Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F73 I1 R1 31 34.8 1203930 99.1% 1193094 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,226,578 C→A F115L (TTC→TTA)  ygjI → putative transporter YgjI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,226,5780CA100.0% 143.2 / NA 42F115L (TTC→TTA) ygjIputative transporter YgjI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/27);  total (15/27)

CGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTG  >  NC_000913/3226443‑3226710
                                                                                                                                        |                                                                                                                                    
cGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCg                                                                                                                                  <  2:110459/140‑1 (MQ=255)
cGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCg                                                                                                                                  <  1:350958/140‑1 (MQ=255)
 ggTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCgg                                                                                                                                 >  1:60772/1‑140 (MQ=255)
  gtgtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGa                                                                                                                                <  2:387103/140‑1 (MQ=255)
  gtgtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGa                                                                                                                                <  1:328560/140‑1 (MQ=255)
  gtgtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGa                                                                                                                                >  1:418890/1‑140 (MQ=255)
   tgtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGAt                                                                                                                               <  2:85598/140‑1 (MQ=255)
    gtCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATa                                                                                                                              <  1:305166/140‑1 (MQ=255)
     tCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATAc                                                                                                                             <  2:23906/140‑1 (MQ=255)
       tACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGa                                                                                                                           <  2:87779/140‑1 (MQ=255)
        aCGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGaa                                                                                                                          <  1:50257/140‑1 (MQ=255)
               ggTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATAc                                                                                                                             >  1:451873/1‑130 (MQ=255)
               ggTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATAc                                                                                                                             <  2:451873/130‑1 (MQ=255)
                 tAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATg                                                                                                                 >  1:134482/1‑140 (MQ=255)
                              gggCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGcca                                                                                                    <  2:574218/140‑1 (MQ=255)
                              gggCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGcca                                                                                                    <  2:322352/140‑1 (MQ=255)
                              gggCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGATGcca                                                                                                    <  2:283101/140‑1 (MQ=255)
                                gCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGccacc                                                                                                  <  1:548556/140‑1 (MQ=255)
                                   gACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGccaccacc                                                                                               <  1:562236/140‑1 (MQ=255)
                                        tGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAt                                                                                          <  1:344228/140‑1 (MQ=255)
                                        tGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAt                                                                                          <  1:198041/140‑1 (MQ=255)
                                        tGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAt                                                                                          <  1:179500/140‑1 (MQ=255)
                                         gggCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTCTTTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTAt                                                                                          <  1:326367/140‑1 (MQ=255)
                                         gggCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGccaccacc                                                                                               >  1:413254/1‑134 (MQ=255)
                                         gggCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGccaccacc                                                                                               <  2:413254/134‑1 (MQ=255)
                                                tattaCTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATgg                                                                                  <  2:558485/140‑1 (MQ=255)
                                                        ccTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTc                                                                          >  2:128011/1‑140 (MQ=255)
                                                         cTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCg                                                                         <  1:146916/140‑1 (MQ=255)
                                                                  cTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCa                                                                >  1:249244/1‑140 (MQ=255)
                                                                       tCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTgg                                                           >  1:360251/1‑140 (MQ=255)
                                                                                  ttC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCaa                                                >  2:541415/1‑140 (MQ=255)
                                                                                                cACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATg                                   >  1:155189/1‑140 (MQ=255)
                                                                                                 aCTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGt                                  <  1:597112/140‑1 (MQ=255)
                                                                                                      tGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTgggg                             <  1:64349/140‑1 (MQ=255)
                                                                                                           cgcgTTATTGCTTATGCTTCGTATGCCTTACTCGGATACG‑‑‑ATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTAc                     >  1:437062/1‑140 (MQ=255)
                                                                                                             cgTTATTGCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTa                      >  1:210910/1‑140 (MQ=255)
                                                                                                                    gCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGt               <  2:134482/140‑1 (MQ=255)
                                                                                                                    gCTTATGCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGt               <  2:258688/140‑1 (MQ=255)
                                                                                                                          gCTTCGTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTc         >  2:498904/1‑140 (MQ=255)
                                                                                                                               gTATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGc    >  1:338096/1‑140 (MQ=255)
                                                                                                                                tATGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCt   >  2:219171/1‑140 (MQ=255)
                                                                                                                                 aTGCCTTACTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTg  <  1:88135/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CGGTGTCTACGCGTGGGTAAAAAGTTCGCTGGGCGGACGTTGGGCATTTATTACTGCCTATACCTACTGGTTCGTAAACCTGTTC‑TTTTTCACCTCACTGTTGCCGCGCGTTATTGCTTATGCTTCGTATGCCTTCCTCGGATACGAATATATTATGACGCCGGTTGCCACCACCATTATCAGTATGGTGCTGTTCGCCTTCTCCACCTGGGTTTCCACCAACGGGGCGAAAATGTTGGGGCCAATTACCTCCGTCACTTCAACGCTG  >  NC_000913/3226443‑3226710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A