Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F73 I1 R1	29	53.4	1802238	99.2%	1787820	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,092,290	G→T	A26D (GCC→GAC)	frvB ←	putative PTS enzyme IIBC component FrvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,092,290	0	G	T	100.0%	130.8 / NA	38	A26D (GCC→GAC)	frvB	putative PTS enzyme IIBC component FrvB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (18/20); total (18/20)

TCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGC  >  NC_000913/4092153‑4092426

tCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCt                                                                                                                                        >  2:888724/1‑140 (MQ=255)

tCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCt                                                                                                                                        >  2:714193/1‑140 (MQ=255)

    gCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCg                                                                                                                                    >  2:891896/1‑140 (MQ=255)

    gCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCg                                                                                                                                    >  1:891822/1‑140 (MQ=255)

    gCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCCGTTCCAGGTCTTCCg                                                                                                                                    >  1:409914/1‑140 (MQ=255)

     ccACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGc                                                                                                                                   <  2:873164/140‑1 (MQ=255)

     ccACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCCGTTCCAGGTCTTCCGc                                                                                                                                   <  2:409914/140‑1 (MQ=255)

            cGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATg                                                                                                                            <  1:596516/140‑1 (MQ=255)

             ggTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGt                                                                                                                           <  1:891896/140‑1 (MQ=255)

              gTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTa                                                                                                                          >  2:147916/1‑140 (MQ=255)

              gTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTa                                                                                                                          >  2:432153/1‑140 (MQ=255)

              gTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTa                                                                                                                          >  2:636504/1‑140 (MQ=255)

                           cGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATc                                                                                                             >  1:872006/1‑140 (MQ=255)

                                 cGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCcggcg                                                                                                       <  2:227425/140‑1 (MQ=255)

                                    cAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCgggg                                                                                                    <  2:351558/140‑1 (MQ=255)

                                    cAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCgggg                                                                                                    >  2:524147/1‑140 (MQ=255)

                                    cAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCgggg                                                                                                    <  1:195481/140‑1 (MQ=255)

                                    cAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCgggg                                                                                                    <  1:147916/140‑1 (MQ=255)

                                              ctctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGt                                                                                            >  2:84686/1‑138 (MQ=255)

                                              ctctTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGt                                                                                            <  1:84686/138‑1 (MQ=255)

                                                      tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACa                                                                                  <  2:379124/140‑1 (MQ=255)

                                                      tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACa                                                                                  <  1:432153/140‑1 (MQ=255)

                                                      tGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACa                                                                                  <  2:428802/140‑1 (MQ=255)

                                                       ggATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACaa                                                                                 <  2:373169/140‑1 (MQ=255)

                                                          tAAGCTGTTTTCGACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTGTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATAc                                                                              <  2:734483/140‑1 (MQ=255)

                                                                                 aCCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACt                                                       <  1:714193/140‑1 (MQ=255)

                                                                                     ttGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCt                                                   >  2:372629/1‑140 (MQ=255)

                                                                                      tGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCtt                                                  >  2:495410/1‑140 (MQ=255)

                                                                                       gAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTa                                                 <  2:891822/140‑1 (MQ=255)

                                                                                            tCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCCCTTCCTTATGAGc                                            >  1:893252/1‑140 (MQ=255)

                                                                                             ccACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCt                                           <  1:223642/140‑1 (MQ=255)

                                                                                                   ttATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCCTATGAGCTGAGGGt                                     <  1:636504/140‑1 (MQ=255)

                                                                                                                  gagaACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGc                      >  1:861241/1‑140 (MQ=255)

                                                                                                                   agaACGGGCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCa                     >  2:198525/1‑140 (MQ=255)

                                                                                                                        gggCTTTCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCAc                >  1:877060/1‑140 (MQ=255)

                                                                                                                             ttCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGt           <  2:659916/140‑1 (MQ=255)

                                                                                                                             ttCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGt           >  2:388351/1‑140 (MQ=255)

                                                                                                                             ttCTGTTCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGt           <  1:198525/140‑1 (MQ=255)

                                                                                                                                   tCCAGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTAt     <  2:669320/140‑1 (MQ=255)

                                                                                                                                      aGGTCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGc  <  2:872006/140‑1 (MQ=255)

TCAGGCCACGCCCGGTAGCGAGAATGACGTAATCGGCAGCGGCAATCTCTTCGCTGGATAAGCGGTTTTCAACGCCACTGGACCCTTGAGTTTCCACTTTTATGGTATGACCGAGAGAACGGGCTTTCTGTTCCAGGGCTTCCGCCACCATGTAGGTGTGAGCGATCCCGGCGGGGCAGTTGGTGATCGCGACAATACGTAAGGATGACTCCATCGCCACTTCCTTATGAGCTGAGGGTTTGATTTAACAAGGCAAGCACCTGGTCGGTATCGC  >  NC_000913/4092153‑4092426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A