Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	182	57.1	1926220	99.1%	1908884	138.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,316,944	A→G	V92A (GTA→GCA)	phnM ←	RPnTP hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,316,944	0	A	G	100.0%	196.1 / NA	55	V92A (GTA→GCA)	phnM	RPnTP hydrolase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/33); total (22/33)

CGCAGCGCAGATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGC  >  NC_000913/4316807‑4317078

cgcagcgcagATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCg                                                                                                                                      >  2:722610/1‑140 (MQ=255)

cgcagcgcagATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCg                                                                                                                                      <  2:697311/140‑1 (MQ=255)

       cagATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGa                                                                                                                               <  2:817931/140‑1 (MQ=255)

       cagATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGa                                                                                                                               <  2:308326/140‑1 (MQ=255)

             cAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGc                                                                                                                         <  2:822538/140‑1 (MQ=255)

               gACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTc                                                                                                                       <  2:279173/140‑1 (MQ=255)

                 cGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGc                                                                                                                     <  2:173213/140‑1 (MQ=255)

                 cGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGc                                                                                                                     <  1:319881/140‑1 (MQ=255)

                    tGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGccac                                                                                                                  >  1:921779/1‑140 (MQ=255)

                    tGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGccac                                                                                                                  >  1:78498/1‑140 (MQ=255)

                    tGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGccac                                                                                                                  >  1:181944/1‑140 (MQ=255)

                      cTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCTGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGccacca                                                                                                                >  2:69585/1‑140 (MQ=255)

                      cTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGc                                                                                                                         <  1:41676/131‑1 (MQ=255)

                      cTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGc                                                                                                                         >  2:41676/1‑131 (MQ=255)

                          gcgcGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAg                                                                                                            <  2:402416/140‑1 (MQ=255)

                               gTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAACCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGcgt                                                                                                       <  2:864841/140‑1 (MQ=255)

                                 tGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGcgtcg                                                                                                     >  2:555723/1‑140 (MQ=255)

                                    cgccgcGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTgg                                                                                                  <  2:285017/140‑1 (MQ=255)

                                     gccgcGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCTGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGgc                                                                                                 <  1:69585/140‑1 (MQ=255)

                                        gcgTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGgctgc                                                                                              <  1:863211/140‑1 (MQ=255)

                                          gTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTc                                                                                            >  2:811848/1‑140 (MQ=255)

                                                  gTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGa                                                                                    <  1:67327/140‑1 (MQ=255)

                                                  gTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGa                                                                                    <  1:811848/140‑1 (MQ=255)

                                                     tctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGgctgc                                                                                              >  2:483150/1‑127 (MQ=255)

                                                     tctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGgctgc                                                                                              <  1:483150/127‑1 (MQ=255)

                                                      ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATc                                                                                         <  1:609350/131‑1 (MQ=255)

                                                      ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATc                                                                                         >  2:609350/1‑131 (MQ=255)

                                                      ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTgg                                                                                >  2:161060/1‑140 (MQ=255)

                                                      ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTgg                                                                                >  1:86097/1‑140 (MQ=255)

                                                      ctTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTgg                                                                                >  1:298315/1‑140 (MQ=255)

                                                         cGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCa                                                                             <  2:921779/140‑1 (MQ=255)

                                                             ggCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGcc                                                                         >  1:628769/1‑140 (MQ=255)

                                                                gTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGt                                                                      <  2:78498/140‑1 (MQ=255)

                                                                gTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGt                                                                      <  2:86097/140‑1 (MQ=255)

                                                                gTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGt                                                                      <  2:590923/140‑1 (MQ=255)

                                                                        tcttctCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTc                                                              >  1:234579/1‑140 (MQ=255)

                                                                        tcttctCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTc                                                              >  1:208713/1‑140 (MQ=255)

                                                                            ctcCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGgc                                                          >  1:151202/1‑140 (MQ=255)

                                                                             tcCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGgcg                                                         <  2:181944/140‑1 (MQ=255)

                                                                              ccAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGgcgc                                                        >  2:307216/1‑140 (MQ=255)

                                                                                  aTTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCgggg                                                    >  2:589042/1‑140 (MQ=255)

                                                                                    tctcCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTg                                                  <  2:571722/140‑1 (MQ=255)

                                                                                     ctcCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTga                                                 <  2:234579/140‑1 (MQ=255)

                                                                                      tcCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTgaa                                                >  2:481550/1‑140 (MQ=255)

                                                                                           ccGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAAtt                                           >  1:174685/1‑140 (MQ=255)

                                                                                                    gccgTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGAt                                  <  1:845831/140‑1 (MQ=255)

                                                                                                    gccgTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGAt                                  <  1:722610/140‑1 (MQ=255)

                                                                                                       gTCGCGCACGTCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTAt                               <  2:208713/140‑1 (MQ=255)

                                                                                                                 tCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAg                     <  2:174685/140‑1 (MQ=255)

                                                                                                                 tCGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAg                     <  1:307216/140‑1 (MQ=255)

                                                                                                                  cGCCAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGc                    >  1:204424/1‑140 (MQ=255)

                                                                                                                     cAATTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCa                 <  1:555723/140‑1 (MQ=255)

                                                                                                                      aaTTGCCACGGCATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCaa                <  2:38099/140‑1 (MQ=255)

                                                                                                                                 cATCCAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGgc     <  2:204424/140‑1 (MQ=255)

                                                                                                                                    ccAGTGCGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGgcagc  <  1:589042/140‑1 (MQ=255)

CGCAGCGCAGATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTACGGTGGTGATGCCGCTCGCCACCATCAGCGCGTCGTGGCTGCTCATCGCCGAGTGGGCAGGCCAGTCAACTTTCGGGCGCGGGGTGAAGAATTTATCCAGATTATCGGTATGCAGCTCAATCAGCCCCGGCAGC  >  NC_000913/4316807‑4317078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A