Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,970,592	T→C	V58A (GTT→GCT)	ygdQ →	UPF0053 inner membrane protein YgdQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,970,592	0	T	C	100.0%	190.5 / NA	53	V58A (GTT→GCT)	ygdQ	UPF0053 inner membrane protein YgdQ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (27/26); total (27/26)

TTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGTTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTCCTTATCTGGAAA  >  NC_000913/2970490‑2970725

ttCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGtt                                                                                                  <  2:507695/140‑1 (MQ=255)

ttCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGtt                                                                                                  >  2:179378/1‑140 (MQ=255)

ttCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGtt                                                                                                  >  2:149900/1‑140 (MQ=255)

           gACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGAc                                                                                       >  2:885534/1‑140 (MQ=255)

                 attattTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATcc                                                                                 <  1:885534/140‑1 (MQ=255)

                  ttattTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCg                                                                                <  1:526381/140‑1 (MQ=255)

                  ttattTTCCTTTCTCCGGTGGTGGAATAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGTGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCGTGACAAATCCg                                                                                <  2:259122/140‑1 (MQ=255)

                    attTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCt                                                                              <  1:899847/140‑1 (MQ=255)

                       ttCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCtttt                                                                           >  2:927529/1‑140 (MQ=255)

                          cTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTAc                                                                        <  1:149900/140‑1 (MQ=255)

                            ttCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACaa                                                                      >  2:597851/1‑140 (MQ=255)

                             tctcTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAat                                                                     >  2:824600/1‑140 (MQ=255)

                             tctcTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAat                                                                     >  2:842405/1‑140 (MQ=255)

                                ctGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATAtt                                                                  <  1:235769/140‑1 (MQ=255)

                                ctGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATAtt                                                                  <  1:597851/140‑1 (MQ=255)

                                   gtggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcag                                                               >  2:847540/1‑140 (MQ=255)

                                    tggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATAtt                                                                  <  1:54824/136‑1 (MQ=255)

                                    tggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATAtt                                                                  >  2:54824/1‑136 (MQ=255)

                                    tggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              <  1:377879/140‑1 (MQ=255)

                                    tggtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              <  1:826765/140‑1 (MQ=255)

                                      gtggCAAAGCTTCCCACAGCACTACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagtca                                                            >  1:480752/1‑140 (MQ=255)

                                      gtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagtca                                                            <  2:8672/140‑1 (MQ=255)

                                      gtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagtca                                                            <  1:847540/140‑1 (MQ=255)

                                      gtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              <  1:439617/138‑1 (MQ=255)

                                      gtggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              >  2:439617/1‑138 (MQ=255)

                                        ggCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAgg                                                          >  1:170025/1‑140 (MQ=255)

                                         gCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGa                                                         >  1:137708/1‑140 (MQ=255)

                                              gCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              <  2:765548/130‑1 (MQ=255)

                                              gCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATACGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATtcagt                                                              >  1:765548/1‑130 (MQ=255)

                                               cTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTc                                                   <  1:927529/140‑1 (MQ=255)

                                                   ccACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGcc                                               >  1:490381/1‑140 (MQ=255)

                                                   ccACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGcc                                               <  1:842405/140‑1 (MQ=255)

                                                        gCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGTCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtga                                          >  2:638642/1‑140 (MQ=255)

                                                         cacaACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTCTTACAATATTCAGTCAGGAAATTTCTGCCCGtgat                                         >  1:662913/1‑140 (MQ=255)

                                                           caACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgattt                                       <  1:824600/140‑1 (MQ=255)

                                                            aaCGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCAT‑AATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgattt                                       >  1:545951/1‑138 (MQ=255)

                                                            aaCGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCAT‑AATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgattt                                       <  2:545951/138‑1 (MQ=255)

                                                              cGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgatttgat                                    >  2:69430/1‑140 (MQ=255)

                                                               gTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGTCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGtgatttgatt                                   <  1:638642/140‑1 (MQ=255)

                                                                 gCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCt                                 >  1:239663/1‑140 (MQ=255)

                                                                 gCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCt                                 >  2:121688/1‑140 (MQ=255)

                                                                   tCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGc                               >  2:492959/1‑140 (MQ=255)

                                                                         gcgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTgg                         <  2:239663/140‑1 (MQ=255)

                                                                          cgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTggg                        >  2:62858/1‑140 (MQ=255)

                                                                          cgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTggg                        >  1:142402/1‑140 (MQ=255)

                                                                          cgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTggg                        >  2:11459/1‑140 (MQ=255)

                                                                          cgcCGTCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTggg                        >  2:191717/1‑140 (MQ=255)

                                                                               tCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCt                   <  1:756372/140‑1 (MQ=255)

                                                                               tCTGGGGTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCt                   <  1:492959/140‑1 (MQ=255)

                                                                                    ggTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTc              <  1:368349/140‑1 (MQ=255)

                                                                                     gTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTcc             <  2:142402/140‑1 (MQ=255)

                                                                                     gTTGGCGGGAGCCATGGCTATGCGTCTGGCGCTGCAGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTCTTACAATATTCAGTCAGCAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTcc             <  2:662913/140‑1 (MQ=255)

                                                                                         gCGGGAGCCATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTCCTTAt         >  1:629133/1‑140 (MQ=255)

                                                                                                ccATGGCTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTCCTTATCTGGaaa  <  1:179378/140‑1 (MQ=255)

TTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTCTGGGGTTGGCGGGAGCCATGGTTATGCGTCTGGCGCTGCTGGCATCAATCGCCTGGGTTACGCGCCTGACAAATCCGCTTTTTACAATATTCAGTCAGGAAATTTCTGCCCGTGATTTGATTCTGCTTCTGGGTGGCTTGTTCCTTATCTGGAAA  >  NC_000913/2970490‑2970725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A