  1:292317/1‑140 (MQ=255)

         cACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTa                                                                                                                              <  2:229737/140‑1 (MQ=255)

         cACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTa                                                                                                                              <  2:1288712/140‑1 (MQ=255)

         cACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTa                                                                                                                              <  1:674123/140‑1 (MQ=255)

               aaGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAGCACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACAt                                                                                                                        >  1:1185501/1‑140 (MQ=255)

                   cgcTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGaa                                                                                                                    >  2:577557/1‑140 (MQ=255)

                    gcTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGaac                                                                                                                   >  1:894813/1‑140 (MQ=255)

                       cAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGaacaac                                                                                                                <  2:1076213/140‑1 (MQ=255)

                             gCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGccc                                                                                                          >  2:436050/1‑140 (MQ=255)

                              cATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCa                                                                                                         <  2:292317/140‑1 (MQ=255)

                              cATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCa                                                                                                         <  2:1226666/140‑1 (MQ=255)

                                tCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACg                                                                                                       <  2:894813/140‑1 (MQ=255)

                                  aCCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGcc                                                                                                     >  2:760710/1‑140 (MQ=255)

                                          tCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTg                                                                                             >  1:1359670/1‑140 (MQ=255)

                                           cACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTgg                                                                                            >  1:97187/1‑140 (MQ=255)

                                            aCCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTcc                                                                                                <  1:1048975/135‑1 (MQ=255)

                                            aCCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTcc                                                                                                >  2:1048975/1‑135 (MQ=255)

                                                        gCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGt                                                                               >  2:1256120/1‑140 (MQ=255)

                                                          aaaCCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCTCATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGttt                                                                             >  1:594225/1‑140 (MQ=255)

                                                             cccAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGcc                                                                          <  2:1274289/140‑1 (MQ=255)

                                                             cccAACGGCACGCGCCGCGTTATGGCAACAAACTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACACGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGcc                                                                          <  2:1205970/140‑1 (MQ=255)

                                                                   ggCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAGCACGATTTCTTCCCTCGAAACGACTGCTTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAAc                                                                    <  2:1185501/140‑1 (MQ=255)

                                                                       cgcgCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAgg                                                                >  2:354676/1‑140 (MQ=255)

                                                                              cgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCCTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCg                                                         <  2:948010/140‑1 (MQ=255)

                                                                                    ggtaacaaATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTAtct                                                   <  1:577557/137‑1 (MQ=255)

                                                                                     gCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTAtctc                                                  <  2:1359670/140‑1 (MQ=255)

                                                                                        acaaATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAAc                                               >  1:1070291/1‑140 (MQ=255)

                                                                                        acaaATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAAc                                               <  1:436050/140‑1 (MQ=255)

                                                                                                      atGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAAGCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCa                                 >  2:939044/1‑140 (MQ=255)

                                                                                                              gATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCa                         >  1:1042010/1‑140 (MQ=255)

                                                                                                              gATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCa                         >  1:490641/1‑140 (MQ=255)

                                                                                                                tttCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCaaa                       >  1:829861/1‑140 (MQ=255)

                                                                                                                  tcttcCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAAt                     <  2:1303791/140‑1 (MQ=255)

                                                                                                                      cccTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATggcg                 >  2:516515/1‑140 (MQ=255)

                                                                                                                      cccTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATggcg                 <  2:97187/140‑1 (MQ=255)

                                                                                                                                  cTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGAGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGAtt     >  1:1111188/1‑140 (MQ=255)

                                                                                                                                     aTTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACg  <  2:1215477/140‑1 (MQ=255)

AACCGCTATCACTGGAAGGCGCTCAGGTGGCATCACCGGCGCTCACCGATTTACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACG  >  NC_000913/4608083‑4608355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A