Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F73 I1 R1	30	41.4	1413620	99.2%	1402311	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,340,494	C→A	L74I (CTC→ATC)	yrbG →	putative transport protein YrbG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,340,494	0	C	A	100.0%	190.9 / NA	54	L74I (CTC→ATC)	yrbG	putative transport protein YrbG
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (21/33); total (21/33)

TGCCGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTC  >  NC_000913/3340362‑3340624

tGCCGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTc                                                                                                                             >  1:165950/1‑140 (MQ=255)

tGCCGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTc                                                                                                                             >  1:698887/1‑140 (MQ=255)

  ccGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCaa                                                                                                                           <  1:408092/140‑1 (MQ=255)

    gAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAAc                                                                                                                         <  1:235310/140‑1 (MQ=255)

    gAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAAc                                                                                                                         <  2:470382/140‑1 (MQ=255)

    gAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAAc                                                                                                                         <  1:673381/140‑1 (MQ=255)

       ccTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACAtt                                                                                                                      <  1:63776/140‑1 (MQ=255)

         tttGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTAt                                                                                                                    <  2:472360/140‑1 (MQ=255)

           tggccgcccGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  <  2:414751/134‑1 (MQ=255)

           tGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  <  1:208818/140‑1 (MQ=255)

           tGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  >  2:194914/1‑140 (MQ=255)

                 cccGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAatatat                                                                                                            >  1:678530/1‑140 (MQ=255)

                   cgccgcTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTg                                                                                                          >  2:631081/1‑140 (MQ=255)

                    gccgcTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGc                                                                                                         <  1:587668/140‑1 (MQ=255)

                     ccgcTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  <  2:524799/130‑1 (MQ=255)

                     ccgcTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCa                                                                                                                  >  1:524799/1‑130 (MQ=255)

                         tGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATc                                                                                                    <  1:631081/140‑1 (MQ=255)

                              atcGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGTGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCgg                                                                                               <  2:148347/140‑1 (MQ=255)

                                cGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTc                                                                                             >  1:409347/1‑140 (MQ=255)

                                     tGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCt                                                                                        >  2:440011/1‑140 (MQ=255)

                                       aCGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTgc                                                                                      >  1:604964/1‑140 (MQ=255)

                                       aCGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTgc                                                                                      >  1:65765/1‑140 (MQ=255)

                                              tCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGtt                                                                               <  1:442388/140‑1 (MQ=255)

                                                       gTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCtttt                                                                      >  1:92984/1‑140 (MQ=255)

                                                        tACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTa                                                                     <  1:190152/140‑1 (MQ=255)

                                                            tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCg                                                                             <  1:652003/128‑1 (MQ=255)

                                                            tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCg                                                                             >  2:652003/1‑128 (MQ=255)

                                                            tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 >  1:483114/1‑140 (MQ=255)

                                                            tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 <  1:475719/140‑1 (MQ=255)

                                                            tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 <  1:142773/140‑1 (MQ=255)

                                                            tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 >  1:28311/1‑140 (MQ=255)

                                                            tCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGt                                                                 >  2:142773/1‑140 (MQ=255)

                                                                tACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCATCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCAt                                                             <  2:604964/140‑1 (MQ=255)

                                                                    aGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTg                                                         <  2:491055/140‑1 (MQ=255)

                                                                           aTCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCt                                                  >  2:385228/1‑140 (MQ=255)

                                                                                ctcGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGcc                                             <  1:385228/140‑1 (MQ=255)

                                                                                ctcGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGcc                                             <  1:194914/140‑1 (MQ=255)

                                                                                ctcGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGcc                                             <  2:196884/140‑1 (MQ=255)

                                                                                      tGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAAt                                       <  2:409347/140‑1 (MQ=255)

                                                                                      tGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAAt                                       <  1:199527/140‑1 (MQ=255)

                                                                                          gcgTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTAcc                                   <  2:521065/140‑1 (MQ=255)

                                                                                          gcgTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTAcc                                   <  1:622601/140‑1 (MQ=255)

                                                                                          gcgTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTAcc                                   <  2:165950/140‑1 (MQ=255)

                                                                                             tctcTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCtt                                <  2:632473/140‑1 (MQ=255)

                                                                                                 tGCACGAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAAtg                            >  1:248284/1‑140 (MQ=255)

                                                                                                      gAACAACGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATgttgtt                       <  2:678530/140‑1 (MQ=255)

                                                                                                        acaacGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTgg                     >  1:702536/1‑140 (MQ=255)

                                                                                                           acGCGATTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCa                  >  1:674639/1‑140 (MQ=255)

                                                                                                                aTTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCgtg             <  2:577949/140‑1 (MQ=255)

                                                                                                                aTTTAGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCgtg             <  1:440011/140‑1 (MQ=255)

                                                                                                                    aGCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGtggtgg         >  1:247049/1‑140 (MQ=255)

                                                                                                                     gCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGc        <  1:260194/140‑1 (MQ=255)

                                                                                                                     gCCGTTGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGc        <  2:488685/140‑1 (MQ=255)

                                                                                                                          tGGTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGtt   <  1:425503/140‑1 (MQ=255)

                                                                                                                           ggTACAGCCATCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTc  >  1:406626/1‑140 (MQ=255)

TGCCGAACCTTTGGCATCCCGCCGCTGATCATCGGCATGACGGTGGTCAGTATTGGTACATCGTTACCAGAAGTCATCGTCTCGCTTGCCGCGTCTCTGCACGAACAACGCGATTTAGCCGTTGGTACAGCCCTCGGCTCAAACATTATCAATATATTGCTGATCCTCGGTCTGGCTGCGCTGGTTCGTCCTTTTACCGTCCATTCTGATGTTCTACGCCGTGAATTACCCTTAATGTTGTTGGTCAGCGTGGTGGCCGGTTC  >  NC_000913/3340362‑3340624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A