Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 172 67.8 2301510 98.9% 2276193 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,137,202 T→C T441A (ACC→GCC)  gss ← fused glutathionylspermidine amidase/glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,137,2020TC100.0% 200.7 / NA 57T441A (ACC→GCC) gssfused glutathionylspermidine amidase/glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/35);  total (22/35)

ACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCAT  >  NC_000913/3137066‑3137328
                                                                                                                                        |                                                                                                                              
aCCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGcttt                                                                                                                             <  1:418177/140‑1 (MQ=255)
    gCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcac                                                                                                                         <  1:837864/140‑1 (MQ=255)
     cACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        <  2:404736/140‑1 (MQ=255)
     cACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        <  1:691329/140‑1 (MQ=255)
       cTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACg                                                                                                                      <  1:337478/140‑1 (MQ=255)
          ccGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        <  2:162356/135‑1 (MQ=255)
          ccGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        >  1:162356/1‑135 (MQ=255)
             ggCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        <  1:840212/132‑1 (MQ=255)
             ggCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCcaca                                                                                                                        >  2:840212/1‑132 (MQ=255)
             ggCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTa                                                                                                                <  1:406793/140‑1 (MQ=255)
               cgcAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACg                                                                                                                      >  2:866354/1‑132 (MQ=255)
               cgcAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACg                                                                                                                      <  1:866354/132‑1 (MQ=255)
               cgcAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAAc                                                                                                              >  2:829200/1‑140 (MQ=255)
                   gcaATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGt                                                                                                          <  1:556045/140‑1 (MQ=255)
                   gcaATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGt                                                                                                          <  2:786939/140‑1 (MQ=255)
                     aaTACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCg                                                                                                        <  2:833412/140‑1 (MQ=255)
                      aTACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGc                                                                                                       <  2:684033/140‑1 (MQ=255)
                      aTACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGc                                                                                                       <  1:1110232/140‑1 (MQ=255)
                          gTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCtt                                                                                                   <  1:763895/140‑1 (MQ=255)
                             gATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTccc                                                                                                <  2:682407/140‑1 (MQ=255)
                              aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGc                                                                                                       >  1:903601/1‑132 (MQ=255)
                              aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGc                                                                                                       <  2:903601/132‑1 (MQ=255)
                              aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCtt                                                                                                   <  2:1066971/136‑1 (MQ=255)
                              aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCtt                                                                                                   >  1:1066971/1‑136 (MQ=255)
                              aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTcccc                                                                                               >  2:731280/1‑140 (MQ=255)
                              aTAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTcccc                                                                                               >  2:1019968/1‑140 (MQ=255)
                                 aGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCatc                                                                                            >  1:570152/1‑140 (MQ=255)
                                     gCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCatcaatc                                                                                        <  1:550579/140‑1 (MQ=255)
                                      cACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCatcaatca                                                                                       >  1:1147531/1‑140 (MQ=255)
                                       aCTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAg                                                                                      <  1:872929/140‑1 (MQ=255)
                                       aCTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAg                                                                                      <  1:16859/140‑1 (MQ=255)
                                         ttCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGtt                                                                                    >  2:1086882/1‑140 (MQ=255)
                                                  cGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGgcag                                                                           <  2:432552/140‑1 (MQ=255)
                                                      tGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATc                                                                       <  1:449455/140‑1 (MQ=255)
                                                          cGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccag                                                                   <  1:731280/140‑1 (MQ=255)
                                                           ggTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagc                                                                  <  1:1086882/140‑1 (MQ=255)
                                                                    tGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCat                                                         >  1:434847/1‑140 (MQ=255)
                                                                    tGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCat                                                         <  2:460225/140‑1 (MQ=255)
                                                                       cACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatcca                                                      >  1:973153/1‑140 (MQ=255)
                                                                          cGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaatc                                                   <  2:1116277/140‑1 (MQ=255)
                                                                            cagcaAACTCACTGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaatcca                                                 >  1:750709/1‑140 (MQ=255)
                                                                              gcaAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACg                                               <  1:829200/140‑1 (MQ=255)
                                                                                    tCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGAt                                         >  2:633199/1‑140 (MQ=255)
                                                                                         gTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATAcgcg                                    >  2:719947/1‑140 (MQ=255)
                                                                                                  ttCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGc                           <  1:1019968/140‑1 (MQ=255)
                                                                                                  ttCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGc                           <  1:324983/140‑1 (MQ=255)
                                                                                                       gAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGcc                      <  2:434847/140‑1 (MQ=255)
                                                                                                       gAATCTGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGcc                      <  1:1039396/140‑1 (MQ=255)
                                                                                                            tGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTg                 >  2:393583/1‑140 (MQ=255)
                                                                                                            tGATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCACCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTg                 >  2:1012883/1‑140 (MQ=255)
                                                                                                             gATCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGc                <  1:393583/140‑1 (MQ=255)
                                                                                                              aTCAAACGCGGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCa               >  1:188250/1‑140 (MQ=255)
                                                                                                                    cgcgGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCt         <  2:269612/140‑1 (MQ=255)
                                                                                                                     gcgGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTg        <  2:1147531/140‑1 (MQ=255)
                                                                                                                      cgGTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCACCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGc       <  1:1012883/140‑1 (MQ=255)
                                                                                                                        gTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTc     >  1:677984/1‑140 (MQ=255)
                                                                                                                        gTTTCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGCGCAGCGCCTGCTc     >  2:952977/1‑140 (MQ=255)
                                                                                                                           tCCCACGCCCAGGCTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCAt  >  1:934904/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                              
ACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCCGCCTGGTGCAGCGCCTGCTCCAT  >  NC_000913/3137066‑3137328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A