Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,986,581	A→G	V245V (GTA→GTG)	yqeG →	putative transporter YqeG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,986,581	0	A	G	100.0%	148.4 / NA	42	V245V (GTA→GTG)	yqeG	putative transporter YqeG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/24); total (18/24)

GGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCG  >  NC_000913/2986449‑2986711

ggTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCt                                                                                                                             >  2:424986/1‑140 (MQ=255)

ggTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCt                                                                                                                             <  1:424986/140‑1 (MQ=255)

            tAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCt                                                                                                                       >  2:876317/1‑134 (MQ=255)

            tAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCt                                                                                                                       <  1:876317/134‑1 (MQ=255)

                   aCGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCtt                                                                                                                      <  2:59011/128‑1 (MQ=255)

                   aCGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCtt                                                                                                                      >  1:59011/1‑128 (MQ=255)

                   aCGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCt                                                                                                          >  1:98519/1‑140 (MQ=255)

                    cGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCtt                                                                                                         <  2:405308/140‑1 (MQ=255)

                          ttattTCTTCGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTAtt                                                                                                   <  2:682232/140‑1 (MQ=255)

                          ttattTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTAtt                                                                                                   <  1:613398/140‑1 (MQ=255)

                            attTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTcc                                                                                                 <  2:98519/140‑1 (MQ=255)

                             tttCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCa                                                                                                <  2:903689/140‑1 (MQ=255)

                                 tACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCtt                                                                                            <  1:46083/140‑1 (MQ=255)

                                 tACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCtt                                                                                            >  1:74394/1‑140 (MQ=255)

                                   cGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCg                                                                                          >  1:329646/1‑140 (MQ=255)

                                     tttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCa                                                                                                <  2:513241/132‑1 (MQ=255)

                                     tttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCa                                                                                                >  1:513241/1‑132 (MQ=255)

                                     tttGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCA‑CTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGtat                                                                                       >  1:609284/1‑140 (MQ=255)

                                       tGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTc                                                                                                  <  1:873775/128‑1 (MQ=255)

                                       tGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTc                                                                                                  >  2:873775/1‑128 (MQ=255)

                                       tGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGtata                                                                                      >  1:354168/1‑140 (MQ=255)

                                            tAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGaa                                                                                 <  2:209069/140‑1 (MQ=255)

                                            tAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGaa                                                                                 <  2:354168/140‑1 (MQ=255)

                                             agacagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCA‑CTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgc                                                                               <  1:660228/140‑1 (MQ=255)

                                              gacagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgc                                                                               <  2:322469/140‑1 (MQ=255)

                                              gacagacGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAgc                                                                               <  2:329646/140‑1 (MQ=255)

                                                   acGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTa                                                                          >  1:563752/1‑140 (MQ=255)

                                                                ggCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGt                                                             <  2:265101/140‑1 (MQ=255)

                                                                               gATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGgc                                              >  1:228577/1‑140 (MQ=255)

                                                                               gATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGgc                                              <  2:641998/140‑1 (MQ=255)

                                                                                     tGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTc                                        >  1:904727/1‑140 (MQ=255)

                                                                                      gCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCt                                       <  2:228577/140‑1 (MQ=255)

                                                                                       cAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTa                                      >  2:331954/1‑140 (MQ=255)

                                                                                       cAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTa                                      >  2:940700/1‑140 (MQ=255)

                                                                                         aaaaaaTTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTAtg                                    >  1:314904/1‑140 (MQ=255)

                                                                                         aaaaaaTTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTAtg                                    >  1:277732/1‑140 (MQ=255)

                                                                                                tATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGa                             <  1:940700/140‑1 (MQ=255)

                                                                                                      aGTCGCTTATCTCATCATCTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGccc                       <  2:74394/140‑1 (MQ=255)

                                                                                                          gCTTATCTCATCA‑CTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCa                  <  2:609284/140‑1 (MQ=255)

                                                                                                                   atcatcTGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTc          >  2:381292/1‑140 (MQ=255)

                                                                                                                         tGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTc    <  2:337878/140‑1 (MQ=255)

                                                                                                                         tGCATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTc    <  2:904727/140‑1 (MQ=255)

                                                                                                                           cATAAGTGTGCTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCg  >  1:909070/1‑140 (MQ=255)

GGTTTTCGCCTTTAGCCATACGCCCATTATTTCTACGTTTGCCATAGACAGACGTGAAAAATATGGCGAACACGCTATGGATAAATGCAAAAAAATTATGAAAGTCGCTTATCTCATCATCTGCATAAGTGTACTGTTCTTTGTCTTTAGCTGCCTGCTTTCTATTCCACCTTCGTATATTGAAGCTGCTAAAGAAGAAGGGGTCACCATTTTATCGGCGCTTTCTATGCTGCCGAACGCCCCAGCATGGTTGTCAATTTCCG  >  NC_000913/2986449‑2986711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A