Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F30 I1 R1	23	42.7	1455564	99.2%	1443919	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,293	G→T	F86L (TTC→TTA)	pgpC ←	phosphatidylglycerophosphatase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,293	0	G	T	100.0%	174.7 / NA	51	F86L (TTC→TTA)	pgpC	phosphatidylglycerophosphatase C
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (25/26); total (25/26)

TCGAAATAAACCGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCA  >  NC_000913/2698156‑2698426

tCGAAATAAACCGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTaa                                                                                                                                       >  2:287501/1‑140 (MQ=255)

 cGAAATAAACCGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTaaa                                                                                                                                      <  2:273039/140‑1 (MQ=255)

          ccGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCTTGc                                                                                                                             <  2:548279/140‑1 (MQ=255)

              ttCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACg                                                                                                                         <  2:511350/140‑1 (MQ=255)

                     aGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTaa                                                                                                                  >  2:96369/1‑140 (MQ=255)

                            gCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTTACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTCACATTGTCGCGAAACCAGCGCACTAAATCGGCATGCAACGTCTGTAAACGTGCt                                                                                                           <  2:710411/140‑1 (MQ=255)

                                    ccGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt‑gtg                                                                                                  >  1:574007/1‑140 (MQ=255)

                                    acGGTGATAAATCAGATATCGGCATCGGAGCTTAACGGGGAGGTGTGGAATCGCTCCTGAACCAGCGGGAAGGCGGTAACGTTGTCGCGAAACCAGGCCACTAAATCGGCCTGCAACGTCTGGAAACGAGCTTCGCt‑gtg                                                                                                  >  2:546015/2‑140 (MQ=255)

                                       gTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGAcc                                                                                               >  2:673565/1‑140 (MQ=255)

                                            cAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAg                                                                                          >  1:245516/1‑140 (MQ=255)

                                              aCCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTg                                                                                        <  1:223376/140‑1 (MQ=255)

                                              aCCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTg                                                                                        <  2:245516/140‑1 (MQ=255)

                                               ccAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGc                                                                                       >  2:317937/1‑140 (MQ=255)

                                                    tatCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCAcccc                                                                                  >  2:159673/1‑140 (MQ=255)

                                                      tCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCca                                                                                <  1:159673/140‑1 (MQ=255)

                                                             cGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGc                                                                        >  1:600491/1‑140 (MQ=255)

                                                             cGGAACTTAACAGGTAGGTGGATAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGc                                                                        >  2:597156/1‑140 (MQ=255)

                                                               gAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAg                                                                      <  2:574007/140‑1 (MQ=255)

                                                               gAACTTAACAGGTAGGTGGATAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAg                                                                      <  1:597156/140‑1 (MQ=255)

                                                                          gtaggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGcc                                                           >  1:660622/1‑140 (MQ=255)

                                                                             ggtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGc                                                        >  2:93127/1‑140 (MQ=255)

                                                                                 gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACTTTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGc                                                    >  2:709648/1‑140 (MQ=255)

                                                                                 gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGc                                                    >  2:458611/1‑140 (MQ=255)

                                                                                 gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGc                                                    >  2:40511/1‑140 (MQ=255)

                                                                                 gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACG‑GCGTCGCTGGGGACCAAAAGGGC‑CCCCCACAAAAAGAAACCTCACCGGCAAGCGtcc                                                    >  2:7246/1‑138 (MQ=255)

                                                                                     aTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCa                                                <  2:532370/140‑1 (MQ=255)

                                                                                       cGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACg                                              <  2:86536/140‑1 (MQ=255)

                                                                                        gCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGa                                             >  1:721928/1‑140 (MQ=255)

                                                                                        gCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGa                                             >  2:592234/1‑140 (MQ=255)

                                                                                         cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACgcc                                            <  1:546015/140‑3 (MQ=255)

                                                                                         cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGAc                                            >  2:303303/1‑140 (MQ=255)

                                                                                         cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGAc                                            <  2:612500/140‑1 (MQ=255)

                                                                                         cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGAc                                            <  1:233806/140‑1 (MQ=255)

                                                                                         cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGAc                                            <  1:96369/140‑1 (MQ=255)

                                                                                           ccTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCt                                          >  1:260691/1‑140 (MQ=255)

                                                                                              gAACCAGCGGAAAGGCGGTAACTTTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCtttt                                       <  1:709648/140‑1 (MQ=255)

                                                                                              gAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCtttt                                       <  1:458611/140‑1 (MQ=255)

                                                                                                    gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 <  2:303827/140‑1 (MQ=255)

                                                                                                    gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 >  1:279008/1‑140 (MQ=255)

                                                                                                    gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 >  1:573621/1‑140 (MQ=255)

                                                                                                    gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 >  1:303827/1‑140 (MQ=255)

                                                                                                    gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 <  1:40511/140‑1 (MQ=255)

                                                                                                      ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 >  2:302701/1‑138 (MQ=255)

                                                                                                      ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 <  1:302701/138‑1 (MQ=255)

                                                                                                      ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATaa                               >  2:572071/1‑140 (MQ=255)

                                                                                                          aGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCa                           <  2:419187/140‑1 (MQ=255)

                                                                                                          aGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCa                           <  2:260691/140‑1 (MQ=255)

                                                                                                          aGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCa                           <  2:660622/140‑1 (MQ=255)

                                                                                                          aGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGAATCATCGGCCAGCGTGCCGCACGCCCTTTTATCAATAACGCa                           <  2:234079/140‑1 (MQ=255)

                                                                                                           ggCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCaa                          <  1:673565/140‑1 (MQ=255)

                                                                                                                 aaCATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCta                    <  2:279008/140‑1 (MQ=255)

                                                                                                                        tCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCgg             >  2:331191/1‑140 (MQ=255)

                                                                                                                                 cAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACgg    <  2:721928/140‑1 (MQ=255)

                                                                                                                                 cAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACgg    <  2:600491/140‑1 (MQ=255)

                                                                                                                                   gcgcACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCa  <  1:287501/140‑1 (MQ=255)

TCGAAATAAACCGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCA  >  NC_000913/2698156‑2698426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A