Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F73 I1 R1 28 130.3 4467684 99.3% 4436410 139.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,030,318 C→A K218N (AAG→AAT)  fadB ← dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,030,3180CA100.0% 289.4 / NA 82K218N (AAG→AAT) fadBdodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (54/28);  total (54/28)

GGGCCGCAGCTTCAATGGTTTTTACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGCTTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGATCCGCGCC  >  NC_000913/4030185‑4030452
                                                                                                                                     |                                                                                                                                      
gggCCGCAGCTTCAATGGTTTTTACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGc                                                                                                                                  <  1:2157916/140‑1 (MQ=255)
  gCCGCAGCTTCAATGGTTTTTACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTg                                                                                                                                >  2:1007042/1‑140 (MQ=255)
    cGCAGCTTCAATGGTTTTTACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGAc                                                                                                                              >  1:455826/1‑140 (MQ=255)
    cGCAGCTTCAATGGTTTTTACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGAc                                                                                                                              >  1:1717230/1‑140 (MQ=255)
      cAGCTTCAATGGTTTTTACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGt                                                                                                                            >  1:899716/1‑140 (MQ=255)
       aGCTTCAATGGTTTTTACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGtt                                                                                                                           >  1:1858081/1‑140 (MQ=255)
          ttCAATGGTTTTTACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTg                                                                                                                        <  2:1858081/140‑1 (MQ=255)
                   ttttACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGt                                                                                                               >  2:1188427/1‑140 (MQ=255)
                   ttttACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGt                                                                                                               >  2:645450/1‑140 (MQ=255)
                    tttACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTc                                                                                                              >  1:2012646/1‑140 (MQ=255)
                       aCTGCGGAGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAg                                                                                                           >  1:1536245/1‑140 (MQ=255)
                        cTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAgg                                                                                                          <  1:1970948/140‑1 (MQ=255)
                        cTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAgg                                                                                                          <  2:899716/140‑1 (MQ=255)
                           cGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCg                                                                                                       >  2:1150845/1‑140 (MQ=255)
                           cGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCg                                                                                                       >  2:1039878/1‑140 (MQ=255)
                           cGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGCGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCg                                                                                                       >  2:1535964/1‑140 (MQ=255)
                            ggTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGc                                                                                                      <  1:1569260/140‑1 (MQ=255)
                            ggTGAT‑GGGGCCGGATAATGTTTCCCCGCTGTTTGTCCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGcc                                                                                                     >  1:1703758/1‑140 (MQ=255)
                                  gggggCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTaa                                                                                                >  1:979421/1‑140 (MQ=255)
                                     ggCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGTGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATgg                                                                                             >  1:550241/1‑140 (MQ=255)
                                     ggCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATgg                                                                                             >  1:824463/1‑140 (MQ=255)
                                       ccGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGcc                                                                                           <  1:2209387/140‑1 (MQ=255)
                                        cGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCt                                                                                          >  1:1963121/1‑140 (MQ=255)
                                        cGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGACCt                                                                                          <  2:2222356/140‑1 (MQ=255)
                                          gATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTgg                                                                                        <  2:1963121/140‑1 (MQ=255)
                                                  ttCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGcc                                                                                           >  1:1020900/1‑129 (MQ=255)
                                                  ttCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGcc                                                                                           <  2:1020900/129‑1 (MQ=255)
                                                    ccccGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCg                                                                              >  1:1804846/1‑140 (MQ=255)
                                                    ccccGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCg                                                                              >  1:363692/1‑140 (MQ=255)
                                                      ccGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGcc                                                                            >  2:918368/1‑140 (MQ=255)
                                                        gCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCtt                                                                          >  1:644384/1‑140 (MQ=255)
                                                          tGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTg                                                                        <  2:761476/140‑1 (MQ=255)
                                                          tGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTg                                                                        <  1:1039878/140‑1 (MQ=255)
                                                          tGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTg                                                                        >  2:1529108/1‑140 (MQ=255)
                                                          tGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTg                                                                        <  2:2012646/140‑1 (MQ=255)
                                                              tgtgCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGcc                                                                    >  2:1495574/1‑140 (MQ=255)
                                                              tgtgCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGcc                                                                    >  1:1318676/1‑140 (MQ=255)
                                                               gtgCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCt                                                                   <  1:648771/140‑1 (MQ=255)
                                                                tgCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCtt                                                                  <  1:92727/140‑1 (MQ=255)
                                                                 gCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTc                                                                 >  2:2129156/1‑140 (MQ=255)
                                                                  cGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCa                                                                <  1:1361958/140‑1 (MQ=255)
                                                                    aCCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAAc                                                              >  2:1752932/1‑140 (MQ=255)
                                                                    aCCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAAc                                                              >  2:2016801/1‑140 (MQ=255)
                                                                     ccATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAAcc                                                             <  2:841942/140‑1 (MQ=255)
                                                                      cATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCa                                                            >  2:766822/1‑140 (MQ=255)
                                                                        tCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGt                                                          >  1:71662/1‑140 (MQ=255)
                                                                        tCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGt                                                          >  1:2059225/1‑140 (MQ=255)
                                                                          ccTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCa                                                                <  2:1046173/132‑1 (MQ=255)
                                                                          ccTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCa                                                                >  1:1046173/1‑132 (MQ=255)
                                                                           cTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGtttt                                                       <  2:1804846/140‑1 (MQ=255)
                                                                             ttAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTc                                                     >  1:685568/1‑140 (MQ=255)
                                                                             ttAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTc                                                     <  1:1114691/140‑1 (MQ=255)
                                                                               aGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTTGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTg                                                   >  1:405528/1‑140 (MQ=255)
                                                                               aGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTg                                                   >  2:2076419/1‑140 (MQ=255)
                                                                                   aTGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCttt                                               >  2:66779/1‑140 (MQ=255)
                                                                                   aTGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCttt                                               >  2:1033167/1‑140 (MQ=255)
                                                                                   aTGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCttt                                               >  2:1858774/1‑140 (MQ=255)
                                                                                   aTGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGACGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCttt                                               >  2:19603/1‑140 (MQ=255)
                                                                                     ggTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGa                                             <  2:381177/140‑1 (MQ=255)
                                                                                     ggTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGa                                             <  1:1495574/140‑1 (MQ=255)
                                                                                     ggTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGa                                             >  2:1589255/1‑140 (MQ=255)
                                                                                      gTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGAc                                            >  1:1386039/1‑140 (MQ=255)
                                                                                              cATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCAt                                    >  1:1480412/1‑140 (MQ=255)
                                                                                              cATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCAt                                    >  2:2018726/1‑140 (MQ=255)
                                                                                                tggtggCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATcc                                  >  1:1147787/1‑140 (MQ=255)
                                                                                                tggtggCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATcc                                  >  2:2137396/1‑140 (MQ=255)
                                                                                                 ggtggCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATcca                                 >  2:598871/1‑140 (MQ=255)
                                                                                                  gtggCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATccac                                <  2:550241/140‑1 (MQ=255)
                                                                                                      cTTCAATCTTGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGa                            >  1:1794224/1‑140 (MQ=255)
                                                                                                               tGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTc                   <  2:986690/140‑1 (MQ=255)
                                                                                                               tGCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACAGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTc                   <  1:830856/140‑1 (MQ=255)
                                                                                                                gCTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCa                  <  1:2018726/140‑1 (MQ=255)
                                                                                                                 cTCAGTTTTAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAg                 >  1:1157212/1‑140 (MQ=255)
                                                                                                                      ttttAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCt            >  2:66876/1‑140 (MQ=255)
                                                                                                                         tAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGAt         <  1:645450/140‑1 (MQ=255)
                                                                                                                         tAGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGAt         >  1:1615387/1‑140 (MQ=255)
                                                                                                                          aGTGGTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGATc        <  2:644384/140‑1 (MQ=255)
                                                                                                                              gTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGATCcgcg    >  1:780690/1‑140 (MQ=255)
                                                                                                                              gTTCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGATCcgcg    <  1:1858774/140‑1 (MQ=255)
                                                                                                                                ttcAGATTCGGCGGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCACCCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGATCCGGGcc  >  2:142725/3‑140 (MQ=255)
                                                                                                                                tCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGATCCGCGcc  >  2:1689808/1‑140 (MQ=255)
                                                                                                                                tCCAGATTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGATCCGCGcc  >  1:935045/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                      
GGGCCGCAGCTTCAATGGTTTTTACTGCGGTGATGGGGGCCGGATAATGTTTCCCCGCTGTTTGTGCGACCATCCCTTTAGCGATGGTGAAGCTCATGGTGGCTTCAATCTTGCTCAGTTTTAGTGGTTCCAGCTTCGGCTGACGTTTTGCTTTCCAGTCGAGGTCGCCGTTAATGGCCTGGCGTAAAACCGCCTTTGCGCCTTCAACCAGTTTTTCTGCTTTGACTACGCCATCCACCAGACCGATTTTCAGCGCCTGATCCGCGCC  >  NC_000913/4030185‑4030452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A