breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 38,805 C→A R104L (CGT→CTT)  caiB ← gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 369,465 C→A F285L (TTC→TTA mhpA → putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase
RA 527,694 (A)5→4 coding (114/369 nt) ybbC → PF15631 family protein YbbC
RA 578,333 A→C K76T (AAA→ACA) 
K3Q (AAG→CAG) 
rzpD →
rzoD →
DLP12 prophage; putative prophage endopeptidase RzpD
DLP12 prophage; putative prophage lysis lipoprotein RzoD
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,087,644 G→A A68V (GCT→GTT)  pgaC ← poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaC
RA 1,404,886 C→G A49G (GCG→GGG)  abgR → putative LysR‑type DNA‑binding transcriptional regulator AbgR
RA 1,432,529 C→A A40E (GCA→GAA)  tfaR → Rac prophage; putative tail fiber assembly protein TfaR
RA 1,437,374 C→A S1137S (TCG→TCT ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,649,933 C→A P47Q (CCG→CAG)  ydfD → Qin prophage; lysis protein
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,021,557 G→A L18L (CTG→CTA fliO → flagellar biosynthesis protein FliO
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,511,277 G→T D93Y (GAT→TAT)  ypeC → DUF2502 domain‑containing protein YpeC
RA 3,147,848 G→T intergenic (‑108/‑49) yqhI ← / → gpr protein YqhI/L‑glyceraldehyde 3‑phosphate reductase
RA 3,353,199 G→T V284V (GTC→GTA yhcC ← radical SAM family oxidoreductase YhcC
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,030,318 C→A K218N (AAG→AAT fadB ← dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
RA 4,182,494 C→A S417Y (TCC→TAC)  rpoB → RNA polymerase subunit beta
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,348,011 G→T A245E (GCA→GAA)  dcuB ← anaerobic C4‑dicarboxylate transporter DcuB
RA 4,631,944 C→A S404R (AGC→AGA slt → soluble lytic murein transglycosylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102962 3104052 1091 38 [36] [35] 37 [mutY] [mutY]
* * ÷ NC_000913 3423751–3424540 3424540 1–790 37 [36] [35] 37 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =47 (0.360)151 (1.290) 67/244 0.2 80.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 30 (0.260)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780547 (0.360)64 (0.550) 46/244 0.9 64.1% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 30 (0.260)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)170 (1.330) 87/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =207 (1.570)215 (1.640) 77/274 0.2 67.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)163 (1.230) 80/276 0.1 95.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 7 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA