breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 54,641 (G)6→7 coding (62/1287 nt) surA ← peptidyl‑prolyl cis‑trans isomerase SurA
RA 69,882 T→C D56G (GAC→GGC)  araB ← ribulokinase
RA 78,265 T→C S215S (AGT→AGC setA → sugar exporter SetA
RA 128,349 T→C G146G (GGT→GGC lpd → lipoamide dehydrogenase
RA 142,194 G→A G159G (GGC→GGT can ← carbonic anhydrase 2
RA 179,819 A→G T195A (ACA→GCA)  dgt → dGTP triphosphohydrolase
RA 232,127 C→T R68C (CGT→TGT)  yafE → putative S‑adenosylmethionine‑dependent methyltransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 275,984 C→T T220T (ACC→ACT mmuP → S‑methyl‑L‑methionine transporter
RA 336,204 T→C S94P (TCC→CCC)  yahE → DUF2877 domain‑containing protein YahE
RA 372,284 T→C V57A (GTA→GCA)  mhpD → 2‑hydroxypentadienoate hydratase
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 412,047 C→T P145P (CCG→CCA araJ ← putative transport protein AraJ
RA 422,637 A→G E40E (GAA→GAG malZ → maltodextrin glucosidase
RA 442,284 C→T P246S (CCG→TCG)  thiI → tRNA uridine 4‑sulfurtransferase
RA 445,956 T→C G237G (GGA→GGG yajR ← putative major facilitator superfamily transport protein YajR
RA 489,266 A→G K911E (AAA→GAA)  mscK → potassium dependent, small conductance mechanosensitive channel
RA 498,410 T→C V119A (GTA→GCA)  hemH → ferrochelatase
RA 508,607 G→A intergenic (+48/+268) ybaQ → / ← copA putative DNA‑binding transcriptional regulator YbaQ/Cu(+) exporting P‑type ATPase
RA 511,219 T→C E54G (GAA→GGA) 
E54G (GAA→GGA) 
copA ←
copA ←
Cu(+) exporting P‑type ATPase
Cu(+) exporting P‑type ATPase
RA 523,741 (G)6→5 coding (481/4281 nt) rhsD → protein RhsD
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 531,186 A→G L14P (CTG→CCG)  selU ← tRNA 2‑selenouridine synthase
RA 533,777 A→G D256G (GAC→GGC)  allR → DNA‑binding transcriptional repressor AllR
RA 594,037 A→G V245A (GTA→GCA)  cusS ← sensory histidine kinase CusS
RA 649,628 C→T R288H (CGT→CAT)  citE ← citrate lyase beta subunit
RA 662,470 A→G V58A (GTA→GCA)  ybeD ← DUF493 domain‑containing protein YbeD
RA 730,736 A→G D385G (GAC→GGC)  rhsC → rhs element protein RhsC
RA 796,655 T→C G34G (GGT→GGC modC → molybdate ABC transporter ATP binding subunit
RA 807,017 T→C T89A (ACT→GCT)  ybhC ← outer membrane lipoprotein YhbC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 892,418 T→C V151A (GTC→GCC)  rimK → ribosomal protein S6 modification protein
RA 923,993 A→G T244A (ACG→GCG)  clpA → ATP‑dependent Clp protease ATP‑binding subunit ClpA
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,014,202 T→C V315A (GTA→GCA)  pqiB → intermembrane transport protein PqiB
RA 1,029,596 A→G V125A (GTG→GCG)  rlmI ← 23S rRNA m(5)C1962 methyltransferase
RA 1,039,673 C→T G126G (GGC→GGT appB → cytochrome bd‑II ubiquinol oxidase subunit II
RA 1,100,871 A→G S272P (TCG→CCG)  csgG ← curli secretion channel
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,212,816 C→T A98T (GCG→ACG)  ycgX ← uncharacterized protein YcgX
RA 1,213,895 A→G R55R (CGT→CGC bluR ← DNA‑binding transcriptional repressor BluR
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,276,807 A→G V271A (GTA→GCA)  narX ← sensory histidine kinase NarX
RA 1,280,410 C→T P183S (CCG→TCG)  narG → nitrate reductase A subunit alpha
RA 1,310,788 T→C A26A (GCA→GCG yciI ← protein YciI
RA 1,322,634 Δ1 bp coding (313/1563 nt) trpE ← anthranilate synthase subunit TrpE
RA 1,335,943 T→C L38P (CTA→CCA)  acnA → aconitate hydratase 1
RA 1,399,985 (C)7→8 coding (252/516 nt) ogt ← methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,434,769 T→G intergenic (‑363/+189) ynaM ← / ← ttcC Rac prophage; protein YnaM/putative tRNA 2‑thiocytidine (s(2)C32) synthase
RA 1,529,396 T→C pseudogene (1507/2049 nt) rhsE → RhsE protein in rhs element
RA 1,529,541 T→C pseudogene (1652/2049 nt) rhsE → RhsE protein in rhs element
RA 1,537,145 C→T W55* (TGG→TGA narW ← NarW, putative private chaperone for NarZ nitrate reductase subunit
RA 1,537,724 A→G L377P (CTG→CCG)  narY ← nitrate reductase Z subunit beta
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,590,218 T→C intergenic (‑217/+116) ydeT ← / ← yneL fimbrial usher domain‑containing protein YdeT/putative HTH‑type transcriptional regulator YneL
JC 1,594,132 (C)8→11 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,635,384 T→C E155G (GAA→GGA)  ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,817,644 A→G V286A (GTA→GCA)  chbF ← monoacetylchitobiose‑6‑phosphate hydrolase
RA 1,847,928 T→C E27E (GAA→GAG selD ← selenide, water dikinase
RA 1,872,148 T→C S36S (AGT→AGC dgcJ → putative diguanylate cyclase DgcJ
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,933,347 T→C T420A (ACA→GCA)  edd ← phosphogluconate dehydratase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,012,563 (G)6→7 pseudogene (62/279 nt) intG → putative defective phage integrase
RA 2,064,847 G→A L126L (CTG→TTG)  cobS ← cobalamin 5'‑phosphate synthase
RA 2,084,911 T→C V205V (GTA→GTG yeeE ← inner membrane protein YeeE
RA 2,102,890 T→C R8G (AGA→GGA)  insH‑7 ← IS5 transposase and trans‑activator
RA 2,155,225 A→G T404A (ACC→GCC)  mdtA → multidrug efflux pump membrane fusion protein MdtA
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,210,467 T→C R456R (CGT→CGC yehQ → SWIM zinc finger domains‑containing protein YehQ
RA 2,288,561 (C)7→6 pseudogene (354/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,303,697 Δ1 bp coding (92/165 nt) yojO → uncharacterized protein YojO
RA 2,351,405 T→C T324A (ACC→GCC)  glpT ← sn‑glycerol 3‑phosphate:phosphate antiporter
RA 2,380,598 A→G S16P (TCG→CCG)  menF ← isochorismate synthase MenF
RA 2,443,211 T→C V483A (GTC→GCC)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,467,734 T→C intergenic (+32/‑121) intS → / → yfdG CPS‑53 (KpLE1) prophage; prophage CPS‑53 integrase/CPS‑53 (KpLE1) prophage; putative bactoprenol‑linked glucose translocase
RA 2,521,497 G→A intergenic (+169/+96) lysV → / ← xapR tRNA‑Lys/DNA‑binding transcriptional activator XapR
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,566,179 T→C T236A (ACG→GCG)  eutA ← reactivating factor for ethanolamine ammonia‑lyase EutBC
RA 2,567,131 T→C N326S (AAC→AGC)  eutH ← putative inner membrane protein
RA 2,602,017 (C)7→6 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,618,225 T→C E184E (GAA→GAG hda ← inibitor of reinitiation of DNA replication
RA 2,658,666 T→C G90G (GGA→GGG hscA ← iron‑sulfur cluster biosynthesis chaperone HscA
RA 2,717,821 (C)5→6 coding (708/1038 nt) yfiF ← putative methyltransferase
RA 2,734,543 (C)8→7 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,757,775 (T)6→7 coding (826/957 nt) yfjH ← CP4‑57 prophage; protein YfjH
RA 2,775,830 A→G intergenic (+14/‑89) ypjI → / → yfjX putative UPF0401 protein YpjI/CP4‑57 prophage; putative antirestriction protein
RA 2,790,113 C→G G44G (GGC→GGG lhgO → L‑2‑hydroxyglutarate oxidase
RA 2,806,078 C→T A23A (GCC→GCT proW → glycine betaine ABC transporter membrane subunit ProW
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,829,052 A→G K2K (AAA→AAG srlR → DNA‑binding transcriptional repressor SrlR
RA 2,831,394 A→G L299P (CTG→CCG)  norR ← DNA‑binding transcriptional dual regulator NorR
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,875,172 T→C D83G (GAC→GGC)  cysN ← sulfate adenylyltransferase subunit 1
RA 2,917,664 A→G S870G (AGC→GGC)  barA → sensory histidine kinase BarA
RA 2,925,575 T→C G76G (GGT→GGC queF → 7‑cyano‑7‑deazaguanine reductase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,961,100 A→G R443R (CGT→CGC recC ← exodeoxyribonuclease V subunit RecC
RA 2,973,283 G→A Q194* (CAG→TAG)  lplT ← lysophospholipid transporter
RA 2,981,542 G→A A214V (GCG→GTG)  araE ← arabinose:H(+) symporter
RA 3,001,819 T→C V492A (GTT→GCT)  xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,070,708 G→A T179T (ACC→ACT fbaA ← fructose‑bisphosphate aldolase class II
RA 3,152,571 A→G E112E (GAA→GAG metC → cystathionine beta‑lyase/L‑cysteine desulfhydrase
RA 3,164,769 G→A A402V (GCG→GTG)  parC ← DNA topoisomerase IV subunit A
RA 3,179,863 T→C S40S (AGT→AGC ygiB → DUF1190 domain‑containing protein YgiB
RA 3,180,607 G→C V63L (GTG→CTG)  ygiC → putative enzyme with ATPase activity
RA 3,213,348 A→G Y101C (TAC→TGC)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,234,758 T→C K373R (AAG→AGG)  rlmG ← 23S rRNA m(2)G1835 methyltransferase
RA 3,253,814 A→G L134P (CTG→CCG)  yhaJ ← DNA‑binding transcriptional activator YhaJ
RA 3,324,469 A→G M148T (ATG→ACG)  folP ← dihydropteroate synthase
RA 3,340,902 A→G T210A (ACG→GCG)  yrbG → putative transport protein YrbG
RA 3,340,984 A→C N237T (AAC→ACC)  yrbG → putative transport protein YrbG
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,415,885 C→T P285S (CCG→TCG)  acrF → multidrug efflux pump RND permease AcrF
RA 3,422,035 C→T G237G (GGC→GGT yhdY → putative ABC transporter membrane subunit YhdY
RA 3,432,994 (C)6→5 coding (567/1125 nt) smf ← protein Smf
RA 3,543,262 A→G L32L (TTA→TTG rpnA → recombination‑promoting nuclease RpnA
RA 3,547,951 A→G intergenic (+76/+35) gntT → / ← malQ high‑affinity gluconate transporter/4‑alpha‑glucanotransferase
RA 3,549,792 (C)6→7 coding (279/2085 nt) malQ ← 4‑alpha‑glucanotransferase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,633,068 T→C pseudogene (1391/1607 nt) yhiL ← putative uncharacterized protein YhiL
RA 3,663,316 Δ1 bp coding (2903/3114 nt) mdtF → multidrug efflux pump RND permease MdtF
RA 3,667,956 T→C A411A (GCA→GCG ccp ← cytochrome c peroxidase
RA 3,805,061 T→C Y19C (TAC→TGC)  waaS ← lipopolysaccharide core biosynthesis protein
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,833,576 T→C D321G (GAC→GGC)  yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,839,566 A→G L143P (CTT→CCT)  nlpA ← lipoprotein‑28
RA 3,846,450 T→C T240A (ACA→GCA)  uhpT ← hexose‑6‑phosphate:phosphate antiporter
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,878,207 T→C N638S (AAC→AGC)  gyrB ← DNA gyrase subunit B
RA 3,884,387 T→C F18L (TTC→CTC)  rpmH → 50S ribosomal subunit protein L34
RA 3,890,927 T→C V231A (GTA→GCA)  tnaB → tryptophan:H(+) symporter TnaB
RA 3,935,222 C→T A482V (GCC→GTC)  rbsA → ribose ABC transporter ATP binding subunit
RA 3,939,658 T→C T319A (ACC→GCC)  hsrA ← putative transporter HsrA
RA 3,939,781 T→C T278A (ACC→GCC)  hsrA ← putative transporter HsrA
RA 3,980,957 (G)6→7 coding (71/1386 nt) yifK → putative transporter YifK
RA 4,009,923 T→C L252L (TTA→CTA)  pldB → lysophospholipase L2
RA 4,060,280 (T)9→8 intergenic (+50/‑167) bipA → / → yihL ribosome‑dependent GTPase, ribosome assembly factor/putative transcriptional regulator YihL
RA 4,141,044 G→A A393V (GCG→GTG)  ptsA ← putative PTS multiphosphoryl transfer protein PtsA
RA 4,152,473 A→G V209A (GTG→GCG)  ppc ← phosphoenolpyruvate carboxylase
RA 4,157,589 T→C V247A (GTG→GCG)  argH → argininosuccinate lyase
RA 4,243,814 A→G L421P (CTG→CCG)  malF ← maltose ABC transporter membrane subunit MalF
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,329,894 (G)6→7 coding (535/879 nt) yjcZ → uncharacterized protein YjcZ
RA 4,379,308 G→A A20V (GCC→GTC)  frdD ← fumarate reductase membrane protein FrdD
RA 4,380,030 C→T G163D (GGC→GAC)  frdB ← fumarate reductase iron‑sulfur protein
RA 4,390,183 A→G Y60H (TAC→CAC)  psd ← phosphatidylserine decarboxylase proenzyme
RA 4,443,679 A→G N433S (AAC→AGC)  tamA → translocation and assembly module subunit TamA
RA 4,445,095 C→T G328G (GGC→GGT tamB → translocation and assembly module subunit TamB
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,550,283 C→T S338S (TCG→TCA gntP ← fructuronate transporter
RA 4,558,802 A→G L242P (CTG→CCG)  iadA ← isoaspartyl dipeptidase
RA 4,559,111 A→G V139A (GTC→GCC)  iadA ← isoaspartyl dipeptidase
RA 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,608,220 A→G L12L (TTA→TTG rimI → ribosomal‑protein‑S18‑alanine N‑acetyltransferase
RA 4,609,962 C→T Y183Y (TAC→TAT prfC → peptide chain release factor RF3
RA 4,621,903 A→G A45A (GCA→GCG yjjJ → toxin YjjJ
RA 4,629,055 A→G F490L (TTC→CTC)  ettA ← energy‑dependent translational throttle protein EttA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196273 1211433 15161 25 [21] [22] 26 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1633054–1633220 1633823–1633631 412–770 23 [22] [21] 23 ydfK–[pinQ] ydfK,[pinQ]
* * ÷ NC_000913 3423779–3424234 3424536–3424240 7–758 23 [21] [20] 23 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3941762–3942014 3946118–3945916 3903–4357 26 [22] [22] 23 rrsC–[rrlC] rrsC,gltU,[rrlC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)45 (0.820) 13/190 2.6 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375304 =NA (NA)26 (0.390) 19/232 2.2 NA noncoding (17/36 nt) other REP31h
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 = 12994980 (0.000)78 (1.000) 49/268 0.3 99.4% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 1 (0.010)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =103 (1.280)120 (1.510) 63/274 0.1 69.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)106 (1.320) 69/276 0.1 98.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA