Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F28 I1 R1 172 67.8 2301510 98.9% 2276193 138.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,106 (C)5→6 noncoding (60/87 nt) leuV ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,1011.C100.0% 183.0 / NA 53noncoding (65/87 nt)leuVtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (28/25);  total (28/25)

TTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAA‑CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTC  >  NC_000913/4605970‑4606232
                                                                                                                                    |                                                                                                                                   
ttttGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAA‑ccccccacg                                                                                                                            <  1:39042/140‑5 (MQ=255)
     cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGt                                                                                                                        >  2:192699/1‑140 (MQ=255)
     cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGt                                                                                                                        <  2:126604/140‑1 (MQ=255)
     cTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGt                                                                                                                        >  2:1039184/1‑140 (MQ=255)
             gTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGacac                                                                                                                >  2:668802/1‑140 (MQ=255)
                ccTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATCGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTaa                                                                                                             <  1:1083473/140‑1 (MQ=39)
                     cACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCt                                                                                                        >  2:22133/1‑140 (MQ=255)
                            tGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                                                                                 <  1:511316/140‑1 (MQ=255)
                                ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                                             >  2:923458/1‑140 (MQ=255)
                                ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                                                                             >  1:92935/1‑140 (MQ=255)
                                 gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                                            <  2:92935/140‑1 (MQ=255)
                                 gCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                                            >  2:236974/1‑140 (MQ=255)
                                  catcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGG‑AGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                                          >  2:837294/1‑140 (MQ=37)
                                              gatAATGAATTTGAATCAGGGATGAAAAATCAAGG‑AGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                              <  1:837294/140‑1 (MQ=25)
                                                  aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                                                                           >  1:149237/1‑140 (MQ=255)
                                                   tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                          >  2:902553/1‑140 (MQ=255)
                                                   tGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                          >  2:864669/1‑140 (MQ=255)
                                                     aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                                        <  1:902553/140‑1 (MQ=255)
                                                      aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                                       >  1:1142165/1‑140 (MQ=255)
                                                      aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                                       >  2:835905/1‑140 (MQ=255)
                                                          gAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcac                                                                   <  2:1142165/140‑1 (MQ=255)
                                                               aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCa                                                              >  1:589715/1‑140 (MQ=255)
                                                               aGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCa                                                              >  2:1139931/1‑140 (MQ=255)
                                                                 ggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATc                                                            >  2:133232/1‑140 (MQ=255)
                                                                    tGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTa                                                         <  1:133232/140‑1 (MQ=255)
                                                                     gAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAc                                                        >  2:403317/1‑140 (MQ=255)
                                                                               ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGat                                              <  1:1139931/140‑1 (MQ=255)
                                                                                gaagaaACAAGAAATGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGata                                             <  2:802980/140‑1 (MQ=21)
                                                                                      aCAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCt                                       <  1:923458/140‑1 (MQ=255)
                                                                                        aaGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCg                                     <  2:823824/140‑1 (MQ=255)
                                                                                          gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                                   <  1:835905/140‑1 (MQ=255)
                                                                                          gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                                   <  2:127533/140‑1 (MQ=255)
                                                                                          gAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGtt                                   <  2:149237/140‑1 (MQ=255)
                                                                                            aaGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                                           <  1:214728/130‑1 (MQ=255)
                                                                                            aaGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATc                                           >  2:214728/1‑130 (MQ=255)
                                                                                              ggAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGt                               >  1:1042163/1‑140 (MQ=255)
                                                                                               gAAGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTg                              >  1:314576/1‑140 (MQ=255)
                                                                                                 aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg                            <  1:864669/140‑1 (MQ=255)
                                                                                                 aGTAAAGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCg                            <  2:314576/140‑1 (MQ=255)
                                                                                                      aGATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggg                       >  1:450575/1‑140 (MQ=255)
                                                                                                       gATAATTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAggggg                      <  1:403317/140‑1 (MQ=255)
                                                                                                          aaTTGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAgggggggg                   >  1:612897/1‑140 (MQ=255)
                                                                                                            ttGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGAc                 >  1:907271/1‑140 (MQ=255)
                                                                                                             tGGTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACt                >  1:21497/1‑140 (MQ=255)
                                                                                                              ggTGCGA‑GGGGGGGACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACtt               <  2:21497/140‑1 (MQ=255)
                                                                                                                     agggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGCTTTGAccc         >  1:911034/2‑140 (MQ=21)
                                                                                                                      gggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAAcccc        >  2:581940/1‑140 (MQ=255)
                                                                                                                      gggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAAcccc        <  2:1130684/140‑1 (MQ=255)
                                                                                                                       ggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAAccccc       <  2:907271/140‑1 (MQ=255)
                                                                                                                       ggggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGGTTGGTGCGAGGGGGGGGACTTGAAccccc       <  1:22133/140‑1 (MQ=255)
                                                                                                                        gggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCa      <  2:450575/140‑1 (MQ=255)
                                                                                                                        gggggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCa      <  1:851049/140‑1 (MQ=255)
                                                                                                                          gggACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg    >  1:1092807/1‑140 (MQ=255)
                                                                                                                            gACTTGAACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTc  >  1:1006604/1‑140 (MQ=255)
                                                                                                                                    |                                                                                                                                   
TTTTGCTACCATCGTGCCTGGCACTTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGCGAGGGGGGGGACTTGAA‑CCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTC  >  NC_000913/4605970‑4606232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A