breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 905,528 G→T G206W (GGG→TGG)  amiD → N‑acetylmuramoyl‑L‑alanine amidase D
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,630,593 G→T E90* (GAA→TAA)  yfgI → nalidixic acid resistance protein YfgI
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,140,748 C→A A492S (GCC→TCC)  ptsA ← putative PTS multiphosphoryl transfer protein PtsA
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter
RA 4,568,243 C→A A93S (GCC→TCC)  mdtM ← multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102986 3104066 1081 7 [6] [6] 7 mutY mutY
* * ÷ NC_000913 3423785–3424233 3424525–3424240 8–741 9 [5] [5] 8 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 339959 =NA (NA)7 (0.240)
+28 bp
7/220 2.0 NA coding (167/297 nt) yahH putative uncharacterized protein YahH
?NC_000913 377433 = NA (NA)intergenic (+122/+102) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 374921 =NA (NA)57 (2.300) 14/190 0.6 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)66 (2.660) 23/190 0.1 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375001 =NA (NA)12 (0.420) 5/216 2.5 NA noncoding (17/36 nt) other REP31b
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 = 375059NA (NA)8 (0.220)
+T
8/274 2.3 NA noncoding (28/34 nt) other REP31c
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 = 375064NA (NA)16 (0.640) 9/192 1.3 NA noncoding (33/34 nt) other REP31c
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375102 =NA (NA)24 (0.850) 15/216 0.7 NA noncoding (17/36 nt) other REP31d
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 375124 =NA (NA)12 (0.480) 4/192 2.6 NA intergenic (+243/‑335) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377530 = NA (NA)intergenic (+219/+5) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375160NA (NA)14 (0.390)
+T
9/274 2.1 NA noncoding (28/34 nt) other REP31e
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 375203 =NA (NA)46 (1.630) 25/216 0.2 NA noncoding (17/36 nt) other REP31f
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 375248 =NA (NA)5 (0.160) 5/238 2.8 NA noncoding (15/34 nt) other REP31g
?NC_000913 377507 = NA (NA)noncoding (16/34 nt) other REP32d
* ? NC_000913 377353 =NA (NA)12 (0.420)
+30 bp
7/216 2.0 NA noncoding (18/37 nt) other REP32a
?NC_000913 4325850 = NA (NA)intergenic (‑109/+549) yjdN/yjdM conserved protein YjdN/conserved protein YjdM
* ? NC_000913 608007 =NA (NA)12 (0.410) 5/224 2.6 33.3% noncoding (1/1345 nt) IS186 repeat region
?NC_000913 609352 = 24 (0.820)intergenic (+175/+107) insL‑2/entD IS186/IS421 transposase/phosphopantetheinyl transferase EntD
* ? NC_000913 699424 =NA (NA)6 (0.220) 4/206 2.7 NA intergenic (‑247/+150) asnB/umpH asparagine synthetase B/UMP phosphatase
?NC_000913 = 2133430 NA (NA)intergenic (‑33/+60) wcaA/wzc putative colanic acid biosynthesis glycosyl transferase/protein‑tyrosine kinase Wzc
* ? NC_000913 = 1134685NA (NA)4 (0.150) 4/204 2.7 NA intergenic (+128/‑44) flgF/flgG flagellar basal‑body rod protein FlgF/flagellar basal‑body rod protein FlgG
?NC_000913 = 4326248 NA (NA)intergenic (‑507/+151) yjdN/yjdM conserved protein YjdN/conserved protein YjdM
* ? NC_000913 1207790 =11 (0.300)32 (1.000) 27/244 0.2 78.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 8 (0.250)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780511 (0.300)26 (0.810) 18/244 0.7 74.6% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 8 (0.250)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 2304714 =NA (NA)6 (0.190)
+GCAAGCGTCGCATCAGGCA
5/238 2.8 NA noncoding (17/36 nt) other REP161f
?NC_000913 = 4296059 NA (NA)intergenic (+265/+377) gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
* ? NC_000913 2518092 =34 (0.940)8 (0.250) 7/240 2.2 20.1% noncoding (25/76 nt) alaX tRNA‑Ala
?NC_000913 2521076 = 34 (1.080)noncoding (26/76 nt) valX tRNA‑Val
* ? NC_000913 3045988 =33 (0.910)35 (1.040) 7/256 2.4 59.5% intergenic (‑87/+180) ygfF/gcvP putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase
?NC_000913 3046089 = 17 (0.510)intergenic (‑188/+79) ygfF/gcvP putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase
* ? NC_000913 = 3281704NA (NA)15 (0.440) 8/262 2.2 NA noncoding (2/36 nt) other REP239a
?NC_000913 = 3281780 NA (NA)noncoding (5/34 nt) other REP239b
* ? NC_000913 3363001 =46 (1.270)70 (1.950) 42/274 0.0 75.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)46 (1.270) 33/276 0.1 92.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.110)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4373295 =NA (NA)15 (0.450) 9/256 1.9 NA noncoding (2/34 nt) other REP328b
?NC_000913 4374499 = NA (NA)noncoding (1/34 nt) other REP329a