Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 172 67.8 2301510 98.9% 2276193 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,222,167 T→C Y234Y (TAT→TAC)  ebgR → DNA‑binding transcriptional repressor EbgR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,222,1670TC100.0% 165.3 / NA 48Y234Y (TAT→TAC) ebgRDNA‑binding transcriptional repressor EbgR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/26);  total (22/26)

GCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTT  >  NC_000913/3222030‑3222302
                                                                                                                                         |                                                                                                                                       
gCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTAccc                                                                                                                                       >  2:848783/1‑140 (MQ=255)
       ttCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCa                                                                                                                                <  2:163010/140‑1 (MQ=255)
           tGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACtgt                                                                                                                            >  1:774933/1‑140 (MQ=255)
                 cGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCg                                                                                                                                      >  2:877551/1‑124 (MQ=255)
                 cGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCg                                                                                                                                      <  1:877551/124‑1 (MQ=255)
                  gCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAgg                                                                                                                                  <  1:110352/127‑1 (MQ=255)
                  gCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAgg                                                                                                                                  >  2:110352/1‑127 (MQ=255)
                  gCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGc                                                                                                                     >  2:352398/1‑140 (MQ=255)
                   ccTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCt                                                                                                                    <  1:45954/140‑1 (MQ=255)
                             atatGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCa                                                                                                          <  1:588339/140‑1 (MQ=255)
                                      aCTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTa                                                                                                    <  1:932928/137‑1 (MQ=255)
                                      aCTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTa                                                                                                    >  2:932928/1‑137 (MQ=255)
                                        tGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGc                                                                                               <  1:835498/140‑1 (MQ=255)
                                              aaGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTg                                                                                         <  1:690172/140‑1 (MQ=255)
                                                 tGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCgg                                                                                      >  1:378406/1‑140 (MQ=255)
                                                  ggTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCggg                                                                                     <  1:352398/140‑1 (MQ=255)
                                                   gTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCggg                                                                                     >  2:319539/1‑139 (MQ=255)
                                                   gTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCggg                                                                                     <  1:319539/139‑1 (MQ=255)
                                                      cgcgAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCTGCGGGGTATGAACTGGCAAAAGTAATGCTGGCTCGGGGAACCTTGCCCGAGGCAACGTTTGTTGCTTCCCCTTTCATTGCTATCGACGTAGTGCggcgaat                                                                                 >  2:75187/1‑135 (MQ=255)
                                                              ggATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                                         <  2:1138959/140‑1 (MQ=255)
                                                              ggATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                                         <  1:1001244/140‑1 (MQ=255)
                                                                 tatCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAg                                                                      <  2:378406/140‑1 (MQ=255)
                                                                   tCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGc                                                                    >  1:679519/1‑140 (MQ=255)
                                                                   tCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGc                                                                    >  1:880651/1‑140 (MQ=255)
                                                                    cTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGcc                                                                   >  1:148178/1‑140 (MQ=255)
                                                                       gcgcGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGa                                                                >  1:509414/1‑140 (MQ=255)
                                                                        tgcggcgGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGaa                                                               <  1:75187/139‑1 (MQ=255)
                                                                          cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa                                                             <  2:509414/140‑1 (MQ=255)
                                                                          cggcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACa                                                             <  2:148178/140‑1 (MQ=255)
                                                                            gcggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATc                                                           >  2:490323/1‑140 (MQ=255)
                                                                             cggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                                         <  2:366249/125‑1 (MQ=255)
                                                                             cggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAAc                                                                         >  1:366249/1‑125 (MQ=255)
                                                                              ggTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATccc                                                         <  1:490323/140‑1 (MQ=255)
                                                                                tttttCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCcac                                                       <  1:426522/140‑1 (MQ=255)
                                                                                      cAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCAGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGata                                                 <  2:807611/140‑1 (MQ=255)
                                                                                              cgGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCtt                                         >  2:955569/1‑140 (MQ=255)
                                                                                                          tGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                             <  1:955569/140‑1 (MQ=255)
                                                                                                          tGGCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAAc                             <  1:848783/140‑1 (MQ=255)
                                                                                                            gCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGtt                                >  1:503215/1‑135 (MQ=255)
                                                                                                            gCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGtt                                <  2:503215/135‑1 (MQ=255)
                                                                                                            gCAAAACAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                           >  2:203673/1‑140 (MQ=255)
                                                                                                                 aCAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCa                                     >  2:336155/1‑125 (MQ=255)
                                                                                                                 aCAAATGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCa                                     <  1:336155/125‑1 (MQ=255)
                                                                                                                      tGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                           >  2:99615/1‑130 (MQ=255)
                                                                                                                      tGCTGGCGCGGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGa                           <  1:99615/130‑1 (MQ=255)
                                                                                                                            cgcgGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg              <  1:442544/137‑1 (MQ=255)
                                                                                                                            cgcgGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACcgcg              >  2:442544/1‑137 (MQ=255)
                                                                                                                              cgGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt         >  1:807183/1‑140 (MQ=255)
                                                                                                                              cgGGAAGACTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGAtt         >  1:820635/1‑140 (MQ=255)
                                                                                                                                     aCTACCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCttt  >  2:727534/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GCGGATATTCGTGAGGTCGCCTTTGCGGAATATGGCCGACTGAAACAAGTGGTACGCGAAGAGGATATCTGGCGCGGCGGTTTTTCCAGTTCGTCGGGTTATGAACTGGCAAAACAAATGCTGGCGCGGGAAGACTATCCGAAGGCACTGTTTGTTGCTTCCGATTCCATTGCTATCGGCGTACTGCGGGCAATTCATGAACGAGGCCTGAACATCCCACAGGATATTTCGCTTATCAGCGTTAACGATATCCCCACCGCGCGATTTACCTTT  >  NC_000913/3222030‑3222302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A