Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,896	G→A	P233S (CCT→TCT)	yqeF ←	putative acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,896	0	G	A	100.0%	150.5 / NA	43	P233S (CCT→TCT)	yqeF	putative acyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/18); total (25/18)

GCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGC  >  NC_000913/2984765‑2985028

gcgAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAAc                                                                                                                              >  2:243661/1‑140 (MQ=255)

              gCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCt                                                                                                                <  2:898705/140‑1 (MQ=255)

              gCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCt                                                                                                                >  1:792756/1‑140 (MQ=255)

                aCGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTgc                                                                                                              <  1:243661/140‑1 (MQ=255)

                    gTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTg                                                                                                          >  1:161500/1‑140 (MQ=255)

                         ttCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTc                                                                                                     >  1:702218/1‑140 (MQ=255)

                                          ggCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCAt                                                                                    <  2:161500/140‑1 (MQ=255)

                                             ttCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCgg                                                                                 <  1:863696/140‑1 (MQ=255)

                                               cGCTCATCATCATTACCGCAGCTGCGCGATCGTTTATGGATGATGCATTACCCGCTGTCTCAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTa                                                                               <  2:903912/140‑1 (MQ=255)

                                                       atcatTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCaacaac                                                                       <  2:702218/140‑1 (MQ=255)

                                                        tcatTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACTGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAAcaacc                                                                      <  1:816932/140‑1 (MQ=255)

                                                            tACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACg                                                                  >  2:806529/1‑140 (MQ=255)

                                                               cGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCt                                                               <  1:97088/140‑1 (MQ=255)

                                                                gCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTg                                                              >  1:766047/1‑140 (MQ=255)

                                                                 cAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTg                                                              <  2:166878/139‑1 (MQ=255)

                                                                 cAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTg                                                              >  1:166878/1‑139 (MQ=255)

                                                                   gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAAcaacca                                                                     <  1:133517/130‑1 (MQ=255)

                                                                   gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAAcaacca                                                                     >  2:133517/1‑130 (MQ=255)

                                                                   gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCt                                                               >  1:72862/1‑136 (MQ=255)

                                                                   gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCt                                                               <  2:72862/136‑1 (MQ=255)

                                                                   gCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGccc                                                           >  2:625882/1‑140 (MQ=255)

                                                                    cTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCg                                                          >  2:825576/1‑140 (MQ=255)

                                                                      gcgcCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGtt                                                        >  1:170186/1‑140 (MQ=255)

                                                                       cgcCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTa                                                       >  2:227518/1‑140 (MQ=255)

                                                                        gcCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTAc                                                      >  1:763130/1‑140 (MQ=255)

                                                                               tttATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGt                                               <  1:654300/140‑1 (MQ=255)

                                                                                    ggATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTa                                          <  2:766047/140‑1 (MQ=255)

                                                                                      atgatgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTGCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTAcc                                        >  1:840424/1‑140 (MQ=255)

                                                                                         atgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCggg                                     >  1:376985/1‑140 (MQ=255)

                                                                                          tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTc                                              >  1:522860/1‑130 (MQ=255)

                                                                                          tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTc                                              <  2:522860/130‑1 (MQ=255)

                                                                                               tACCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtc                               >  2:897299/1‑140 (MQ=255)

                                                                                                aCCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct                              >  1:234072/1‑140 (MQ=255)

                                                                                                aCCCGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct                              <  2:840424/140‑1 (MQ=255)

                                                                                                 cccGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtctc                             >  1:778564/1‑140 (MQ=255)

                                                                                                 cccGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct                              >  1:835351/1‑139 (MQ=255)

                                                                                                 cccGCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGAtct                              <  2:835351/139‑1 (MQ=255)

                                                                                                    gCTGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATc                          >  1:947226/1‑140 (MQ=255)

                                                                                                      tGTCACAGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCtt                        >  2:318219/1‑140 (MQ=255)

                                                                                                         cacaGAACCGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTaa                     <  1:318219/140‑1 (MQ=255)

                                                                                                                 cGAGACTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCg             <  1:625882/140‑1 (MQ=255)

                                                                                                                   agaCTATCAAATGAAGAATTTAAACGGGCTAAGCCTTCTGCACTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGc           >  1:228514/1‑140 (MQ=255)

                                                                                                                            aaTGAAGAATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgc  >  2:858001/1‑140 (MQ=255)

GCGAATGCGGGCCAGCACGGGTAAATTCAACGCTCGTGCTTTGGCTTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGC  >  NC_000913/2984765‑2985028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A