Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A24 F30 I1 R1	17	36.3	1245384	98.8%	1230439	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,630,593	G→T	E90* (GAA→TAA)	yfgI →	nalidixic acid resistance protein YfgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,630,593	0	G	T	100.0%	140.5 / NA	40	E90* (GAA→TAA)	yfgI	nalidixic acid resistance protein YfgI
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (25/15); total (25/15)

CGAATGGGTCGCGGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCGAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAAC  >  NC_000913/2630460‑2630728

cGAATGGGTCGCGGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCct                                                                                                                                   >  1:84688/1‑140 (MQ=255)

         cgcgGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACa                                                                                                                          >  2:184166/1‑140 (MQ=255)

          gcgGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACaa                                                                                                                         >  1:231267/1‑140 (MQ=255)

          gcgGAGCACAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACaa                                                                                                                         >  1:511878/1‑140 (MQ=255)

           cgGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACaaa                                                                                                                        >  2:535349/1‑140 (MQ=255)

           cgGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACaaa                                                                                                                        >  1:93948/1‑140 (MQ=255)

                    aCAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGAt                                                                                                               <  2:301782/140‑1 (MQ=255)

                    aCAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGAt                                                                                                               <  2:231267/140‑1 (MQ=255)

                     cAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATc                                                                                                              >  2:431507/1‑140 (MQ=255)

                      aGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCa                                                                                                             <  2:84688/140‑1 (MQ=255)

                            aTCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTa                                                                                                       <  2:93948/140‑1 (MQ=255)

                                 gCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAg                                                                                                  <  2:408273/140‑1 (MQ=255)

                                  cAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGa                                                                                                 >  2:477732/1‑140 (MQ=255)

                                          gCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTAc                                                                                         <  2:478188/140‑1 (MQ=255)

                                           cAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        <  2:511878/140‑1 (MQ=255)

                                           cAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        <  1:431507/140‑1 (MQ=255)

                                           cAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        <  1:184166/140‑1 (MQ=255)

                                                        aTGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        >  2:271588/1‑127 (MQ=255)

                                                        aTGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACg                                                                                        <  1:271588/127‑1 (MQ=255)

                                                                    ccgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACaaaaa                                                               >  1:567573/1‑140 (MQ=255)

                                                                     cgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGTCGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAAc                                                              >  2:405437/1‑140 (MQ=255)

                                                                     cgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAAc                                                              >  1:77511/1‑140 (MQ=255)

                                                                     cgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAAc                                                              >  2:197318/1‑140 (MQ=255)

                                                                     cgcTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAAc                                                              >  1:240779/1‑140 (MQ=255)

                                                                          aCAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAActggcg                                                         <  1:477732/140‑1 (MQ=255)

                                                                          aCAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAActggcg                                                         <  1:138072/140‑1 (MQ=255)

                                                                                            cAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGcc                                       <  1:197318/140‑1 (MQ=255)

                                                                                                    aaTAAGAAAGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTaa                               >  1:105189/1‑140 (MQ=255)

                                                                                                            aGCTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGaaa                       >  1:400072/1‑140 (MQ=255)

                                                                                                              cTCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAAc                     >  1:126417/1‑140 (MQ=255)

                                                                                                               tCGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACg                    >  1:420307/1‑140 (MQ=255)

                                                                                                                cGTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGa                   >  1:371247/1‑140 (MQ=255)

                                                                                                                 gTCAGGTTAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGATAACGaa                  >  1:16462/1‑140 (MQ=255)

                                                                                                                 gTCAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGaa                  >  2:64506/1‑140 (MQ=255)

                                                                                                                   cAGGATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAAtt                <  1:535349/140‑1 (MQ=255)

                                                                                                                      gATAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTAt             <  2:77511/140‑1 (MQ=255)

                                                                                                                        tAAACTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCg           >  1:426886/1‑140 (MQ=255)

                                                                                                                           aCTGGACGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATc        >  1:428587/1‑140 (MQ=255)

                                                                                                                                aCGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGaa   >  2:82305/1‑140 (MQ=255)

                                                                                                                                 cGCCTAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAAc  >  2:339351/1‑140 (MQ=255)

CGAATGGGTCGCGGAGCGCAACAGGGAAATCCAGCAAGAGAAGCAACGTCGCGCAAATGCCCAGGCCGCCGCTAACAAAAGAGCGGCAACGGCAGCGGCAAATAAGAAAGCTCGTCAGGATAAACTGGACGCCGAAGCCTCTGCGGACAAAAAACGCGATCAAAGTTATGAAGATGAGCTACGCAGCTTAGAGATTCAGAAACAAAAACTGGCGCTGGCGAAAGAAGAAGCCCGCGTTAAGCGAGAAAACGAATTTATCGATCAGGAAC  >  NC_000913/2630460‑2630728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A