breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,618 C→A P273Q (CCG→CAG)  thrB → homoserine kinase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 385,349 C→A Q40K (CAG→AAG)  tauA → taurine ABC transporter periplasmic binding protein
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,235,936 G→T S224S (TCG→TCT preA → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
RA 2,601,623 C→A T141T (ACC→ACA hyfA → hydrogenase 4 component A
RA 2,698,293 G→T F86L (TTC→TTA pgpC ← phosphatidylglycerophosphatase C
RA 3,037,861 G→T A82A (GCC→GCA recJ ← ssDNA‑specific exonuclease RecJ
RA 3,098,079 G→T V271V (GTG→GTT yggW → putative coproporphyrinogen‑III oxidase‑like protein YggW
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,715,561 G→T F278L (TTC→TTA bisC ← biotin sulfoxide reductase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,846,748 G→T G140G (GGC→GGA uhpT ← hexose‑6‑phosphate:phosphate antiporter
RA 3,847,131 G→T L13M (CTG→ATG)  uhpT ← hexose‑6‑phosphate:phosphate antiporter
RA 4,218,455 C→A intergenic (+42/‑141) aceA → / → aceK isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase
RA 4,221,548 C→A V314F (GTT→TTT)  arpA ← regulator of acetyl CoA synthetase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102974 3104076 1103 9 [7] [8] 9 mutY mutY
* * ÷ NC_000913 3423770–3424232 3424544–3424242 11–775 11 [8] [8] 10 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =56 (1.310)11 (0.290) 11/244 1.9 20.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 35 (0.930)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)49 (1.180) 41/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =61 (1.430)67 (1.580) 45/274 0.1 68.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)49 (1.150) 35/276 0.2 92.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.090)coding (3907/4134 nt) rhsA rhs element protein RhsA