Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F73 I1 R1
|
26 |
44.8 |
1518598 |
99.2% |
1506449 |
138.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,411,207 |
G→A |
A219T (GCC→ACC) |
yjfJ → |
putative transcriptional regulator effector protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,411,207 | 0 | G | A | 92.3%
| 65.8
/ ‑0.8
| 26 | A219T (GCC→ACC) | yjfJ | putative transcriptional regulator effector protein |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (7/17); total (8/18) |
| Fisher's exact test for biased strand distribution p-value = 5.29e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.94e-01 |
TAAAACGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCGCCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAA > NC_000913/4411070‑4411333
|
tAAAACGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCAcc < 1:639476/140‑1 (MQ=255)
aaCGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAg < 1:668727/140‑1 (MQ=255)
aCGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAgg < 1:153766/140‑1 (MQ=255)
aCGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAgg < 2:108796/140‑1 (MQ=255)
gCCTGCAAACGCGTCAGGCCGAACGTCAGGCTAGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGAt < 1:182862/140‑1 (MQ=255)
ccTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGGAGCACCCAGGATg > 2:289407/1‑140 (MQ=255)
cTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGt > 2:514058/1‑140 (MQ=255)
aaCGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAg < 1:484547/140‑1 (MQ=255)
ggCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGc < 1:26262/140‑1 (MQ=255)
ccGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCaa < 1:514058/140‑1 (MQ=255)
ccTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGt > 1:469730/1‑140 (MQ=255)
tgcCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCGCCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAAttttttt > 1:322181/1‑140 (MQ=255)
ccGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGc > 1:343898/1‑140 (MQ=255)
acaGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGc < 2:343898/140‑1 (MQ=255)
ttGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCg > 1:709695/1‑140 (MQ=255)
gaAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTaaa < 2:709695/140‑1 (MQ=255)
gaAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTaaa < 2:351130/140‑1 (MQ=255)
tCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTaa > 1:290481/1‑134 (MQ=255)
tCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTaa < 2:290481/134‑1 (MQ=255)
tCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCt < 1:358589/140‑1 (MQ=255)
tCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAAGAGGACCCAGGGTGTATTAGCAAGACTGCAACGGCAAAAAGGGGAGGAATTTTTTTTGCCGCCTCGCTAAAAgggt > 2:524181/1‑138 (MQ=255)
cAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGc < 2:469730/140‑1 (MQ=255)
aGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCa < 2:74511/140‑1 (MQ=255)
aaaaGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGATAAAata > 1:517825/1‑140 (MQ=255)
gCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGAt < 2:506934/126‑1 (MQ=255)
gCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGAt > 1:506934/1‑126 (MQ=255)
aGCAATAAAAGTAGCACCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAGGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGc < 1:524181/140‑1 (MQ=255)
cAATAAAAGTAGCGCCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCaa < 2:322181/140‑1 (MQ=255)
|
TAAAACGCCTGCAAACGCGTCAGGCCGAACGTCAGGCTCGCCTGGATGCTGCCGCACAGTTGGAGAAAGTCGCAGACGGTCGCGACCTTGACGAAAAGCTGGCGGAAGCCGGAATTGGCGGTAGCAATAAAAGTAGCGCCCAGGATGTATTAGCAAGACTGCAACGCCAACAGGGCGAGTAATTTTTTTGCCAGCCTCGCTAAAAGGCTGGCAACTATTTTAAGGATAAAATATGTCTGGTTTTTTCCAGCGTCTGTTTGGCAA > NC_000913/4411070‑4411333
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
