Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F73 I1 R1	30	41.4	1413620	99.2%	1402311	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,091,937	G→A	V281I (GTT→ATT)	hisD →	histidinal/histidinol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,091,937	0	G	A	100.0%	105.2 / NA	31	V281I (GTT→ATT)	hisD	histidinal/histidinol dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/13); total (18/13)

CGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGA  >  NC_000913/2091803‑2092073

cGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGcc                                                                                                                                     >  2:189221/1‑140 (MQ=255)

                      tGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTgg                                                                                                               <  2:40321/140‑1 (MQ=255)

                          aGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGa                                                                                                           >  2:128810/1‑140 (MQ=255)

                           gcggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCa                                                                                                                    >  2:243277/1‑130 (MQ=255)

                           gcggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCa                                                                                                                    <  1:243277/130‑1 (MQ=255)

                            cggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAAc                                                                                                         >  1:70528/1‑140 (MQ=255)

                               cgcTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGc                                                                                                      >  2:361725/1‑140 (MQ=255)

                                               gTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAAccgc                                                                                      <  1:13189/140‑1 (MQ=255)

                                                             gCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGa                                                                        >  1:233815/1‑140 (MQ=255)

                                                             gCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGa                                                                        >  1:660873/1‑140 (MQ=255)

                                                              ctctctCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGaa                                                                       <  1:361725/140‑1 (MQ=255)

                                                                           aaCACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAgccgc                                                             >  2:502918/1‑137 (MQ=255)

                                                                           aaCACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAgccgc                                                             <  1:502918/137‑1 (MQ=255)

                                                                                  cccGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGa                                                   >  1:603123/1‑140 (MQ=255)

                                                                                     ggACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTa                                                >  2:61843/1‑140 (MQ=255)

                                                                                      gACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTaa                                               <  2:168153/140‑1 (MQ=255)

                                                                                       aCTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTaaa                                              >  1:376054/1‑140 (MQ=255)

                                                                                           acaGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGAtt                                          >  2:350480/1‑140 (MQ=255)

                                                                                            caGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCACATTGCCGAGGCCGTCGAACGACAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGAttt                                         >  2:646039/1‑140 (MQ=255)

                                                                                               gTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAgc                                      >  2:636132/1‑140 (MQ=255)

                                                                                                 gATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAgcgc                                    <  1:189221/140‑1 (MQ=255)

                                                                                                  aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  2:83364/140‑1 (MQ=255)

                                                                                                  aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  2:660873/140‑1 (MQ=255)

                                                                                                  aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  1:128810/140‑1 (MQ=255)

                                                                                                  aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  2:148086/140‑1 (MQ=255)

                                                                                                  aTTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCa                                   <  1:299595/140‑1 (MQ=255)

                                                                                                           aCGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGga                          <  2:70528/140‑1 (MQ=255)

                                                                                                            cGCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGgag                         >  1:54652/1‑140 (MQ=255)

                                                                                                             gCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGgaga                        >  2:420847/1‑140 (MQ=255)

                                                                                                             gCCCGCTGCTGATATGGCGCGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGgaga                        >  1:319759/1‑140 (MQ=255)

                                                                                                                             gcgcGTCGCATTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACgg        >  1:187571/1‑140 (MQ=255)

                                                                                                                                 gtcgcatTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCg    <  1:420847/140‑1 (MQ=255)

                                                                                                                                  tcgcatTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCgg   >  2:187585/1‑140 (MQ=255)

                                                                                                                                  tcgcatTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCgg   <  2:501335/140‑1 (MQ=255)

                                                                                                                                   cgcatTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGa  >  2:148759/1‑140 (MQ=255)

CGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAACGCCAACTGGCAGAACTGCCGCGTGCCGAAACCGCCCGCCAGGCACTGAACGCCAGCCGCCTGATCGTGACTAAAGATTTAGCGCAGTGCGTGGAGATCTCCAACCAGTACGGCCCGGA  >  NC_000913/2091803‑2092073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A